dab tsi yog cov kev txwv ntawm kev faib ua feem thiab kev sib cais los ntawm centrifugation
Cov Kev Txwv ntawm Fractionation thiab Kev Sib Cais los ntawm Centrifugation yog dab tsi?
Kev Taw Qhia:
Kev siv centrifugation yog ib txoj kev siv dav hauv chav kuaj mob rau kev sib cais thiab faib cov khoom sib txawv ntawm cov khoom sib xyaw raws li lawv qhov ceev siv lub hauv paus ntsiab lus ntawm kev tso dej. Nws cuam tshuam nrog kev tig ceev ntawm cov qauv ntawm qhov ceev ceev, tso cai rau cov khoom me me lossis cov khoom sib xyaw kom nyob hauv qab thaum cov khoom me me tseem nyob rau sab saum toj. Txawm hais tias centrifugation yog ib qho cuab yeej muaj zog, nws muaj nws cov kev txwv. Hauv tsab xov xwm no, peb yuav tshawb nrhiav cov kev txwv ntawm kev faib thiab kev sib cais los ntawm centrifugation, qhia txog qee qhov teeb meem tseem ceeb uas cov kws tshawb fawb ntsib thaum siv cov txheej txheem no.
1. Qhov Loj thiab Qhov Ceev ntawm Cov Khoom Me Me:
Ib qho ntawm cov yam tseem ceeb tshaj plaws uas txiav txim siab qhov ua tiav ntawm kev faib ua feem thiab kev sib cais los ntawm centrifugation yog qhov loj me thiab qhov ceev ntawm cov khoom me me. Centrifugation muaj txiaj ntsig zoo rau kev sib cais cov khoom me me uas muaj qhov sib txawv loj hauv qhov ceev, xws li organelles, macromolecules, lossis cov khib nyiab ntawm tes. Txawm li cas los xij, thaum cov khoom me me lossis cov khoom muaj qhov ceev lossis qhov ntev zoo sib xws, kev sib cais kom raug yuav nyuaj. Lub zog centrifugal yuav tsis txaus kom ua tiav kev sib cais tag nrho, ua rau muaj cov kab sib tshooj lossis kev faib ua feem tsis tiav.
2. Kev sib xyaw ua ke ntawm cov av noo:
Kev sib koom ua ke yog hais txog qhov teeb meem ntawm qee cov khoom lossis cov khoom me me uas sib xyaw ua ke thaum lub sijhawm centrifugation, ua rau nws nyuaj rau cais lawv tag nrho. Qhov xwm txheej no tshwm sim thaum qhov sib txawv ntawm qhov ceev ntawm cov khoom me me, ua rau muaj kev sib koom ua ke thaum lub sijhawm centrifugation. Kev sib koom ua ke tuaj yeem ua teeb meem tshwj xeeb thaum ua haujlwm nrog cov khoom sib xyaw ua ke, xws li cov qauv tshuaj lom neeg lossis cov khoom sib xyaw ua ke, qhov twg kev sib cais ntawm cov khoom tshwj xeeb yog qhov nyuaj vim lawv cov yam ntxwv zoo sib xws.
3. Kev Ua Phem Rau Cov Qauv:
Thaum lub sijhawm centrifugation, kev ua qias tuaj yeem yog qhov nyuaj heev. Nws yog ib qho tseem ceeb kom ntseeg tau tias cov xwm txheej thiab cov kev teeb tsa centrifugation tau ua kom zoo tshaj plaws kom txo qis kev ua qias tuaj ntawm lub rotor, tubing, lossis cov raj sau. Cov pa phem tuaj yeem cuam tshuam rau cov txheej txheem fractionation, ua rau cov qauv tsis huv. Yuav tsum tau ua tib zoo saib xyuas kev xaiv cov ntaub ntawv rotor tsim nyog, siv cov raj centrifugation tsim nyog, thiab siv cov txheej txheem ntxuav kom zoo kom tiv thaiv kev ua qias tuaj ntawm cov qauv.
4. Kev daws teeb meem tsawg:
Qhov kev daws teeb meem ntawm kev faib ua feem thiab kev sib cais los ntawm centrifugation feem ntau nyob ntawm kev xaiv ntawm centrifuge thiab rotor. Cov txheej txheem centrifugation sib txawv, xws li kev sib txawv centrifugation lossis kev sib txawv ntawm qhov ceev, muab ntau qib kev daws teeb meem. Thaum kev sib txawv ntawm qhov ceev muab kev sib cais siab, nws xav tau cov kauj ruam ntxiv thiab cov khoom siv tshwj xeeb. Kev sib txawv centrifugation, thaum yooj yim dua, tej zaum yuav muaj kev daws teeb meem tsawg, ua rau muaj cov kab sib tshooj hauv cov feem tshwm sim. Cov kws tshawb fawb yuav tsum xav txog qhov kev daws teeb meem xav tau rau lawv daim ntawv thov tshwj xeeb thiab xaiv txoj kev centrifugation tsim nyog raws li.
5. Lub Sijhawm thiab Tus Nqi:
Kev centrifugation tuaj yeem yog ib qho txheej txheem siv sijhawm thiab kim heev. Kev ua tiav qhov kev sib cais thiab kev faib ua feem zoo tshaj plaws yuav xav tau ntau kauj ruam, lub sijhawm centrifugation ntev dua, thiab cov txheej txheem tshwj xeeb. Piv txwv li, kom tau txais cov feem pua uas huv heev, cov kws tshawb fawb yuav tsum ua cov kev sib hloov ntawm centrifugation, ua rau muaj kev nqis peev ntau dua thiab cov peev txheej. Tsis tas li ntawd, cov rotors tshwj xeeb thiab centrifuges uas muaj peev xwm ua qhov kev sib cais ceev thiab kev daws teeb meem siab tuaj yeem kim heev rau kev tau txais thiab tswj hwm, ua rau nws tseem ceeb kom ntsuas qhov txiaj ntsig piv rau cov nqi cuam tshuam.
Xaus lus:
Kev siv centrifugation tau hloov pauv txoj kev ua haujlwm ntawm kev faib ua feem thiab kev sib cais, ua rau cov kws tshawb fawb tau txais cov khoom huv los ntawm cov khoom sib xyaw ua ke. Txawm li cas los xij, nws kuj muaj nws cov kev txwv. Cov kab sib tshooj, kev sib koom ua ke, kev ua qias tuaj ntawm cov qauv, kev daws teeb meem tsawg, thiab cov yam ntxwv ntawm lub sijhawm thiab tus nqi cuam tshuam txhua yam ua rau muaj kev cov nyom rau kev siv centrifugation kom zoo. Txawm hais tias muaj cov kev txwv no, centrifugation tseem yog ib txoj hauv kev muaj txiaj ntsig hauv ntau yam kev tshawb fawb, thiab kev nce qib tas mus li tsom mus rau kev kov yeej cov kev cov nyom no thiab txhim kho nws txoj kev ua haujlwm zoo thiab kev ua haujlwm zoo. Cov kws tshawb fawb yuav tsum ua tib zoo soj ntsuam cov kev txwv thiab ua kom zoo dua lawv cov txheej txheem centrifugation kom ntseeg tau tias kev sib cais thiab kev faib ua feem ua tiav rau lawv cov ntawv thov tshwj xeeb.
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