Me pēhea te wehe i te toto mai i te toto mā te whakamahi i te centrifuge
Kupu Whakataki:
He tikanga taiwhanga whānui te whakamahi i te centrifugation e wehewehe ana i ngā wāhanga rerekē o te tauira i runga i tō rātou mātotoru. I roto i te ao rongoā, he mahi nui tāna ki te wehe i te toto mai i te toto mō ngā take tātaritanga me ngā rangahau. Ka whakaratohia e tēnei tuhinga he aratohu taahiraa-i-te-taahiraa mō te wehewehe i te toto mai i te toto mā te whakamahi i te centrifuge, me ngā mahi whakatūpato e tika ana hei whakarite i ngā hua tika me te pono.
1. Te Mārama ki te Whakapūtanga me tōna Hiranga i roto i te Wehenga Huka
- Te whakamāramatanga me te mātāpono mahi o te centrifuge
- Te hiranga o te wehenga toto i roto i ngā tono hauora
I mua i te ruku hohonu ki te tukanga wehewehe i te toto mai i te toto, he mea nui kia mārama mārama ki te centrifugation me tōna hiranga i roto i tēnei horopaki. Ko te centrifuge he taputapu taiwhanga e whakamahi ana i te kaha centrifuge hei wehewehe i ngā matūriki i roto i te tauira wai i runga i tōna kiato, i tōna rahi rānei.
He mea nui te wehenga o te toto mai i te toto mō ngā momo rangahau hauora, pērā i ngā whakamātautau tātaritanga, ngā whakamātautau matū koiora, me ngā rangahau hauora. Ko te toto te wāhanga wai o te toto e toe ana i muri i te toto totoka, kāore he pūtau me ngā mea totoka. Kei roto he whānuitanga o ngā pūmua, ngā whākōkī, ngā homoni, me ngā katote ka taea te whakarato i ngā māramatanga whai hua mō te āhua hauora o te tangata.
2. Te Whakarite i ngā Tauira me ngā Taputapu
- Ngā rauemi e hiahiatia ana mō te wehenga toto
- Te whakahaere tika me te tapanga i ngā tauira toto
I mua i te tīmatanga o te tukanga centrifugation, kia mōhio kei a koe ngā rauemi katoa e tika ana. Kei roto i ēnei ko ngā ngongo kohikohi toto, he mīhini centrifugation, he taputapu haumaru tika (pēnei i ngā karapu me ngā koti taiwhanga), me ngā ngongo hei pupuri i te serum kua wehea.
He mea nui te whakahaere tika me te tapanga i ngā tauira toto kia kore ai e hē te tautuhi, e hapa rānei. Me tohu mārama ia tauira ki tētahi tohu motuhake, pērā i te ingoa o te tūroro, te tau tohu rānei, kia tika ai ngā hua, kia kore ai hoki e pōhēhē i te wā e tātarihia ana.
3. Te Whakatū i ngā Tawhā Pūwero
- Te whiriwhiri i ngā tautuhinga pūwero tika
- Te whakatau i te kaha me te wā o te pūwero e hiahiatia ana
Ko te mahi e whai ake nei ko te whakarite i ngā tawhā o te centrifuge kia tutuki ai te wehenga tino pai. Ko ngā tautuhinga centrifuge tika e whakawhirinaki ana ki ngā whakaritenga motuhake o te whakamātautau, o te tātari rānei e mahia ana.
Hei wehe i te toto mai i te toto, he nui te kaha whakatere i waenga i te 1,500 ki te 2,000 ngā wā o te kaha o te taumaha (e whakaatuhia ana ko te kaha whakatere whanaunga, RCF rānei). Ka rerekē pea te roa o te whakatere i runga i te rahinga me te momo tauira toto.
4. Te uta i te Centrifuge me te tīmata i te tukanga wehewehe
- Te whakahaere tika me te utaina o ngā tauira toto ki roto i te centrifuge
- Ngā tohutohu mō te pupuri i te taurite me te pumau i te wā e whakamahia ana te centrifugation
Āta utaina ngā tauira toto ki roto i te centrifuge, kia ōrite te tohatoha puta noa i te rotor kia mau tonu ai te taurite i te wā e whakamahia ana. He mea nui kia utaina he maha ōrite o ngā ngongo, kia whakamahia rānei he taumaha taurite hei pupuri i te pumau me te ārai i te kino o te centrifuge.
Kia tika te utaina o ngā tauira katoa, katia te taupoki o te centrifuge kia mau, ka tīmata te tukanga wehewehe. Ka huri tere te centrifuge i ngā tauira, ka whakamahia he kaha centrifuge kaha e meinga ai ngā toto toto whero kia tau ki raro o te ngongo, ko te toto toto māmā ake ka kohia ki runga.
5. Te Kohikohi me te Penapena i te Serum Wehewehe
- Ngā tikanga mō te kohikohinga tika o te serum
- Ngā tikanga pai mō te rokiroki i te toto hei pupuri i te kounga o te tauira
I muri i te whakapūtanga, tangohia marietia ngā ngongo mai i te pūpūtanga, kaua e whakararuraru i ngā paparanga kua wehea. Mā te whakamahi i tētahi pipette, i tētahi pūnaha kohikohinga toto motuhake rānei, tangohia te paparanga toto mai i ia ngongo, me te tūpato kia kore ai e poke.
Hei pupuri i te kounga o te tauira toto, he mea nui kia rongoatia tika. Me whakawhiti te toto ki ngā ngongo rokiroki ma, parakore hoki, he pai ake te hanga mai i ngā mea kore e pakaru pērā i te karāhe, kia kore ai e pokanoa ki ngā tātaritanga taiwhanga o muri mai. Me rongoa ngā ngongo kua tohua tika ki te pāmahana tika, ā, me tiaki hoki i te māramatanga kia iti ai te pirau.
Whakatau:
He tikanga tino nui te whakamahi i te centrifugation hei wehewehe i te toto mai i te toto i roto i ngā mahi hauora me ngā mahi rangahau. Mā te whai i te aratohu taahiraa-i-te-taahiraa kua whakaratohia i roto i tēnei tuhinga, ka taea e ngā kairangahau me ngā tohunga hauora te tiki pono i ngā tauira toto kounga teitei mō ngā whakamātautau tātaritanga tika me ngā rangahau pūtaiao. I te mea kei roto i te toto ngā mōhiohio whai hua e pā ana ki te hauora o te tangata, he mea nui te mōhio ki te toi o te wehenga toto mā te whakamahi i te centrifuge mō ngā taiwhanga katoa e mahi ana i roto i te mara o te rongoā.
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