Ha mali a kenngoa ka setering, a arohana ka likarolo life

Selelekela

Karohano ea mali ke ts'ebetso ea bohlokoa lilaboratoring tsa bongaka le lithutong tse fapaneng tsa lipatlisiso. E kenyelletsa tšebeliso ea centrifuge, sesebelisoa se potolohang lisampole tsa mali ka lebelo le phahameng ho arola likarolo tsa eona ho latela bongata ba tsona. Ha mali a kenngoa centrifuge, a arohana ka likarolo tse fapaneng, e 'ngoe le e 'ngoe e na le litšobotsi le mesebetsi ea eona e ikhethang. Sehloohong sena, re tla hlahloba likarolo tse fapaneng tse thehoang ha mali a etsoa centrifuge le bohlokoa ba tsona tlhahlobong ea bongaka le lipatlisisong.

1. Metheo ea ho Centrifugation ea Mali

2. Ts'ebetso ea Karohano le Tlhahiso ea Likarolo

3. Lisele tse Khubelu tsa Mali: Lijari tsa Oksijene

4. Lisele tse Tšoeu tsa Mali: Basireletsi ba 'Mele

5. Li-platelet: Lintho tse etsang hore ho be le ho ruruha ha mali

6. Plasma: Matrix ea Metsi

7. Bohlokoa ba ho Aroloa ha Mali Litlhahlobong tsa Bongaka

8. Mekhoa e Mecha ea ho Arohanya Mali

9. Karohano ea Mali ka Nģ'ane ho Tlhahlobo

10. Qetello

Metheo ea ho Centrifugation ea Mali

Ho centrifugation ke mokhoa o sebelisoang haholo lipatlisisong tsa bongaka le tlhahlobong ea bongaka. Ka ho arola likarolo ho latela bongata ba tsona, ho lumella bo-rasaense le litsebi tsa tlhokomelo ea bophelo ho ithuta le ho sekaseka likarolo tse fapaneng tsa mali ka katleho. Ts'ebetso ea ho centrifugation e kenyelletsa ho potoloha sampole ea mali ka lebelo le phahameng, e leng se fellang ka matla a centrifugal a etsang hore likarolo tse boima li qhalane ka tlase ho tube.

Mokhoa oa Karohano le Tlhahiso ea Likarolo

Ha mali a kenngoa ka har'a centrifuge, a arohana ka mekhahlelo e meraro e fapaneng: lisele tse khubelu tsa mali (RBC) tlase, lisele tse tšoeu tsa mali (WBC) le li-platelet bohareng, le plasma holimo. Karohano ena e bakoa ke liphapang tsa bongata ba likarolo tsena.

Lisele tse Khubelu tsa Mali: Lijari tsa Oksijene

Lisele tse khubelu tsa mali, tse tsejoang hape e le li-erythrocyte, ke lisele tse ngata ka ho fetisisa maling. Li na le protheine e tsejoang e le hemoglobin, e kopanang le oksijene 'me e e tsamaisa ho pholletsa le' mele. Nakong ea ho centrifugation, li-RBC li etsa pellet e teteaneng tlase ho tube. 'Mala oa tsona o mofubelu o ruileng ke phello ea molek'hule e tsamaisang oksijene. Ho utloisisa palo, boholo le sebōpeho sa li-RBC ho bohlokoa bakeng sa ho hlahloba khaello ea mali, mathata a liphio le maemo a mang a fapaneng.

Lisele tse Tšoeu tsa Mali: Basireletsi ba 'Mele oa ho Sireletsa

Lisele tse tšoeu tsa mali, kapa li-leukocyte, li phetha karolo ea bohlokoa tsamaisong ea 'mele ea ho itšireletsa mafung. Li sireletsa 'mele khahlanong le tšoaetso, likokoana-hloko le lintho tse tsoang linaheng tse ling. Ho fapana le li-RBC, li-WBC li fokola haholo ka palo. Nakong ea ho centrifugation, li theha lera le lesesaane ka holim'a lisele tse khubelu tsa mali. Ho sekaseka mefuta le mahloriso a li-WBC ho fana ka temohisiso ea bohlokoa mabapi le ts'ebetso ea 'mele ea ho itšireletsa mafung' me ho thusa ho fumana tšoaetso, allergy, mathata a autoimmune le leukemia.

Liplatelete: Lintho tse Amanang le ho Khema ha Mafura

Li-platelet, kapa li-thrombocyte, li bohlokoa bakeng sa ho hoama ha mali, li thibela ho tsoa mali ho feteletseng ha kotsi e hlaha. Likaroloana tsena tsa li-platelet li nyane le ho feta ka palo ha li bapisoa le li-RBC le li-WBC empa li ngata maling. Nakong ea ho centrifugation, li-platelet li bokellana ka holim'a lera la lisele tse tšoeu tsa mali. Thuto ea palo ea li-platelet le mosebetsi e ka thusa ho hlahloba mathata a ho tsoa mali, ho beha leihlo mekhoa ea phekolo ea anticoagulant, le ho hlahloba kotsi ea mafu a pelo le methapo ea mali.

Plasma: Matrix ea Metsi

Plasma ke karolo ea mali e 'mala o kang oa lehlaka, e mokelikeli e setseng ka mor'a hore lisele li tlosoe. E etsa hoo e ka bang 55% ea mali 'me e na le metsi, li-electrolyte, liprotheine, lihormone, li-metabolite le lihlahisoa tse litšila. Nakong ea ho centrifugation, plasma e lula karolong e ka holimo ea tube. Ho sekaseka likarolo tsa plasma ho fana ka tlhahisoleseling ea bohlokoa mabapi le bophelo bo botle ba motho ka kakaretso, ho kenyeletsoa mesebetsi ea sebete le liphio, ho leka-lekana ha electrolyte, boemo ba lipid le matšoao a mafu a fapaneng.

Bohlokoa ba ho Aroloa ha Mali Litlhahlobong tsa Bongaka

Ho aroloa ha mali ka ho sebelisa centrifugation ho phetha karolo ea bohlokoa tlhahlobong ea bongaka. E nolofalletsa litsebi tsa tlhokomelo ea bophelo ho sekaseka likarolo ka bonngoe ka nepo le ho khetholla lintho tse sa tloaelehang kapa ho se leka-lekane. Mohlala, ka ho lekanya maemo a lienzyme kapa liprotheine tse itseng plasma, lingaka li ka fumana maemo a kang tlhaselo ea pelo, mafu a sebete, kapa mathata a liphio.

Mekhoa e Mecha ea ho Arohanya Mali

Tsoelo-pele theknolojing e lebisitse nts'etsopele ea mekhoa e mecha ea karohano ea mali. Mokhoa o mong o joalo ke density gradient centrifugation. E kenyelletsa ho beha gradient ea density tse fapaneng ka har'a tube ea centrifuge. Ha sampole e ntse e potoloha, likarolo li tsamaea ka har'a gradient, li arohana ka likarolo tse fapaneng ho latela density tsa tsona. Mokhoa ona o lumella ho aroloa ka ho nepahala le ho feta ha likarolo tse itseng tsa mali bakeng sa lits'ebetso tse fapaneng.

Karohano ea Mali ka Nģ'ane ho Tlhahlobo

Ntle le ho hlahloba bongaka, mekhoa ea ho arola mali e fumana lits'ebetso lipatlisisong tsa bongaka le mekhoa ea phekolo. Bafuputsi ba sebelisa centrifugation ho arola likarolo tse itseng bakeng sa ho ithuta mesebetsi ea tsona, ho etsa liteko tsa lefutso, kapa ho nts'etsapele meriana e mecha. Ho feta moo, mekhoa e meng ea phekolo, joalo ka phekolo ea plasma e ruileng ka platelet (PRP), e sebelisa mekhoa ea ho arola mali ho ntša plasma e ruileng ka platelet le ho e kenya liseleng tse lemetseng bakeng sa pholiso e ntlafalitsoeng.

Qetello

Qetellong, ts'ebetso ea ho arola mali ka seterifugation e nolofalletsa karohano ea likarolo tse fapaneng, e leng lisele tse khubelu tsa mali, lisele tse tšoeu tsa mali, li-platelet le plasma. Karolo ka 'ngoe e bapala karolo ea bohlokoa ts'ebetsong ea' mele 'me e fana ka temohisiso ea bohlokoa mabapi le bophelo bo botle ba motho. Ka ho utloisisa litšobotsi le mesebetsi e ikhethang ea likarolo tsena, litsebi tsa tlhokomelo ea bophelo li ka hlahloba mafu, tsa beha leihlo mekhoa ea phekolo, le ho etsa lipatlisiso tsa bohlokoa ho ntšetsa pele tsebo ea bongaka le ho ntlafatsa tlhokomelo ea bakuli.