mochini oa centrifuge ke eng

Mochini oa Centrifuge ke eng?

Selelekela

Mochini oa centrifuge ke sesebelisoa sa bohlokoa se sebelisoang liindastering le lilaboratoring tse fapaneng. O sebelisa molao-motheo oa centrifuge ho arola lintho tse nang le bongata bo fapaneng ka har'a motsoako oa metsi kapa oa khase. Ts'ebetso ena e itšetlehile ka tlhahiso ea matla a centrifuge, a etsang hore likaroloana tse teteaneng li tsamaee ka ntle, ha tse bobebe li lula haufi le bohareng. Sehloohong sena, re tla teba haholoanyane mochining oa centrifuge, re hlahlobe molao-motheo oa ts'ebetso ea ona, lits'ebetso, mefuta le likarolo tsa bohlokoa.

Ho Utloisisa Molao-motheo oa Mosebetsi

Mochini oa centrifuge ke tlhahiso e ikhethang e sebelisang matla a sebetsang nthong e potolohang. O sebelisa matla a centrifugal, a loantšang matla a khoheli, a fellang ka karohano ea lintho tse fapaneng. Mochini o na le moropa o potolohang ka lebelo le phahameng, o bitsoang rotor, o tšoarang sampole hore e centrifuge. Ha rotor e potoloha ka potlako, matla a centripetal a hlahisoa, a sutumelletsa likaroloana ho ea moeling o ka ntle oa rotor ha a ntse a etsa gradient ea bongata ba likaroloana.

Likopo ho Biotechnology le Saense ea Bongaka

1. Tlhahlobo ea Sampole ea Mali

Tšebeliso e 'ngoe ea mantlha ea centrifugation ke tlhahlobong ea sampole ea mali. E bapala karolo ea bohlokoa ho aroleng likarolo tsa mali, joalo ka lisele tse khubelu tsa mali, lisele tse tšoeu tsa mali, li-platelet le plasma. Ka ho potoloha sampole ea mali ka centrifuge, lisele tsa mali tse teteaneng li aroloa ho plasma, e leng se nolofatsang tlhahlobo ea laboratori le ho lumella tlhahlobo e felletseng ea liparamente tse fapaneng.

2. Tlhoekiso ea Limolek'hule tsa Baeloji

Ho centrifugation ho sebediswa haholo ho hlwekiseng dimolek'hule tsa baeloji. Mohlala, ho ntsheng DNA, ho centrifugation ho arola DNA ho dikarolo tse ding tsa sele ka katleho, tse kang diprotheine le di-nucleic acid. Ho feta moo, di-centrifuge di sebediswa ho hlwekisa diprotheine le ho arola di-organelle tse itseng ho di-homogenate tsa sele, e leng se thusang dipatlisisong tse amanang le baeloji ya sele le baeloji ya dimolek'hule.

Mefuta ea Mechini ea Centrifuge

1. Sentrifuge ea Angle Rotor e Tsitsitseng

Sentifuge ea rotor ea angle e tsitsitseng ke mofuta o sebelisoang haholo oa mochini oa centrifuge. E na le rotor e lulang e le ka lehlakoreng le tsitsitseng, hangata e pakeng tsa likhato tse 20 ho isa ho tse 40. Moralo ona oa rotor o lumella karohano e potlakileng ea likaroloana ka lebaka la matla a centrifugal a sebetsang ka ho otloloha holim'a lipeipi tsa sampole. Sentifuge ea rotor ea angle e tsitsitseng hangata e sebelisoa ha ho sebetsoa li-volume tse nyane tsa sampole.

2. Sentrifuge ea Rotor ea bakete e Swing-bakete

Sentrifuge ea rotor ea swing-bucket ke mofuta o mong o sebelisoang haholo lilaboratoring. E entsoe ka rotor e nang le lihokelo tse kang tsa bakete tse sothehang nakong ea ho potlaka, e leng se lumellang lipeipi tsa sampole ho lula ka lehlakoreng. Moralo ona o nolofalletsa ho sebetsana le sampole habonolo mme o fokotsa menyetla ea tšilafalo e tšelang. Sentrifuge ea rotor ea swing-bucket e loketse boholo ba sampole 'me hangata e fumanoa lilaboratoring tsa tlhahlobo ea bongaka.

Likarolo tsa Bohlokoa tsa Mochini oa Centrifuge

1. Rotor

Rotor ke karolo ea bohlokoa ea mochini ofe kapa ofe oa centrifuge. Ke sebopeho sa cylindrical se khonang ho potoloha ka lebelo le phahameng. Mofuta oa rotor o sebelisoang o itšetlehile ka litlhoko tsa ts'ebeliso le bophahamo ba sampole. Lirotor e ka ba tsa angle e tsitsitseng, bakete ea swing, tse otlolohileng, kapa tse otlolohileng, 'me e 'ngoe le e 'ngoe e etselitsoe ho amohela mefuta e itseng ea disampole.

2. Sistimi ea ho khanna

Sistimi ea ho khanna ea mochini oa centrifuge e fetola motsamao oa ho potoloha ha enjene hore e be ho potoloha ka lebelo le phahameng ho hlokahalang bakeng sa ho potoloha ka centrifuge. Hangata e na le enjene, lebanta la ho khanna le li-pulley. Enjene e khanna rotor ka khokahano ea mechini, e netefatsang lebelo le tsitsitseng le le tšepahalang la ho potoloha nakong ea ts'ebetso ea karohano.

3. Phanele ea Taolo

Mechini ea centrifuge e na le liphanele tsa taolo tse lumellang basebelisi ho beha le ho laola liparamente tse fapaneng. Liphanele tsena hangata li na le litlhophiso tsa lebelo, nako, mocheso, le sekhahla sa ho potlaka le ho fokotseha ha lebelo. Mefuta e tsoetseng pele e ka boela ea kenyelletsa mananeo a seng a behiloe esale pele le likarolo tsa polokeho, joalo ka liloko tsa sekoahelo le litsamaiso tsa ho lemoha ho se leka-lekane.

4. Likarolo tsa Polokeho

Mechini ea centrifuge e kenyelletsa likarolo tse fapaneng tsa polokeho ho sireletsa basebelisi le lisampole. Tsena li kenyelletsa liloko tsa sekwahelo tse thibelang ho buleha ka phoso nakong ea ts'ebetso, litsamaiso tsa ho lemoha ho se leka-lekane tse emisang mochini hang-hang haeba mojaro o sa leka-lekane, le likonopo tsa ho emisa ka tšohanyetso bakeng sa ho tima hang-hang.

Qetello

Qetellong, mochini oa centrifuge ke sesebelisoa se ikhethang se fumanang lits'ebetso tse ngata liindastering le lilaboratoring. Ka ho sebelisa matla a centrifugal, o arola ka katleho lintho tse nang le bongata bo fapaneng ka har'a motsoako, o nolofalletsa lits'ebetso tse fapaneng tsa saense le tsa tlhahlobo. Ho tloha tlhahlobong ea sampole ea mali ho isa ho ntšoeng ha DNA le tlhoekisong ea protheine, mochini oa centrifuge o lula e le sesebelisoa sa bohlokoa masimong a theknoloji ea baeloji le mahlale a bongaka. Ka ho utloisisa molao-motheo oa eona oa ts'ebetso, lits'ebetso, mefuta le likarolo tsa bohlokoa, re ka ananela karolo ea bohlokoa eo mochini oa centrifuge o e bapalang tsoelo-peleng ea saense le lipatlisisong.