he aha te tikanga wehenga centrifugation
Tikanga Wehewehenga Pūwero: He Aratohu Whānui ki te Wehewehenga Tauira Whai Hua
He Kupu Whakataki ki te Tikanga Wehewehenga Pūwero
He tikanga whānui te whakamahi i te centrifugation i roto i ngā momo mara pūtaiao me ngā mara haumanu hei wehewehe me te tātari i ngā ranunga matatini o ngā matū. Kei roto i tēnei ko te whakamahinga o te kaha centrifugal, he kaha miihini kaha, hei wehewehe i ngā wāhanga rerekē o te ranunga kanorau i runga i tō rātou kiato me te rahi.
Ngā momo o te centrifugation
E rua ngā momo matua o te centrifugation: ko te centrifugation rerekētanga me te centrifugation pikinga mātotoru. Ka whakamahia te centrifugation rerekētanga hei wehewehe i ngā matūriki i runga i tō rātou rahi, ko te centrifugation pikinga mātotoru ia ka wehewehe i ngā matūriki i runga i tō rātou mātotoru.
I roto i te whakapūtanga rerekē, ka mirohia te tauira i ngā tere teitei, ka tere ake te putunga o ngā matūriki nui ake, taumaha ake i ngā matūriki iti ake, māmā ake. Mā te whakarerekē i te tere me te wā o te whakapūtanga, ka taea te kohikohi i ngā wāhanga motuhake kei roto ngā matūriki e hiahiatia ana.
Ko te whakapūtanga i te pikinga matotoru ko te waihanga i tētahi pikinga matotoru i roto i te ngongo tauira. Ka whakatakotoria he wairewa matotoru ki runga i tētahi wairewa iti ake te matotoru, ka hanga he pikinga. Ina whakapūtangahia te tauira, ka heke ngā matūriki mā roto i te pikinga, ka tau ki ngā tūranga rerekē i runga i tō rātou matotoru. Mā tēnei tikanga ka taea te wehewehe nui ake i ngā matūriki he rite te rahi.
Ngā Whakamahinga o te Whakapūtanga
Ka kitea ngā whakamahinga o te centrifugation i roto i ngā momo mara pūtaiao, tae atu ki te koiora, te matū, te rongoā, me te pūtaiao taiao. Ka whakamahia mō ngā kaupapa rerekē pērā i te wehenga pūtau, te purenga pūmua, te kukū huaketo, te tango DNA, me te wehenga matūriki.
He whakamahinga noa te wehewehe pūtau i roto i te rangahau koiora, ina hiahiatia he momo pūtau motuhake mai i tētahi ranunga matatini. Mā te whakamahi i te centrifugation rerekē, ka taea te wehewehe i ngā pūtau mai i ngā kiko, i ngā ahurea tuatahi rānei i runga i ō rātou tere whakatōpuku rerekē.
Ko te purenga pūmua tētahi atu whakamahinga nui o te centrifugation. He maha ngā wā ka wehea ngā pūmua mai i ngā tangohanga pūtau mata mā te centrifugation rerekē, ā, muri iho ka whai ake ko ngā tikanga purenga motuhake. Ka taea e te centrifugation te wehewehe pai i ngā pūmua he rerekē te rahi, te āhua, te mātotoru rānei.
I roto i te pūtaiao huaketo, ka whakamahia te centrifugation hei whakaemi i ngā huaketo mai i ngā tauira uaua. Ka whakapūpūhia ngā huaketo mā te centrifugation tere-teitei, kia taea ai te tātari, te horapa rānei i roto i ngā ahurea pūtau.
Ngā Take e Pā Ana ki te Whai Hua o te Whakapūtanga
He maha ngā āhuatanga e pā ana ki te whai huatanga me te whaihua o te wehenga centrifugation. Ko te tere, te kaha centrifugation rānei, te momo rotor, te pāmahana, me te rahinga tauira he tawhā nui me āta whakaaroaro.
Ko te tere, te kaha rānei o te porotītaha e whakatau ana i te tere o te whakatōtō o ngā matūriki. Ka whakamahia te porotītaha tere-tiketike mō ngā wehenga tere, ko te porotītaha tere-iti ia ka taea te wehe ngāwari me te puhoi. Ka pā anō hoki te momo rotor, ahakoa he koki-pūmau, he peere-pōturi rānei, ki te mahi wehenga.
Ko te pāmahana tētahi atu mea nui hei whakaaroaro. Tērā pea ka pakaru, ka pakaru rānei ngā tauira tairongo-wera i te wā e whakamahia ana te pūwero i ngā pāmahana teitei ake, engari ka taea e ngā pāmahana iti te pupuri i te kounga o te tauira. Me āta whiriwhiri i te pāmahana tika i runga i te momo tauira.
Ka pā nui te rahinga o te tauira ki te tukanga wehenga. Mā te nui rawa o te tauira ka kore e whai hua te wehenga, ka ārai rānei i ngā ngongo centrifuge. He mea nui kia taurite te rahinga o te tauira me ngā tikanga centrifuge tika kia eke ai ki ngā hua tino pai.
Ngā Whakamuatanga i roto i te Hangarau Whakapūmau
I te haere tonu o te whanaketanga o te hangarau, kua kitea hoki ngā whakapainga nui o ngā tikanga centrifugation. Nā te whanaketanga o ngā microcentrifuges me ngā ultracentrifuges kua tino hurihia te wehenga tauira, kia taea ai te wehenga tere me te whai hua ake.
Mā ngā microcentrifuges ka taea e ngā kairangahau te tukatuka i ngā rahinga tauira iti ake me ngā wā whakatere me ngā wā whakaheke tere. He tino whai hua tēnei mō ngā tono e hiahia ana ki ngā rahinga tauira iti, i te wā rānei e mahi ana me ngā tauira whai hua, iti rānei.
I tētahi atu taha, ka taea e ngā pūwhakawhitiwhiti ultra-whakaputa kaha teitei te whakaputa, ka tutuki ai te wehenga pai rawa atu o ngā matūriki me ngā matotoru rerekē. He whānuitia te whakamahinga i roto i ngā wāhi rangahau me ngā taiwhanga mō ngā tono e hiahia ana ki te tino tika me te whakatau.
Haunga ngā taputapu kua whakapaitia ake, kua whakarei ake ngā whanaketanga o ngā hoahoa rotor, pērā i ngā rotor koki-pūmau, ngā rotor-swinging, ngā rotor poutū rānei, i ngā āheinga me ngā whānui o ngā tikanga centrifugation.
Whakatau:
He tikanga nui te centrifugation mō te wehewehe me te tātari i ngā ranunga matatini i roto i ngā mara pūtaiao me ngā mara haumanu. Ahakoa hei wehe i ngā pūtau, hei pure i ngā pūmua, hei whakaemi i ngā huaketo, hei wehewehe rānei i ngā matūriki, ka tukuna e te centrifugation he tikanga pono me te whai hua mō te wehenga tauira. I te haere tonu o ngā whanaketanga hangarau, kei te whanake tonu ngā tikanga centrifugation, e whakarato ana i ngā taputapu pai ake ki ngā kaipūtaiao mō ā rātou hiahia rangahau me te tātari.
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