me pēhea te wehewehe mā te centrifugation
He Kupu Whakataki ki te Whakapūtanga me tōna Hiranga i roto i ngā Tukanga Wehewehe
He tikanga whānui te whakamahi i te centrifugation i roto i ngā ahumahi me ngā mara rangahau hei wehewehe i ngā ranunga kanorau i runga i te rahi me te mātotoru o ngā matūriki. Ahakoa i roto i ngā taiwhanga hauora, i ngā ahumahi rongoā, i ngā rangahau taiao rānei, kāore e taea te whakanui rawa i te hiranga o te centrifugation hei whakatutuki i te wehenga whai hua.
Ko te whāinga o tēnei tuhinga he whakarato i tētahi aratohu whānui mō te wehewehe whai hua i ngā matū mā te whakamahi i te centrifugation. Ka matapakihia e mātou te mātāpono o te centrifugation, ka tūhuratia ngā momo centrifuges rerekē, ka rukuhia ngā tikanga wehewehe rerekē, ka whakamāramahia ngā āhuatanga matua e pā ana ki te pai o te wehenga, ā, ka mutu me ētahi whakamahinga o tēnei tikanga.
Te Mārama ki te Mātāpono o te Whakapūtanga
Ko te mahi a te centrifugation i runga i te mātāpono o te whakatōpuku, arā, mā te kaha centrifugation ka neke ngā matūriki matotoru ki raro, ka hanga he pirepire, ko ngā matūriki māmā ake ka noho tonu ki roto i te wai. Ka tutuki tēnei wehenga nā te rerekētanga o te matotoru o te matūriki, te āhua, te rahi, me tā rātou taunekeneke ki te wai e karapoti ana.
Ngā Momo o ngā Centrifuge me ā rātou Whakamahinga
He rerekē ngā momo pūwhakatere e wātea ana, tae atu ki ngā pūwhakatere tino-rahi, ngā pūwhakatere iti, me ngā pūwhakatere tere-rahi, ia mea i hangaia hei whakatutuki i ngā whakaritenga wehewehe motuhake. Hei tauira, ka taea e ngā pūwhakatere tino-rahi te whakaputa i ngā kaha whakatere tino teitei, e āhei ai te wehenga o ngā matūriki i roto i te awhe nanometa. I tetahi atu taha, he kikī ngā pūwhakatere iti, ā, he mea whakamahi noa i roto i ngā taiwhanga haumanu mō te wehenga tauira noa.
Ngā Tikanga Wehewehe e Whakamahia ana i roto i te Centrifugation
He maha ngā tikanga wehewehe e whakamahia ana i roto i te centrifugation, i runga i te āhua o te tauira me te putanga e hiahiatia ana. Kei roto i ēnei tikanga ko te centrifugation rerekē, te centrifugation tere-rohe, te centrifugation pikinga mātotoru, me te centrifugation isopycnic.
Mā te whakamahi i te centrifugation rerekē ka taea te wehewehe i ngā matūriki i runga i te rahi me te mātotoru. Mā te tuku i te ranunga ki ngā huringa centrifugation karapīpiti i ngā tere piki haere, ka pupū tuatahi ngā matūriki iti, ā, muri iho ka pupū ngā matūriki nunui, ka mutu ka wehea.
Ko te whakaterenga tere-rohe, i tētahi atu taha, ko te uta i te tauira ki runga i tētahi reo pikinga o te mātotoru rerekē. Kātahi ka meinga e te whakaterenga kia heke ngā matūriki i roto i te pikinga i runga i tō rātou rahi me te āhua, ā, ka mutu ka hangaia he roopu motuhake.
Ka whakamahia e te centrifugation gradient mātotoru ngā gradient mātotoru i hangaia e ngā matū pēnei i te sucrose, te cesium chloride rānei. Ka whakatōngia ngā matūriki mā roto i te gradient kia tae rā anō ki tētahi rohe o te mātotoru ōrite, ka mutu ka wehea. He tino whai hua tēnei tikanga ina wehea ngā organelles, ngā momo pūtau rerekē rānei.
I roto i te centrifugation isopycnic, ka wehea ngā matūriki i runga i tō rātou kiato pōkarekare. Mā te uta i te tauira ki runga i tētahi taumata e rite ana te kiato o ngā matūriki ki te reo e karapoti ana, ka mānu, ka totohu rānei ngā matūriki kia tae rā anō ki te taurite. Ka kitea ngā whakamahinga o tēnei tikanga i roto i te wehenga DNA me te RNA.
Ngā Take e Pā Ana ki te Whai Hua o te Whakapūtanga
He maha ngā āhuatanga e pā ana ki te whai huatanga me te whaihua o te centrifugation. Kei roto i ēnei ko te tere me te wā centrifuge, te pāmahana, te momo me te rahi o te rotor, te rahi o te tauira, me te kōwhiringa o te tikanga wehewehe. He mea nui te arotau i ēnei taurangi kia tika ai, kia ōrite ai hoki ngā hua wehewehe.
Ngā Whakamahinga o te Centrifugation i ngā Marae Rerekē
He whānuitia ngā whakamahinga o te centrifugation puta noa i ngā mara rerekē. I roto i ngā taiwhanga hauora, ka whakamahia mō te wehenga tauira toto, ka taea ai te wehe i te plasma, i te serum rānei mō ngā take tātaritanga. I roto i te umanga rongoā, ka āwhina te centrifugation i ngā tukanga purenga, ka wehe i ngā kai hohe mai i ngā poke. Ka whakamahia e te rangahau taiao te centrifugation hei tātari i ngā tauira paru, ka taea ai te tautuhi i ngā poke me ngā moroiti.
Hei whakamutunga, he mea nui te mahi a te centrifugation i roto i ngā tukanga wehewehe, he taputapu kaha hoki mō ngā kairangahau me ngā ahumahi puta noa i ngā rāngai rerekē. Mā te mārama ki ngā mātāpono, ngā momo, me ngā tikanga o te centrifugation, me te arotau i ngā āhuatanga matua, ka taea te arahi ki te wehewehenga whai hua me te whakaputa hua tika. Mā te whakamahi i te pūmanawa o te centrifugation, ka haere tonu ngā kaipūtaiao ki te wetewete i ngā āheinga hou i roto i te mara o te pūtaiao wehewehe.
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