tuaj yeem tsis yaj. cov dej nag yuav tsum tau sib cais los ntawm centrifuge
1. Kev Taw Qhia Txog Cov Khoom Uas Tsis Yaj Tau Thiab Centrifugation
2. Kev Tshawb Fawb Tom Qab Centrifugation
3. Kev Sib Cais Zoo ntawm Cov Qauv Centrifuge Sib Txawv
4. Kev Txhim Kho Cov Kev Ntsuas Centrifugation rau Kev Sib Cais Precipitate Zoo
5. Cov ntawv thov ntawm Centrifugation hauv ntau yam lag luam
6. Xaus Lus: Siv Centrifugation rau Kev Sib Cais Cov Khoom Uas Tsis Yaj Tau Zoo
1. Kev Taw Qhia Txog Cov Khoom Uas Tsis Yaj Tau Thiab Centrifugation
Cov khoom tawg uas tsis yaj yog cov khoom khov uas tsim los thaum ob lossis ntau yam tshuaj sib xyaw ua ke hauv cov kua, ua rau muaj cov khoom khov. Cov khoom tawg no tuaj yeem tshwm sim hauv ntau yam txheej txheem kev tshawb fawb thiab kev lag luam, suav nrog kev tsim tshuaj lom neeg, kev tsim tshuaj, thiab kev tshuaj xyuas ib puag ncig. Ib qho ntawm cov txheej txheem feem ntau siv los cais cov khoom tawg uas tsis yaj ntawm cov kua yog centrifugation.
Kev centrifugation yog ib txoj kev siv cov ntsiab cai ntawm kev tso dej thiab lub zog gravitational los cais cov khoom khov tawm ntawm cov kua sib tov. Nws cuam tshuam nrog kev siv lub cuab yeej tig ceev ceev, hu ua centrifuge, uas siv lub zog centrifugal rau cov qauv. Lub zog no ua rau cov khoom ntom dua, zoo li cov precipitates tsis yaj, kom txav mus rau hauv qab ntawm lub raj centrifuge, ua rau lawv sib cais los ntawm cov kua supernatant.
2. Kev Tshawb Fawb Tom Qab Centrifugation
Kev centrifugation yog nyob ntawm qhov sib txawv ntawm qhov ceev thiab qhov loj ntawm cov khoom me me hauv ib qho sib tov. Thaum ib qho qauv raug muab tso rau hauv lub raj centrifuge thiab raug rau qhov ceev ceev, lub zog centrifugal ua haujlwm ua "lub ntiajteb txawj nqus dag" thiab ua kom cov khoom me me mus rau hauv qab ntawm lub raj. Lub zog no yog proportional ncaj qha rau qhov ceev ntawm kev tig thiab inversely proportional rau lub vojvoog ntawm qhov chaw ntawm kev tig.
Thaum cov qauv tig, cov khoom me me thiab tsis ntom ntom tseem nyob hauv cov kua thiab yooj yim decanting. Qhov sib txawv, cov khoom loj dua thiab ntom ntom, xws li cov dej tsis yaj, sai sai sib sau ua ke ntawm qab ntawm lub raj vim lawv tsis muaj peev xwm nyob twj ywm dai tawm tsam lub zog centrifugal siv. Qhov kev sib cais no yog ua kom yooj yim los ntawm kev tsim cov pellet, ib pawg khoom khov kho ntawm qab ntawm lub raj centrifuge.
3. Kev Sib Cais Zoo ntawm Cov Qauv Centrifuge Sib Txawv
Qhov ua tau zoo ntawm kev sib cais ntawm cov precipitates uas tsis yaj siv centrifugation feem ntau nyob ntawm tus qauv centrifuge siv. Cov qauv centrifuge sib txawv, xws li kev kub ceev, ultracentrifuges, thiab cov centrifuges txias, yog tus cwj pwm los ntawm kev hloov pauv ntawm qhov ceev tshaj plaws, kev tswj kub, thiab hom rotor siv. Cov yam no ua lub luag haujlwm tseem ceeb hauv kev ua tiav cov txiaj ntsig sib cais zoo tshaj plaws.
Cov centrifuges ceev ceev tuaj yeem ncav cuag qhov ceev txog li 20,000 revolutions ib feeb (rpm), thaum ultracentrifuges muaj peev xwm tsim cov ceev tshaj 100,000 rpm. Qhov ceev tig siab dua, lub zog centrifugal siv ntau dua, ua rau kev sib cais zoo dua. Cov centrifuges txias, ntawm qhov tod tes, muab kev tswj kub thaum lub sijhawm ua haujlwm kom tiv thaiv kev puas tsuaj thermal ntawm cov qauv rhiab heev.
4. Kev Txhim Kho Cov Kev Ntsuas Centrifugation rau Kev Sib Cais Precipitate Zoo
Yuav kom ua tiav kev sib cais cov dej nag uas tsis yaj tau zoo, yuav tsum xav txog ntau yam kev ntsuas thiab ua kom zoo. Kev xaiv qhov ceev thiab lub sijhawm centrifuge tsim nyog yog qhov tseem ceeb, vim tias dhau qhov ceev tseem ceeb rau ib qho dej nag tshwj xeeb tuaj yeem ua rau cov khoom me me uas xav tau heev rov qab los ua dua. Tsis tas li ntawd, lub sijhawm centrifugation ntev dua tuaj yeem ua rau muaj cov dej nag thib ob lossis cov khoom me me uas tsis xav tau.
Kev xaiv lub rotor uas tsim nyog kuj tseem ceeb heev rau kev sib cais zoo tshaj plaws. Cov rotors uas muaj lub kaum sab xis yog qhov zoo tshaj plaws rau kev tso cov khoom me me sai sai, thaum cov rotors swinging-bucket yog qhov zoo tshaj plaws rau cov qauv loj dua thiab cov gradients. Kev sib npaug ntawm lub centrifuge rotor kuj tseem ceeb heev kom tiv thaiv kev puas tsuaj rau cov khoom siv thiab kev poob qauv.
5. Cov ntawv thov ntawm Centrifugation hauv ntau yam lag luam
Kev siv centrifugation ua lub luag haujlwm tseem ceeb hauv ntau yam kev siv hauv kev tshawb fawb thiab kev lag luam. Hauv kev lag luam tshuaj, centrifuges raug siv thaum lub sijhawm tsim tshuaj thiab cov txheej txheem tsim khoom, ua rau muaj kev sib cais cov khoom xyaw nquag ntawm cov tshuaj los ntawm cov khoom tsis huv lossis cov khoom seem. Hauv kev tshuaj xyuas ib puag ncig, centrifugation pab sib cais cov khoom me me ntawm cov qauv dej khib nyiab, pab hauv cov txheej txheem ntxuav dej.
Ntxiv mus, centrifugation siv dav hauv cov chaw soj nstuam tshawb fawb txog tsiaj txhu los cais cov khoom hauv lub cev, xws li cov protein, DNA, thiab cov organelles hauv lub cev. Cov txheej txheem sib cais no yog qhov tseem ceeb rau kev kawm txog cov qauv ntawm lub cev thiab nkag siab txog kev ua haujlwm ntawm lub cev. Centrifugation kuj pom cov ntawv thov hauv kev lag luam khoom noj thiab dej haus, qhov twg nws pab txhawb kev sib cais cov khoom khov tsis xav tau los ntawm cov khoom ua kua, kom ntseeg tau tias cov khoom kawg zoo.
6. Xaus Lus: Siv Centrifugation rau Kev Sib Cais Cov Khoom Uas Tsis Yaj Tau Zoo
Kev siv centrifugation muaj ib txoj kev siv zog thiab dav dav rau kev sib cais cov dej nag uas tsis yaj los ntawm cov kua sib xyaw. Kev nkag siab txog cov ntsiab cai tom qab centrifugation thiab cov yam ntxwv uas cuam tshuam rau kev sib cais zoo yog qhov tseem ceeb rau kev ua tiav cov txiaj ntsig zoo tshaj plaws. Nrog kev xaiv thiab kev ua kom zoo ntawm cov txheej txheem centrifuge, cov kws tshawb fawb thiab cov engineers tuaj yeem cais thiab tau txais cov dej nag muaj nqis, uas tso cai rau kev tshuaj xyuas ntxiv thiab kev siv hauv ntau yam lag luam.
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