mhando ipi yemisanganiswa inogona kuparadzaniswa ne centrifugation

1. Nhanganyaya yeCentrifugation neMashandisirwo ayo

2. Kuparadzanisa Misanganiswa Yakafanana Uchishandisa Centrifugation

3. Maitiro ekugadzira centrifugation yemisanganiswa yakasiyana-siyana

4. Kuisa Centrifugation muMaindasitiri: Kubva kuMishonga kusvika kuKugadzira Chikafu

5. Kufambira Mberi muTekinoroji yeCentrifugation

Nhanganyaya yeCentrifugation uye Mashandisirwo Ayo

Kutenderedza zvinhu (centrifugation) inzira inoshandiswa zvakanyanya mu sainzi neindasitiri pakupatsanura zvikamu zvakasiyana zvemusanganiswa zvichienderana nekuwanda kwazvo. Nekutenderedza sampuro nekukurumidza nekumhanya kwakanyanya, kutenderedza zvinhu (centrifugation) kunoshandisa misimboti yegiravhiti nesimba repakati (centrifugation) kupatsanura musanganiswa kuita zvikamu zvakasiyana. Kubva pakucheneswa kwemasampuro ehupenyu kusvika pakupatsanurwa kwezvigadzirwa zvakasiyana-siyana zveindasitiri, kutenderedza zvinhu (centrifugation) kwaratidza kuva chishandiso chakakosha muminda yakawanda.

Kuparadzanisa Misanganiswa Yakafanana Uchishandisa Centrifugation

Misanganiswa yakafanana, umo zvikamu zvacho zvakagoverwa zvakaenzana, inoda nzira dzakatarwa dze centrifugation kuti zviparadzaniswe. Imwe nzira inowanzoshandiswa ndeye differential centrifugation, iyo inoshandisa matanho akawanda e centrifugation nekumhanya kwakasiyana kuti iparadzanise zvidimbu zvichienderana nehukuru hwazvo uye huwandu hwazvo. Nekuwedzera kumhanya zvishoma nezvishoma, zvidimbu zvidiki zvinotanga zvadonha, zvichisiya zvidimbu zvikuru pamusoro. Iyi nzira inowanzoshandiswa mukutsvagisa kwemakemikari kuparadzanisa organelles kubva kumasero kana zvikamu zvakasiyana zvemasero.

Kune rumwe rutivi, density gradient centrifugation inoparadzanisa zvidimbu zvichibva pakuwanda kwazvo. Nekugadzira concentration gradient uchishandisa denser solution (yakadai sesucrose) pasi pezvidimbu zvakareruka, sampuro inoiswa pamusoro payo. Kana yaiswa centrifuge, zvidimbu zvinofamba nepakati pe gradient kusvika zvasvika pakuenzana panzvimbo yakatarwa ne density yazvo. Iyi nzira inobvumira kupatsanurwa kwe nucleic acids, mapuroteni, nezvimwe zvikamu zviripo mu biological samples.

Maitiro eCentrifugation eMisanganiswa Yakasiyana-siyana

Misanganiswa yakasiyana-siyana, ine zvikamu zvakasiyana zvinooneka, inogona kupatsanurwa uchishandisa nzira dzakasiyana dze centrifugation. Sedimentation centrifugation inowanzo shandiswa pamisanganiswa yakadaro. Sezvinoratidzwa nezita racho, nzira iyi inosanganisira kunyungudutsa zvidimbu zvichienderana nekuwanda kwazvo. Zvikamu zvinorema zvinogara pasi pechubhu ye centrifuge nepo zvikamu zvakareruka zvichiramba zviri mu supernatant.

Mune zvimwe zviitiko, misanganiswa yakasiyana-siyana inogona kupatsanurwa uchishandisa nzira inonzi filtration centrifugation kana screen centrifugation. Iyi nzira inoshandisa firita medium, senge sefa, kuchengetedza zvidimbu zvikuru uku ichibvumira zvidimbu zvidiki kuti zvipfuure nepakati. Nekushandisa musiyano wehukuru hwezvidimbu, filtration centrifugation inobudirira kupatsanura zvikamu zvichienderana nehukuru hwazvo. Iyi nzira yakawana mashandisirwo mukurapa mvura yakasviba, kugadzira mishonga, uye mamwe maindasitiri akasiyana-siyana.

Kuchengetedza nharaunda munzvimbo dzemaindasitiri: Kubva kuMishonga kusvika pakugadzira chikafu

Kugadzira mishonga ye "centrifugation" kunoita basa guru mumaindasitiri akasiyana-siyana, anosanganisira mishonga yekurapa, biotechnology, uye kugadzira chikafu. Muchikamu chemishonga, kugadzira mishonga ye "centrifugation" kunowanzo shandiswa mukutsvaga mishonga, kugadzira majekiseni, uye kupatsanura zvinhu zvinoshanda. Kunobatsira kuchenesa mishonga ye "compounds" nekubvisa tsvina, zvichiita kuti mishonga ibude yakachengeteka uye inoshanda.

Muchikamu che biotechnology, centrifugation yakakosha muDNA sequencing, kuchenesa mapuroteni, uye kushandiswa kwe cellular culture. Nekuparadzanisa zvikamu zvakasiyana zvemasero, centrifugation inobvumira vaongorori kudzidza nekuongorora ma biomolecule chaiwo, zvichibatsira munzvimbo dzakadai sekutsvagisa majini nekugadzirwa kwemishonga.

Muindasitiri yekugadzira chikafu, centrifugation inoshandiswa kujekesa, kuchenesa, uye kuisa mvura muhuwandu. Inoshandiswa zvakanyanya mukugadzira muto wemichero, mafuta anodyiwa, zvigadzirwa zvemukaka, uye zvinwiwa zvinodhaka. Centrifuges inobatsira kubvisa zvidimbu zvisingadiwi, kuparadzanisa mvura kubva kune yakasimba, uye kuwedzera kunaka kwechigadzirwa chekupedzisira.

Kufambira Mberi muTekinoroji yeCentrifugation

Mumakore apfuura, tekinoroji ye centrifugation yafambira mberi zvakanyanya, zvichikonzera mhedzisiro yekuparadzanisa inoshanda uye chaiyo. Kuunzwa kwe centrifuges dzinomhanya zvakanyanya uye dzine simba guru kwakachinja munda uyu, zvichigonesa vaongorori kugadzirisa huwandu hwakawanda hwesampuru munguva pfupi.

Pamusoro pezvo, kufambira mberi mukugadzirwa kwecentrifuge kwakaita kuti pave nekugadzirwa kwemasystem akasarudzika ekushandisa mamwe mashandisirwo. Semuenzaniso, microcentrifuges yakagadzirirwa huwandu hudiki hwesampuro, nepo ultracentrifuges ichikwanisa kusvika kumhanya kwakanyanya, zvichiita kuti zvive nyore kupatsanurana kwezvikamu zve subcellular uye macromolecules.

Kugadzira ma "centrifuge" kwakabatsirwawo nekufambira mberi mu "otomatiki" uye "control systems", zvichibvumira kuti zvive nyore uye zvinokwanisika kudzokororwa. Ma "centrifuge" emazuva ano ane ma "interfaces" ari nyore kushandisa, ma "programmable settings", uye ma "safety features", zvichiita kuti akwanise kushandiswa nevashandisi vakasiyana-siyana mumaindasitiri akasiyana-siyana.

Mukupedzisa, centrifugation inzira inoshandiswa zvakasiyana-siyana yekuparadzanisa misanganiswa zvichienderana nekuwanda kwayo. Kubva pakuparadzanisa misanganiswa yakafanana uchishandisa differential uye density gradient centrifugation kusvika pakuparadzanisa misanganiswa yakasiyana-siyana uchishandisa sedimentation uye filtration centrifugation, nzira iyi yave kushandiswa zvakanyanya muminda yesainzi neyemaindasitiri. Nekufambira mberi kuri kuitwa mukugadzira centrifuge netekinoroji, remangwana re centrifugation rine mukana mukuru wekuwedzera kugona kwekuparadzanisa uye kuwedzera kugona kwekubudirira.