Ndeipi miganhu yekupatsanurwa nekuparadzaniswa ne centrifugation?

Ndeipi Miganhu Yekupatsanurana uye Kupatsanurwa neCentrifugation?

Nhanganyaya:

Kupinza zvinhu mu "centrifugation" inzira inoshandiswa zvakanyanya murabhoritari yekuparadzanisa nekupatsanura zvikamu zvakasiyana zvemusanganiswa zvichibva pakuwanda kwazvo uchishandisa musimboti we "sedimentation". Izvi zvinosanganisira kutenderera nekukurumidza kwemuenzaniso nekumhanya kwakanyanya, zvichiita kuti zvidimbu kana zvikamu zvigare pasi nepo zvidimbu zvakareruka zvichiramba zviri pamusoro. Kunyange zvazvo kupinza zvinhu mu "centrifugation" chiri chishandiso chine simba, chine miganhu yacho. Muchinyorwa chino, tichaongorora miganhu yekupatsanura nekupatsanura ne "centrifugation", tichiratidza mamwe matambudziko makuru anosangana nevaongorori pavanenge vachishandisa nzira iyi.

1. Hukuru hwezvikamu uye Kuwanda kwazvo:

Chimwe chezvinhu zvakakosha zvinosarudza kubudirira kwekupatsanurwa nekupatsanurwa ne centrifugation ihukuru hwe particles uye density. Centrifugation inoshanda zvikuru pakupatsanura particles dzine musiyano mukuru mu density, dzakadai se organelles, macromolecules, kana masero madiki. Zvisinei, kana particles kana zvikamu zvine densities kana saizi dzakafanana, kupatsanurwa kwakakodzera kunova kwakaoma. Simba re centrifugal rinogona kusakwana kuti pave nekupatsanurwa kwakakwana, zvichikonzera kupindirana kwema bands kana kusakwana kwekupatsanurwa.

2. Kuchera netsvina:

Kusanganiswa kwemvura nesimbi zvinoreva dambudziko rekuti zvimwe zvikamu kana zvidimbu zvinoungana pamwe chete panguva yekubatanidza simbi, zvichiita kuti zviome kuzviparadzanisa zvachose. Chiitiko ichi chinoitika kana musiyano wehuwandu hwezvinhu uri mudiki, zvichikonzera kufamba pamwe chete panguva yekubatanidza simbi nesimbi. Kusanganiswa kwemvura nesimbi kunogona kuva dambudziko guru pakushanda nemisanganiswa yakaoma, yakadai semasamples ehupenyu kana macromolecular complexes, uko kupatsanurwa kwezvikamu zvakati kwakaoma nekuda kwehunhu hwazvo hwakafanana.

3. Muenzaniso weKusvibiswa:

Munguva yekuisa centrifugation, kusvibiswa kwesampuli kunogona kuva dambudziko guru. Zvakakosha kuve nechokwadi chekuti mamiriro ekuisa centrifugation uye ma parameters zvakagadziridzwa kuderedza kusvibiswa kubva kumachubhu e rotor, machubhu, kana ekuunganidza. Zvinosvibisa zvinogona kukanganisa maitiro ekuparadzanisa, zvichikonzera masampuli asina kuchena. Kunyatsoteerera kunofanirwa kubhadharwa pakusarudza zvinhu zvakakodzera zve rotor, kushandisa machubhu ekuisa centrifugation akakodzera, uye kushandisa nzira dzekuchenesa dzinoshanda kudzivirira kusvibiswa kwesampuli.

4. Kugadziriswa Kushoma:

Kugadziriswa kwekupatsanurwa nekupatsanurwa ne centrifugation kunonyanya kuenderana nesarudzo ye centrifuge ne rotor. Nzira dzakasiyana dze centrifugation, dzakadai se differential centrifugation kana density gradient centrifugation, dzinopa mazinga akasiyana e resolution. Kunyange hazvo density gradient centrifugation ichipa kupatsanurwa kwepamusoro-soro, inoda mamwe matanho uye michina yakasarudzika. Differential centrifugation, kunyangwe iri nyore, inogona kunge isina resolution yakakwana, zvichikonzera ma bands anopindirana muzvikamu zvinobva. Vaongorori vanofanirwa kunyatsofunga nezve resolution inodiwa yekushandisa kwavo chaiyo uye kusarudza nzira yakakodzera ye centrifugation zvinoenderana.

5. Nguva Nemutengo:

Kugadzira centrifugation kunogona kutora nguva uye kunodhura. Kuwana kupatsanurwa kwakakwana uye kupatsanurwa kwezvikamu kunogona kuda matanho akawanda, nguva refu yekuisa centrifugation, uye matekiniki akasarudzika. Semuenzaniso, kuti vawane zvikamu zvakacheneswa zvakanyanya, vaongorori vangangoda kuita denderedzwa rakatevedzana rekuisa centrifugation, zvichikonzera kuwedzerwa kwenguva uye mari yekushandisa. Pamusoro pezvo, marotor ne centrifuge akasarudzika anokwanisa kupatsanurwa nekukurumidza uye kwepamusoro-soro anogona kudhura kuwana nekuchengetedza, zvichiita kuti zvive zvakakosha kuyera mabhenefiti nemitengo inobatanidzwa.

Mhedziso:

Kugadzira centrifugation kwakachinja nzvimbo yekupatsanura nekuparadzanisa, zvichigonesa vaongorori kuwana zvikamu zvakacheneswa kubva mumisanganiswa yakaoma. Zvisinei, inewo miganho yayo. Mabhandi anopindirana, kusanganiswa kwemvura, kusvibiswa kwesampuro, kugadziriswa kushoma, uye nguva nemitengo zvinoenderana nazvo zvese zvinopa matambudziko pakushandiswa kwe centrifugation zvakanaka. Pasinei nemiganho iyi, centrifugation ichiri nzira inokosha mune dzakawanda dzidzo dzesainzi, uye kufambira mberi kuri kuenderera mberi kunovavarira kukunda matambudziko aya nekuvandudza kushanda kwayo uye kushanda kwayo. Vaongorori vanofanirwa kunyatsoongorora miganho uye kugadzirisa maitiro avo e centrifugation kuti vave nechokwadi chekuti kupatsanurana nekupatsanurana zvinobudirira kune mashandisirwo avo chaiwo.