thaum cov ntshav tshiab raug centrifuged nws cais tawm
Thaum Cov Ntshav Tshiab raug Centrifuged, nws sib cais
Kev Taw Qhia:
Cov txheej txheem ntawm centrifugation hauv cov chaw kuaj mob ua lub luag haujlwm tseem ceeb hauv kev sib cais ntau yam khoom ntawm cov ntshav tshiab. Cov txheej txheem no siv lub hauv paus ntsiab lus ntawm qhov sib txawv ntawm qhov ceev los cais cov qe ntshav liab, plasma, thiab platelets. Los ntawm kev tig cov qauv ntshav hauv lub centrifuge, cov khoom sib cais ua cov txheej sib txawv, uas tso cai rau kev tshuaj xyuas ntxiv thiab kev kuaj mob. Hauv tsab xov xwm no, peb yuav tshawb nrhiav cov txheej txheem ntawm centrifugation thiab nws qhov tseem ceeb hauv kev siv kho mob sib txawv.
Kev Nkag Siab Txog Centrifugation:
1. Lub Ntsiab Cai ntawm Centrifugation:
Kev centrifugation yog siv lub hauv paus ntsiab lus ntawm kev tso dej tawm, uas hais tias cov khoom me me dua yuav nyob hauv qab thaum muab tso rau hauv cov kua dej. Hauv cov ntshav, qhov no txhais tau tias cov qe ntshav liab, uas tuab dua li cov plasma, yuav nyob hauv qab thiab tsim cov txheej hauv qab tshaj plaws tom qab centrifugation.
2. Hom Centrifuges:
Muaj ntau hom centrifuges siv hauv chav kuaj mob. Cov no suav nrog cov centrifuge ceev ceev, ultracentrifuge, thiab microcentrifuge. Cov centrifuges ceev ceev muaj peev xwm loj thiab siv rau kev sib cais ntau. Ultracentrifuges, ntawm qhov tod tes, tuaj yeem ua tiav qhov ceev ceev dua thiab siv rau kev sib cais cov khoom me me, xws li cov kab mob thiab macromolecules. Microcentrifuges yog compact thiab feem ntau yog siv rau kev kuaj mob hauv chav kuaj mob.
Cov txheej txheem ntawm Centrifugation:
1. Kev Sau Qauv:
Ua ntej siv lub tshuab centrifuge, yuav muab cov ntshav coj los sau ua ke siv lub koob txhaj tshuaj lossis koob txhaj tshuaj. Cov tshuaj Anticoagulants raug ntxiv rau kom tiv thaiv kev txhaws thaum lub sijhawm ua haujlwm. Tom qab ntawd yuav muab cov ntshav coj mus rau hauv lub raj centrifuge kom zoo.
2. Kev Tso Rau Hauv Lub Centrifuge:
Cov raj centrifuge uas muaj cov qauv ntshav raug muab tso rau hauv lub rotor centrifuge. Nws yog ib qho tseem ceeb kom faib cov raj kom sib npaug zos rau kev tig ruaj khov thiab kev sib cais kom raug.
3. Teem Qhov Ceev thiab Lub Sijhawm:
Qhov ceev thiab lub sijhawm teeb tsa tsim nyog sib txawv raws li qhov kev sib cais xav tau thiab hom centrifuge siv. Feem ntau, qhov ceev siab dua yog siv los cais cov qe ntshav liab ntawm cov ntshav.
4. Kev Centrifuge:
Thaum twg qhov ceev thiab lub sijhawm teeb tsa tau teeb tsa lawm, lub centrifuge yuav pib ua haujlwm. Thaum lub rotor tig sai sai, cov ntshav sib cais vim lawv qhov sib txawv ntawm qhov ceev. Cov qe ntshav liab txav mus rau hauv qab (pellet), thaum cov plasma nce mus rau saum toj, tawm hauv ib txheej ntshiab nyob nruab nrab hu ua buffy coat.
Cov ntawv thov ntawm Centrifugation hauv tshuaj:
1. Kev Hloov Ntshav:
Kev siv lub tshuab centrifugation yog qhov tseem ceeb rau kev sau thiab sib cais cov khoom xyaw ntawm cov ntshav thaum lub sijhawm hloov ntshav. Los ntawm kev sib cais cov qe ntshav liab, cov ntshav plasma, thiab cov platelets, nws yuav yooj yim dua los rho tawm cov khoom tshwj xeeb rau kev hloov ntshav thiab ua kom tus neeg mob muaj kev nyab xeeb.
2. Kev Kuaj Mob:
Hauv kev kuaj mob, kev siv centrifugation dav dav los ua ntau yam kev kuaj. Piv txwv li, kev suav ntshav tag nrho (CBC) xav tau kev sib cais cov qe ntshav liab ntawm cov ntshav hauv cov ntshav kom ntsuas cov hematocrit thiab hemoglobin. Tsis tas li ntawd, lwm yam kev kuaj, xws li kev kuaj mob siab thiab lub raum, vam khom kev siv centrifugation los cais cov serum lossis plasma rau kev tshuaj xyuas.
3. Kev Tshawb Fawb Txog Cellular:
Kev siv centrifugation pab cais thiab ntxuav cov cell tshwj xeeb rau kev tshawb fawb. Los ntawm kev cais cov cell ntawm lwm cov ntshav, cov kws tshawb fawb tuaj yeem kawm txog cov pej xeem tshwj xeeb, xws li cov cell ntshav dawb lossis cov cell noob, kom ntxaws.
4. Kev Tsim Tshuaj:
Hauv kev lag luam tshuaj, centrifugation feem ntau siv thaum lub sijhawm tsim tshuaj thiab cov txheej txheem tsim khoom. Thaum lub sijhawm ua tiav, centrifugation yog siv los cais cov cell ntawm cov kua zaub fermentation, sau cov khoom cell, thiab tshem tawm cov khib nyiab cell.
Xaus lus:
Kev siv lub tshuab centrifugation yog ib txoj kev tseem ceeb uas siv hauv cov chaw kuaj mob los cais cov ntshav sib xyaw rau ntau yam kev siv. Los ntawm kev siv lub hauv paus ntsiab lus ntawm kev sib txawv ntawm cov av noo thiab qhov ceev, kev siv lub tshuab centrifugation tso cai rau kev cais cov khoom tshwj xeeb, pab txhawb kev kuaj mob, kev tshawb fawb, thiab kev tsim tshuaj. Nrog rau kev nce qib ntawm thev naus laus zis, cov txheej txheem centrifugation txuas ntxiv mus, ua rau kev sib cais ntshav ua haujlwm tau zoo dua thiab raug dua.
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