mokhoa oa ho arola ka centrifugation

Selelekela sa Centrifugation le Bohlokoa ba Eona Mekhoeng ea Karohano

Ho centrifugation ke mokhoa o sebelisoang haholo liindastering tse fapaneng le mafapheng a lipatlisiso bakeng sa ho arola metsoako e sa tšoaneng ho latela boholo ba likaroloana tsa tsona le bongata ba tsona. Ebang ke lilaboratoring tsa bongaka, liindastering tsa meriana, kapa lipatlisisong tsa tikoloho, bohlokoa ba ho centrifugation ho fihlelleng karohano e sebetsang bo ke ke ba feteletsoa.

Sehlooho sena se ikemiseditse ho fana ka tataiso e felletseng mabapi le mokhoa oa ho arola lintho ka katleho ka ho sebelisa centrifugation. Re tla buisana ka molao-motheo oa centrifugation, re hlahlobe mefuta e fapaneng ea centrifuge, re hlahlobe mekhoa e fapaneng ea karohano, re totobatse lintlha tsa bohlokoa tse susumetsang katleho ea karohano, 'me re qetelle ka ts'ebeliso e meng ea mokhoa ona.

Ho utloisisa Molao-motheo oa Centrifugation

Ho centrifugation ho sebetsa hodima molao-motheo wa ho sedimentation, moo tshebediso ya matla a centrifugal e etsang hore dikarolwana tse teteaneng di tsamaye ho ya tlase, di etse pellet, ha dikarolwana tse bobebe di ntse di le ka hara supernatant. Karohano ena e fihlellwa ka lebaka la phapang ya bongata ba dikarolwana, sebopeho, boholo, le tshebedisano ya tsona le sebaka se potolohileng.

Mefuta ea Li-Centrifuge le Litšebeliso tsa Tsona

Ho na le mefuta e fapaneng ea li-centrifuge tse fumanehang, ho kenyeletsoa li-ultracentrifuge, li-microcentrifuge, le li-centrifuge tse lebelo le phahameng, e 'ngoe le e 'ngoe e etselitsoe ho khotsofatsa litlhoko tse itseng tsa karohano. Li-ultracentrifuge, mohlala, li khona ho hlahisa matla a phahameng haholo a centrifuge, e leng se nolofalletsang karohano ea likaroloana tse ka har'a nanometer. Ka lehlakoreng le leng, li-microcentrifuge li nyane 'me li sebelisoa hangata lilaboratoring tsa bongaka bakeng sa karohano ea sampole ea kamehla.

Mekhoa ea ho Arohanya e Sebelisitsoeng ho Centrifugation

Ho centrifugation ho sebedisa mekgwa e fapaneng ya karohano, ho itshetlehile ka mofuta wa sampole le sephetho se lakatsehang. Mekhoa ena e kenyeletsa ho centrifugation e fapaneng, ho centrifugation ya sekgahla-sebaka, ho centrifugation ya gradient ya density, le ho centrifugation ya isopycnic.

Ho arola likaroloana ka tsela e fapaneng ho nolofalletsa karohano ea likaroloana ho latela boholo le boima ba tsona. Ka ho beha motsoako ho potoloha ho latellanang ha ho arola ka lebelo le eketsehileng, likaroloana tse nyane li qala ka ho lahla, ho lateloe ke likaroloana tse kholoanyane, e leng se fellang ka karohano.

Ka lehlakoreng le leng, ho centrifugation ya sekgahla le sebaka, ho kenyelletsa ho kenya sampole hodima sebaka sa gradient se nang le bongata bo fapaneng. Ho centrifugation ebe ho etsa hore dikarolwana di falle ka hara gradient ho latela boholo le sebopeho sa tsona, mme qetellong di thehe dihlopha tse fapaneng.

Ho centrifugation ha gradient e teteaneng ho sebedisa di-gradient tsa density tse hlahisitsweng ke dintho tse kang sucrose kapa cesium chloride. Dikaroloana di tjhesa ka hara gradient ho fihlela di fihla sebakeng se nang le density e lekanang, e leng se lebisang karohanong. Mokgwa ona o thusa haholo ha ho arolwa di-organelle kapa mefuta e fapaneng ya disele.

Ka ho centrifugation ea isopycnic, likaroloana li aroloa ho latela bongata ba tsona bo phaphametseng. Ka ho kenya sampole holim'a gradient moo likaroloana li nang le bongata bo lekanang le sebaka se potolohileng, likaroloana lia phaphamala kapa lia teba ho fihlela tekano e fihlelleha. Mokhoa ona o fumana lits'ebetso ho karohano ea DNA le RNA.

Mabaka a Amang Bokgoni ba ho Centrifugation

Mabaka a 'maloa a susumetsa katleho le katleho ea ho centrifugation. Tsena li kenyelletsa lebelo le nako ea centrifuge, mocheso, mofuta oa rotor le boholo, bophahamo ba sampole, le khetho ea mokhoa oa karohano. Ho ntlafatsa mefuta-futa ena ho bohlokoa bakeng sa ho fihlela liphetho tse nepahetseng le tse tsitsitseng tsa karohano.

Litšebeliso tsa Centrifugation Masimong a Fapaneng

Ho centrifugation ho na le mefuta e mengata ea ts'ebeliso mafapheng a fapaneng. Lilaboratoring tsa bongaka, e sebelisoa bakeng sa karohano ea sampole ea mali, e nolofalletsang ho aroloa ha plasma kapa serum bakeng sa merero ea tlhahlobo. Indastering ea meriana, ho centrifugation ho thusa lits'ebetsong tsa tlhoekiso, ho arola metsoako e sebetsang ho litšila. Lipatlisiso tsa tikoloho li sebelisa ho centrifugation bakeng sa ho sekaseka lisampole tsa sediment, ho lumella ho tsebahatsa litšila le likokoana-hloko.

Qetellong, ho centrifugation ho bapala karolo ea bohlokoa lits'ebetsong tsa karohano, ho fana ka sesebelisoa se matla bakeng sa bafuputsi le liindasteri ho pholletsa le makala a fapaneng. Ho utloisisa melao-motheo, mefuta le mekhoa ea ho centrifugation, hammoho le ho ntlafatsa lintlha tsa bohlokoa, ho ka lebisa karohanong e sebetsang hantle le ho hlahisa liphetho tse nepahetseng. Ka ho sebelisa bokhoni ba ho centrifugation, bo-rasaense ba tsoela pele ho qaqisa menyetla e mecha lefapheng la saense ea karohano.