indlela yokwahlula nge-centrifugation
Intshayelelo kwiCentrifugation kunye nokubaluleka kwayo kwiinkqubo zokwahlukana
I-Centrifugation yindlela esetyenziswa kakhulu kumashishini ahlukeneyo nakwiindawo zophando ukwahlula imixube eyahlukeneyo ngokusekelwe kubukhulu beesuntswana kunye nobuninzi bazo. Nokuba kukwiilebhu zonyango, kumashishini amayeza, okanye kuphando lokusingqongileyo, ukubaluleka kwe-centrifugation ekufezekiseni ukwahlukana okusebenzayo akunakugxininiswa kakhulu.
Eli nqaku lijolise ekunikezeni isikhokelo esipheleleyo sendlela yokwahlula izinto kusetyenziswa i-centrifugation. Siza kuxoxa ngomgaqo we-centrifugation, sihlolisise iintlobo ezahlukeneyo ze-centrifuges, sihlolisise iindlela ezahlukeneyo zokwahlula, sigxininise izinto eziphambili ezichaphazela ukusebenza kakuhle kokwahlula, size sigqibezele ngezinye iindlela zokusetyenziswa kwale ndlela.
Ukuqonda uMgaqo weCentrifugation
I-Centrifugation isebenza ngokomgaqo we-sedimentation, apho ukusetyenziswa kwamandla e-centrifugal kubangela ukuba amasuntswana axineneyo ahambe aye ezantsi, enze i-pellet, ngelixa amasuntswana akhanyayo ehlala kwi-supernatant. Olu hluko lufezekiswa ngenxa yomahluko kubuninzi bamasuntswana, imo, ubungakanani, kunye nokusebenzisana kwawo nendawo ejikelezileyo.
Iintlobo zeeCentrifuges kunye nezicelo zazo
Kukho iintlobo ezahlukeneyo zee-centrifuge ezikhoyo, kuquka ii-ultracentrifuge, ii-microcentrifuge, kunye nee-high-speed centrifuge, nganye yenzelwe ukwanelisa iimfuno ezithile zokwahlulwahlulwa. Ii-Ultracentrifuge, umzekelo, ziyakwazi ukuvelisa amandla aphezulu kakhulu e-centrifugal, okuvumela ukwahlulwahlulwa kwamasuntswana kuluhlu lwe-nanometer. Ngokwahlukileyo koko, ii-microcentrifuge zincinci kwaye zisetyenziswa kakhulu kwiilabhoratri zeklinikhi zokwahlulwahlulwa kwesampuli rhoqo.
Iindlela zokuHlahlula ezisetyenziswa kwiCentrifugation
I-Centrifugation isebenzisa iindlela ezahlukeneyo zokwahlula, kuxhomekeke kuhlobo lwesampulu kunye nesiphumo esifunekayo. Ezi ndlela ziquka i-differential centrifugation, i-rate-zonal centrifugation, i-density gradient centrifugation, kunye ne-isopycnic centrifugation.
Ukwahlulahlula i-centrifugation kwenza ukuba amasuntswana ahlukane ngokusekelwe kubukhulu bawo kunye noxinano lwawo. Ngokubeka umxube kwimijikelo elandelelanayo ye-centrifugation ngesantya esonyukayo, amasuntswana amancinci aqala ngokulahla inkunkuma, kulandele amasuntswana amakhulu, nto leyo ekhokelela ekuhlukaneni.
Kwelinye icala, i-centrifugation yezinga-zonal ibandakanya ukulayisha isampuli kwi-gradient medium enoxinano oluhlukeneyo. I-centrifugation emva koko ibangela ukuba amasuntswana afuduke kwi-gradient ngokusekelwe kubukhulu kunye nokuma kwawo, ekugqibeleni enze amabhanti ahlukeneyo.
I-centrifugation ye-density gradient isebenzisa i-density gradients eyenziwe zizinto ezifana ne-sucrose okanye i-cesium chloride. Ii-particles ziphuma kwi-gradient zide zifikelele kwindawo enoxinano olulinganayo, nto leyo ekhokelela ekwahlukaneni. Le ndlela iluncedo ngakumbi xa kwahlula ii-organelles okanye iintlobo ezahlukeneyo zeeseli.
Kwi-isopycnic centrifugation, amasuntswana ahlulwa ngokusekelwe kuxinano lwawo oluthambileyo. Ngokufaka isampuli kwi-gradient apho amasuntswana anoxinano olufanayo nolwendawo ejikelezileyo, amasuntswana ayadada okanye atshone de kufikelelwe kukulingana. Le ndlela ifumana ukusetyenziswa kwi-DNA kunye ne-RNA yokwahlulwa.
Izinto Ezichaphazela Ukusebenza Kakuhle kweCentrifugation
Izinto ezininzi zichaphazela ukusebenza kakuhle nokusebenza kakuhle kwe-centrifuge. Ezi ziquka isantya kunye nexesha le-centrifuge, ubushushu, uhlobo lwe-rotor kunye nobukhulu, umthamo wesampuli, kunye nokukhethwa kwendlela yokwahlula. Ukuphucula ezi zinto ziguquguqukayo kubalulekile ekufezekiseni iziphumo zokwahlula ezichanekileyo nezihambelanayo.
Ukusetyenziswa kweCentrifugation kwiinkalo ezahlukeneyo
I-Centrifugation ineendlela ezahlukeneyo zokusetyenziswa kwiindawo ezahlukeneyo. Kwiilabhoratri zonyango, isetyenziselwa ukwahlulwa kwesampuli yegazi, okuvumela ukwahlulwa kwe-plasma okanye i-serum ngeenjongo zokuxilonga. Kwishishini lamayeza, i-centrifugation inceda kwiinkqubo zokucoca, yahlula izithako ezisebenzayo kokungcola. Uphando lokusingqongileyo lusebenzisa i-centrifugation ukuhlalutya iisampuli zedaka, okuvumela ukuchongwa kokungcola kunye neentsholongwane.
Ukuqukumbela, i-centrifugation idlala indima ebalulekileyo kwiinkqubo zokwahlulahlula, inika isixhobo esinamandla kubaphandi kunye namashishini kumacandelo ahlukeneyo. Ukuqonda imigaqo, iintlobo, kunye neendlela zokuhlukanisa, kunye nokwenza ngcono izinto eziphambili, kunokukhokelela ekuhlukaniseni okusebenzayo kunye nokuvelisa iziphumo ezichanekileyo. Ngokusebenzisa amandla okuhlukanisa, izazinzulu ziyaqhubeka nokuvelisa amathuba amatsha kwicandelo lesayensi yokwahlulahlula.
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