chii chinonzi nzira yekuparadzanisa centrifugation

Nzira Yekuparadzanisa Mienzaniso Yekuparadzanisa: Gwaro Rakazara Rekuparadzanisa Muenzaniso Unoshanda

Nhanganyaya yeCentrifugation Separation Technique

Kugadzira centrifugation inzira inoshandiswa zvakanyanya muzvikamu zvakasiyana-siyana zvesainzi nekiriniki kupatsanura nekuongorora musanganiswa wakaoma wezvinhu. Inosanganisira kushandisa simba re centrifugal, iro riri simba remakanika rine simba, kupatsanura zvikamu zvakasiyana zvemusanganiswa wakasiyana zvichienderana nehuwandu hwazvo nehukuru hwazvo.

Mhando dzeCentrifugation

Kune mhando mbiri huru dze centrifugation: differential centrifugation uye density gradient centrifugation. Differential centrifugation inoshandiswa kupatsanura zvidimbu zvichienderana nehukuru hwazvo, nepo density gradient centrifugation ichipatsanura zvidimbu zvichienderana nehuwandu hwazvo.

Mukuita kuti centrifugation ive yakasiyana-siyana, sampuro inotenderedzwa nekumhanya kwakanyanya, zvichiita kuti zvidimbu zvakakura, zvinorema zviite sediment nekukurumidza kupfuura zvidimbu zvidiki, zvakareruka. Nekugadzirisa kumhanya nenguva yecentrifugation, zvinokwanisika kuunganidza zvidimbu zvakananga zvine zvidimbu zvinodiwa.

Kushandiswa kwe density gradient centrifugation kunosanganisira kugadzirwa kwe density gradient mukati me sample tube. Density solution inoiswa pamusoro pe density solution isina density, zvichiita gradient. Kana sample yaiswa centrifuge, zvidimbu zvinofamba nepakati pe gradient, zvichigara panzvimbo dzakasiyana zvichienderana ne density yazvo. Iyi nzira inobvumira kupatsanurwa kukuru kwezvidimbu zvine saizi dzakafanana.

Mashandisirwo eCentrifugation

Kuchengetedza masero (centrifugation) kunoshandiswa muzvikamu zvakasiyana-siyana zvesainzi, zvinosanganisira biology, chemistry, mishonga, uye sainzi yezvakatipoteredza. Kunoshandiswa pazvinangwa zvakasiyana-siyana zvakaita sekupatsanurwa kwemasero, kuchenesa mapuroteni, kuwanda kwehutachiona, kubviswa kweDNA, uye kupatsanurwa kwezvikamu.

Kupatsanurwa kwemasero inzira inowanzo shandiswa mukutsvagurudza kwezvipenyu, uko mhando dzemasero dzinofanira kupatsanurwa kubva mumusanganiswa wakaoma. Nekushandisa differential centrifugation, zvinokwanisika kupatsanura masero kubva mumatishu kana ma primary cultures zvichienderana ne sedimentation rates dzawo dzakasiyana.

Kuchenesa mapuroteni ndeimwe nzira inokosha yekuchenesa mapuroteni. Mapuroteni anowanzo patsanurwa kubva mumasero asina kucheneswa nekubvisa mapuroteni akasiyana-siyana zvichiteverwa nenzira dzakasiyana dzekuchenesa mapuroteni. Kuchenesa mapuroteni kunogona kupatsanura mapuroteni ane saizi dzakasiyana, maumbirwo, kana huwandu hwakasiyana.

Mukuongorora utachiona, centrifugation inoshandiswa kusanganisa mavhairasi kubva musampuli dzakaoma. Mavhairasi anobviswa nekukurumidzira centrifugation, zvichibvumira kuongororwa kwawo kana kupararira mumasero.

Zvinhu Zvinokanganisa Kushanda Zvakanaka kweCentrifugation

Zvinhu zvakawanda zvinopesvedzera kushanda zvakanaka uye kushanda zvakanaka kwekupatsanurwa kwe centrifugation. Simba rekumhanya kana centrifugal, rudzi rwe rotor, tembiricha, uye huwandu hwemuenzaniso zvinhu zvakakosha zvinofanirwa kutariswa nekungwarira.

Simba rekumhanya kana kuti rekutenderera rinoona mwero wekudzika kwezvikamu. Kutenderera kwesimba repamusoro kunoshandiswa pakuparadzanisa nekukurumidza, nepo kutenderera kwesimba repasi kuchibvumira kuparadzanisa kwakasimba uye kunononoka. Rudzi rwerotor, ingave yakagadzika angle kana kuti inotenderera, inokanganisawo mashandiro ekuparadzanisa.

Kupisa chimwe chinhu chakakosha chekufunga nezvacho. Kuisa centrifugation pakupisa kwakanyanya kunogona kukonzera kuora kana kukuvara kwemasampuli anonzwa kupisa, nepo tembiricha yakaderera ichigona kuchengetedza kunaka kwemuenzaniso. Kusarudza tembiricha yakakodzera kwakakosha zvichienderana nerudzi rwemuenzaniso.

Huwandu hwemuenzaniso hunogona kukanganisa zvikuru maitiro ekuparadzanisa. Kuwanda kwemuenzaniso kunogona kukonzera kusapatsanurwa zvakanaka kana kukonzera kuvharika kwemachubhu e centrifuge. Zvakakosha kuti vhoriyamu yemuenzaniso ienderane nemamiriro akakodzera e centrifuge kuti uwane mhedzisiro yakanaka.

Kufambira Mberi muTekinoroji yeCentrifugation

Sezvo tekinoroji ichiramba ichifambira mberi, matekiniki ekushandisa centrifugation akaonawo shanduko huru. Kuvandudzwa kwema microcentrifuges nema ultracentrifuges kwakachinja kupatsanurwa kwemasampuro, zvichibvumira kupatsanurwa kunoshanda uye nekukurumidza.

Microcentrifuges inogonesa vaongorori kugadzirisa vhoriyamu diki dzesampuro nekukurumidza uye nguva yekudzikamisa mafambiro. Izvi zvinonyanya kubatsira kune mashandisirwo anoda vhoriyamu diki dzesampuro kana pakushanda nemasampuro anokosha kana mashoma.

Kune rumwe rutivi, ma "Ultracentrifuges" anogona kugadzira masimba makuru e "centrifugal", zvichiita kuti pave nekupatsanurwa kwepamusoro kwezvikamu zvine huwandu hwakasiyana. Anoshandiswa zvakanyanya munzvimbo dzekutsvagisa nemarabhoritari pakushandisa zvinoda kunyatsojeka uye kugadziriswa kwakanyanya.

Kuwedzera kune hardware yakagadziridzwa, kufambira mberi mumagadzirirwo erotor, akadai se fixed-angle, swinging-bucket, kana vertical rotors, kwakavandudza kugona uye kushanduka-shanduka kwehunyanzvi hwe centrifugation.

Mhedziso:

Kupinza zvinhu mu centrifugation inzira yakakosha yekuparadzanisa nekuongorora misanganiswa yakaoma munzvimbo dzakasiyana dzesainzi nedzekiriniki. Ingava yekuparadzanisa masero, kuchenesa mapuroteni, kuisa mavhairasi mu centrifugation, kana kuparadzanisa zvidimbu, kupinza zvinhu mu centrifugation kunopa nzira yakavimbika uye inoshanda yekuparadzanisa sampuli. Nekufambira mberi kuri kuitika mu tekinoroji, matekiniki ekubatanidza zvinhu ari kuramba achichinja, zvichipa masayendisiti maturusi ari nani ekutsvaga kwavo uye zvinodiwa pakuongorora.