kāore e taea te rewa. ka wehea ngā waiwai mā te centrifuge
1. He Kupu Whakataki ki ngā Wairewa Kore-Wai me te Whakapūtanga
2. Te Pūtaiao kei Muri i te Whakapūngao
3. Te Whai Huatanga Wehewehe o ngā Tauira Pūwero Rerekē
4. Te Arotau i ngā Tawhā Whakapūtanga mō te Wehenga Wairewa Whai Hua
5. Ngā Whakamahinga o te Centrifugation i roto i ngā Ahumahi Rerekē
6. Whakatau: Te Whakamahi i te Centrifugation mō te Wehenga Wairewa Kore-Waihanga Whai Hua
1. He Kupu Whakataki ki ngā Wairewa Kore-Wai me te Whakapūtanga
Ko ngā matūriki kore-rewa he matūriki totoka ka puta ina tauhohe ngā matū e rua, neke atu rānei, ki roto i tētahi otinga, ā, ka puta he matū totoka. Ka puta ake ēnei matūriki i roto i ngā tukanga pūtaiao me ngā tukanga ahumahi, tae atu ki te hanga matū, te whanaketanga rongoā, me te tātari taiao. Ko tētahi o ngā tikanga tino noa e whakamahia ana mō te wehewehe i ngā matūriki kore-rewa mai i te otinga ko te centrifugation.
Ko te centrifugation he tikanga e whakamahi ana i ngā mātāpono o te whakatō me te kaha ā-papa hei wehe i ngā matūriki totoka mai i te ranunga wai. Kei roto i tēnei ko te whakamahinga o tētahi taputapu hurihuri tere-rahi, e mōhiotia ana ko te centrifuge, e whakamahi ana i te kaha centrifuge ki te tauira. Mā tēnei kaha ka heke ngā matūriki matotoru, pērā i ngā matūriki kore-rewa, ki raro i te ngongo centrifuge, ka taea ai te wehe i a rātou mai i te wai i runga.
2. Te Pūtaiao kei Muri i te Whakapūngao
Ko te whakapūtanga e whakawhirinaki ana ki ngā rerekētanga o ngā mātotoru me ngā rahi o ngā matūriki i roto i te ranunga. Ina whakaurua he tauira ki roto i te ngongo whakapūtanga, ā, ka tukuna ki te tere hurihuri teitei, ka mahi te kaha whakapūtanga hei "kaha ā-papatipu" ka whakateretere i ngā matūriki ki raro i te ngongo. He rite tonu tēnei kaha ki te tere o te hurihuri, ā, he rite whakamuri ki te radius mai i te pokapū o te hurihuri.
I te hurihuri o te tauira, ka noho tonu ngā matūriki iti ake, iti iho te matotoru, e iri ana i roto i te wai, ā, ka ngāwari te ringihia. He rerekē, ko ngā matūriki nui ake, nui ake te matotoru, pērā i ngā matūriki kore e rewa, ka tere te kohikohi ki raro o te ngongo nā te mea kāore e taea te noho iri tonu i te kaha o te porotītaha. Ka āwhinahia tēnei wehenga e te hanganga o te pire, he puranga matūriki totoka kua whakakīia ki raro o te ngongo porotītaha.
3. Te Whai Huatanga Wehewehe o ngā Tauira Pūwero Rerekē
Ko te whai huatanga o te wehenga o ngā matū kore-rewa mā te whakamahi i te centrifugation e whakawhirinaki ana ki te tauira centrifuge e whakamahia ana. Ko ngā hoahoa centrifuge rerekē, pērā i ngā centrifuge tere-rahi, ngā centrifuge ultra-ultrasonic, me ngā centrifuge pouaka whakamātao, e tohuhia ana e ngā rerekētanga o te tere hurihuri mōrahi, te whakahaere pāmahana, me te momo rotor e whakamahia ana. He mea nui ēnei āhuatanga ki te whakatutuki i ngā hua wehenga tino pai.
Ka taea e ngā pūwero tere-rahi te eke ki te tere o te 20,000 hurihanga ia meneti (rpm), engari ka taea e ngā pūwero tino-rahi te whakaputa tere neke atu i te 100,000 rpm. Ko te teitei ake o te tere hurihuri, ko te nui ake o te kaha pūwero e whakamahia ana, ka hua ake he pai ake te wehenga. Engari, ko ngā pūwero pouaka whakamātao ka whakarato i te whakahaere pāmahana i te wā o te tukanga hei ārai i te whakahekenga wera o ngā tauira tairongo.
4. Te Arotau i ngā Tawhā Whakapūtanga mō te Wehenga Wairewa Whai Hua
Hei whakatutuki i te wehenga whai hua o ngā matū kore-rewa, me whai whakaaro me te arotau i ētahi tawhā. He mea nui te whiriwhiri i te tere me te roa o te wā e tika ana mō te centrifuge, nā te mea ko te nui rawa atu i te tere matua mō tētahi matū motuhake ka taea te whakatārewa anō i ngā matūriki e tino hiahiatia ana. Hei tāpiri, ko te roa o ngā wā centrifuge ka arahi pea ki te hanganga o ngā matūriki tuarua, te kohikohinga matūriki kāore e hiahiatia rānei.
He mea nui anō hoki te whiriwhiri i tētahi rotor e tika ana kia pai ai te wehenga. Ko ngā rotor koki-pūmau te mea pai mō te tere o te whakatōnga matūriki i roto i ngā rahinga iti, ko ngā rotor peere-hurihuri te mea pai mō ngā rahinga tauira nui me ngā pikinga. He mea nui anō hoki te taurite tika o te rotor centrifuge hei ārai i te kino pea ki ngā taputapu me te ngaronga o te tauira.
5. Ngā Whakamahinga o te Centrifugation i roto i ngā Ahumahi Rerekē
He mea nui te mahi a te centrifugation i roto i ngā mahi pūtaiao me ngā mahi ahumahi maha. I roto i te umanga rongoā, ka whakamahia ngā centrifugation i roto i ngā tukanga hanga me te whakawhanaketanga rongoā, e āhei ai te wehewehe i ngā kai rongoā hohe mai i ngā poke, i ngā hua taha rānei. I roto i te mara o te tātari taiao, ka āwhina te centrifugation i te wehenga o ngā matūriki mai i ngā tauira wai para, e āwhina ana i ngā tukanga purenga wai.
Hei tāpiri atu ki tēnā, he whānuitia te whakamahinga o te centrifugation i roto i ngā taiwhanga rangahau koiora hei wehe i ngā wāhanga pūtau, pērā i ngā pūmua, te DNA, me ngā whekau pūtau. He mea nui tēnei tikanga wehewehe mō te ako i ngā hanganga pūtau me te mārama ki ngā mahi pūtau. Ka kitea hoki ngā whakamahinga o te centrifugation i roto i ngā ahumahi kai me te inu, ka āwhina i te wehenga o ngā mea totoka kāore e hiahiatia mai i ngā hua wai, kia pai ai te kounga o ngā hua whakamutunga.
6. Whakatau: Te Whakamahi i te Centrifugation mō te Wehenga Wairewa Kore-Waihanga Whai Hua
He tikanga kaha, he tikanga whānui hoki te whakamahi i te centrifugation mō te wehewehe i ngā matū kore e rewa i roto i ngā ranunga wai. He mea nui te mārama ki ngā mātāpono o te centrifugation me ngā āhuatanga e pā ana ki te whai huatanga o te wehenga kia eke ki ngā hua tino pai. Mā te whiriwhiri tika me te arotau i ngā tawhā centrifuge, ka taea e ngā kaipūtaiao me ngā miihini te wehewehe me te whiwhi i ngā matū whai hua, e āhei ai te tātari me te whakamahi anō i roto i ngā momo ahumahi.
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