nzira yekuparadzanisa ne centrifugation

Nhanganyaya yeCentrifugation uye Kukosha Kwayo Mumaitiro Ekuparadzanisa

Kubatanidza zvinhu (centrifugation) inzira inoshandiswa zvakanyanya mumaindasitiri akasiyana-siyana uye munzvimbo dzekutsvagisa pakupatsanura misanganiswa yakasiyana-siyana zvichienderana nehukuru hwezvinhu zvacho uye kuwanda kwazvo. Ingave mumarabhoritari ekurapa, maindasitiri emishonga, kana ongororo yezvakatipoteredza, kukosha kwekubatanidza zvinhu (centrifugation) mukuwana kupatsanurana kwakanaka hakufanirwe kutaurwa zvakanyanya.

Chinyorwa chino chine chinangwa chekupa gwara rakazara rekuti tingaparadzanisa sei zvinhu zvinobudirira tichishandisa centrifugation. Tichakurukura musimboti we centrifugation, tichaongorora mhando dzakasiyana dze centrifuges, tichaongorora nzira dzakasiyana dzekuparadzanisa, tosimbisa zvinhu zvakakosha zvinokanganisa mashandiro ekuparadzanisa, uye topedzisa nekushandisa nzira iyi.

Kunzwisisa Nheyo yeCentrifugation

Kubatanidza zvinhu kunoshanda panheyo yekudonha kwevhu, uko kushandiswa kwesimba repakati kunoita kuti zvidimbu zvakakora zvifambe zvichienda pasi, zvichigadzira pellet, nepo zvidimbu zvakareruka zvichiramba zviri mu supernatant. Kupatsanurwa uku kunoitika nekuda kwekusiyana kwehuwandu hwezvidimbu, chimiro, saizi, uye kudyidzana kwazvo nechinhu chakakomberedza.

Mhando dzeCentrifuges uye Mashandisirwo Adzo

Kune mhando dzakasiyana dzema "centrifuge" dziripo, dzinosanganisira ma "ultracentrifuge", ma "microcentrifuge", uye ma "high-speed centrifuge", imwe neimwe yakagadzirirwa kuzadzisa zvinodiwa zvekuparadzanisa. Semuenzaniso, ma "Ultracentrifuge" anokwanisa kugadzira masimba makuru e "centrifugal", zvichiita kuti zvidimbu zviparadzaniswe mu "nanometer range". Kusiyana neizvi, ma "microcentrifuge" madiki uye anoshandiswa zvakanyanya mumarabhoritari ekiriniki ekuparadzanisa sampuli nguva dzose.

Nzira dzekuparadzanisa dzinoshandiswa muCentrifugation

Kuparadzanisa kunoshandisa nzira dzakasiyana-siyana, zvichienderana nemhando yemuenzaniso uye mhedzisiro inodiwa. Nzira idzi dzinosanganisira differential centrifugation, rate-zonal centrifugation, density gradient centrifugation, uye isopycnic centrifugation.

Kuparadzanisa zvidimbu zvinokwanisa kupatsanurana zvichienderana nehukuru hwazvo uye kuwanda kwazvo. Nekuisa musanganiswa uyu pamatanho akatevedzana ekuparadzanisa zvidimbu zvinofamba nekumhanya kwakawedzera, zvidimbu zvidiki zvinotanga zvadonha, zvichiteverwa nezvidimbu zvikuru, zvichikonzera kupatsanurana.

Kubatanidza masero munzvimbo dzakasiyana-siyana, kune rumwe rutivi, kunosanganisira kurodha sampuli panzvimbo ine mativi akasiyana-siyana. Kubatanidza masero kunobva kwaita kuti zvidimbu zvifambe nepakati pemagiraidhi zvichienderana nehukuru hwavo nechimiro, zvichizopedzisira zvagadzira mabhandi akasiyana.

Kushandiswa kwe density gradient centrifugation kunoshandisa density gradients inogadzirwa nezvinhu zvakaita se sucrose kana cesium chloride. Zvidimbu zvinonyura ne gradient kusvika zvasvika panzvimbo yakaenzana, zvichikonzera kuparadzaniswa. Nzira iyi inonyanya kubatsira pakuparadzanisa organelles kana marudzi akasiyana emasero.

Mukushandiswa kwe isopycnic centrifugation, zvidimbu zvinoparadzaniswa zvichienderana nehuwandu hwazvo hwekuyerera. Nekuisa sampuro pa gradient apo zvidimbu zvine huwandu hwakafanana nechinhu chakakomberedza, zvidimbu zvinoyangarara kana kunyura kusvika zvanyatsoenzana. Nzira iyi inoshandisa DNA neRNA isolation.

Zvinhu Zvinokanganisa Kushanda Zvakanaka kweCentrifugation

Zvinhu zvakawanda zvinopesvedzera kushanda zvakanaka uye kushanda zvakanaka kwe centrifuge. Izvi zvinosanganisira kumhanya kwenguva nenguva ye centrifuge, tembiricha, rudzi rwe rotor nehukuru, huwandu hwemuenzaniso, uye sarudzo yenzira yekuparadzanisa. Kugadzirisa izvi zvakakosha kuti uwane mhedzisiro chaiyo uye inoenderana yekuparadzanisa.

Mashandisirwo eCentrifugation muMinda Yakasiyana-siyana

Kuchenesa ropa kunoshandiswa mu "centrifugation" kunoshandiswa zvakasiyana-siyana munzvimbo dzakasiyana. Mumarabhoritari ekurapa, kunoshandiswa pakupatsanura ropa, zvichiita kuti plasma kana serum zvikwanise kupatsanurwa. Muindasitiri yemishonga, kuchenesa ropa kunobatsira mukuchenesa, kuparadzanisa zvinhu zvinoshanda kubva mutsvina. Tsvagiridzo yezvakatipoteredza inoshandisa kuvheneka ropa mukuongorora masampuro etsvina, zvichibvumira kuzivikanwa kwezvinosvibisa netupukanana.

Mukupedzisa, centrifugation inoita basa rakakosha mukuita kwekuparadzanisa, ichipa chishandiso chine simba kune vaongorori nemaindasitiri muzvikamu zvakasiyana-siyana. Kunzwisisa misimboti, mhando, uye nzira dze centrifugation, pamwe nekugadzirisa zvinhu zvakakosha, kunogona kutungamira mukuparadzanisa kwakanaka uye kupa mhedzisiro chaiyo. Nekushandisa kugona kwe centrifugation, masayendisiti anoramba achiburitsa mikana mitsva mumunda wesainzi yekuparadzanisa.