yintoni umatshini we-centrifuge
Yintoni iCentrifuge Machine?
Intshayelelo
Umatshini we-centrifuge sisixhobo esibalulekileyo esisetyenziswa kumashishini ahlukeneyo nakwiilabhoratri. Usebenzisa umgaqo we-centrifugation ukwahlula izinto ezinobunzima obahlukeneyo ngaphakathi komxube wolwelo okanye wegesi. Le nkqubo ixhomekeke ekudalweni kwamandla e-centrifugal, okubangela ukuba amasuntswana axineneyo aphume, ngelixa lawo alula ehlala esondele embindini. Kweli nqaku, siza kujonga nzulu kumatshini we-centrifuge, sihlola umgaqo wawo wokusebenza, usetyenziso, iintlobo, kunye nezinto eziphambili.
Ukuqonda uMgaqo wokuSebenza
Umatshini we-centrifuge luyimveliso emangalisayo esebenzisa amandla asebenza kwinto ejikelezayo. Usebenzisa amandla e-centrifugal, alwa namandla adonsela phantsi, nto leyo ebangela ukwahlulwa kwezinto ezahlukeneyo. Lo matshini unedrama ejikelezayo ngesantya esiphezulu, ebizwa ngokuba yi-rotor, ebamba isampuli ukuba ijikeleziswe. Xa i-rotor ijikeleza ngokukhawuleza, kuveliswa amandla e-centripetal, etyhala amasuntswana aye kumda wangaphandle we-rotor ngelixa idala i-gradient yoxinano lwamasuntswana.
Izicelo kwiBiotechnology nakwiSayensi yezonyango
1. Uhlalutyo lweSampuli yeGazi
Enye indlela ephambili yokusebenzisa i-centrifugation kuhlalutyo lwesampuli yegazi. Idlala indima ebalulekileyo ekwahlukaniseni izinto zegazi, ezifana neeseli ezibomvu zegazi, iiseli ezimhlophe zegazi, iiplatelets, kunye ne-plasma. Ngokujikelezisa isampuli yegazi kwi-centrifuge, iiseli zegazi ezixineneyo zahlulwa kwi-plasma, okwenza kube lula ukuxilongwa kwelebhu kwaye kuvumele uhlalutyo olupheleleyo lweeparameter ezahlukeneyo.
2. Ukucocwa kweeMolekyuli zeBiological
I-Centrifugation isetyenziswa kakhulu ekucoceni iimolekyuli zebhayoloji. Umzekelo, ekukhutshweni kwe-DNA, i-centrifugation yahlula ngempumelelo i-DNA kwezinye izinto zeseli, ezifana neeproteni kunye nee-nucleic acids. Ngaphezu koko, ii-centrifuges zisetyenziselwa ukucoca iiproteni kunye nokwahlula ii-organelles ezithile kwi-homogenates zeseli, zinceda kuphando olunxulumene nebhayoloji yeseli kunye nebhayoloji yemolekyuli.
Iintlobo zeeMishini zeCentrifuge
1. I-Fixed Angle Rotor Centrifuge
I-fixed-angle rotor centrifuge luhlobo oluqhelekileyo lomatshini we-centrifuge. Ine-rotor ehlala kwi-engile ezinzileyo, ngokuqhelekileyo phakathi kwama-20 ukuya kuma-40 degrees. Olu yilo lwe-rotor luvumela ukwahlukana ngokukhawuleza kwamasuntswana ngenxa yamandla e-centrifugal asebenza ngokuthe nkqo kumphezulu weetyhubhu zesampuli. I-fixed-angle rotor centrifuges idla ngokusetyenziswa xa kusetyenzwa iisampulu ezincinci.
2. I-Swing-bucket Rotor Centrifuge
I-swing-bucket rotor centrifuge lolunye uhlobo olusetyenziswa kakhulu kwiilabhoratri. Iqulathe i-rotor enezinto ezifana nebhakethi ezijikajikayo xa kukhawuleziswa, nto leyo evumela iityhubhu zesampulu ukuba zihlale kwi-engile. Olu yilo lwenza kube lula ukuphatha isampuli kwaye lunciphisa amathuba okungcoliswa okunqamlezileyo. Ii-swing-bucket rotor centrifuge zifanelekile kwiisampuli ezinkulu kwaye zifumaneka rhoqo kwiilabhoratri zokuxilonga zeklinikhi.
Izinto ezibalulekileyo zoMshini weCentrifuge
1. I-Rotor
I-rotor yinxalenye ebalulekileyo yalo naliphi na umatshini we-centrifuge. Sisakhiwo esisilinda esikwaziyo ukujikeleza ngesantya esiphezulu. Uhlobo lwe-rotor esetyenziswayo luxhomekeke kwisicelo kunye neemfuno zomthamo wesampuli. Ii-rotor zingaba yi-engile esisigxina, ibhakethi yokujika, ezithe nkqo, okanye ezithe tye, nganye yenzelwe ukwamkela iintlobo ezithile zeesampuli.
2. Inkqubo yokuqhuba
Inkqubo yokuqhuba yomatshini we-centrifuge iguqula intshukumo yokujikeleza kwemoto ibe kukujikeleza okukhawulezayo okufunekayo ukuze kujikeleziswe i-centrifuge. Ihlala inemoto, ibhanti yokuqhuba, kunye nee-pulleys. Imoto iqhuba i-rotor ngokusebenzisa unxibelelwano loomatshini, iqinisekisa isantya sokujikeleza esihambelanayo nesithembekileyo ngexesha lenkqubo yokwahlulahlula.
3. Iphaneli yoLawulo
Oomatshini be-Centrifuge baxhotyiswe ngeephaneli zokulawula ezivumela abasebenzisi ukuba basete kwaye balawule iiparameter ezahlukeneyo. Ezi paneli zihlala zinezicwangciso zesantya, ixesha, ubushushu, kunye namazinga okukhawulezisa kunye nokuhla kwesantya. Iimodeli eziphambili zinokubandakanya iinkqubo ezimiselwe kwangaphambili kunye neempawu zokhuseleko, ezifana nezivalo kunye neenkqubo zokufumanisa ukungalingani.
4. Iimpawu zoKhuseleko
Oomatshini be-Centrifuge baquka iimpawu ezahlukeneyo zokhuseleko ukukhusela abasebenzisi kunye neesampuli. Ezi ziquka izivalo ezithintela ukuvulwa ngengozi ngexesha lokusebenza, iinkqubo zokufumanisa ukungalingani ezimisa umatshini ngoko nangoko ukuba umthwalo awulingani, kunye namaqhosha okuma ngokukhawuleza ukuze ucime ngokukhawuleza.
Isiphelo
Ukuqukumbela, umatshini we-centrifuge sisixhobo esimangalisayo esisetyenziswa kakhulu kumashishini nakwiilabhoratri. Ngokusebenzisa amandla e-centrifugal, wahlula ngokufanelekileyo izinto ezinobunzima obahlukeneyo ngaphakathi komxube, nto leyo evumela iinkqubo ezahlukeneyo zesayensi kunye nohlalutyo. Ukusuka kuhlalutyo lwesampulu yegazi ukuya ekukhutshweni kwe-DNA kunye nokucocwa kweproteni, umatshini we-centrifuge uhlala usisixhobo esibalulekileyo kwicandelo le-biotechnology kunye nesayensi yezonyango. Ngokuqonda umgaqo wayo wokusebenza, ukusetyenziswa, iintlobo, kunye nezinto eziphambili, sinokuyiqonda indima ebalulekileyo edlalwa ngumatshini we-centrifuge kuphuhliso lwesayensi kunye nophando.
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