Yuav ua li cas cais cov ntshav hauv lub centrifuge
Nkag Siab Txog Lub Centrifuge Thiab Nws Lub Luag Haujlwm Hauv Kev Sib Cais Ntshav
Kev siv centrifugation yog ib qho txheej txheem tseem ceeb uas siv rau ntau qhov chaw tshawb fawb thiab kev lag luam, suav nrog kev kho mob thiab chaw kuaj mob. Los ntawm lub zog centrifugal uas tsim los ntawm lub rotor tig, cov khoom hauv ib qho qauv tuaj yeem sib cais tau zoo thiab ua tau zoo raws li lawv qhov ceev. Kev sib cais ntshav yog ib qho ntawm cov kev siv centrifugation feem ntau, ua haujlwm tseem ceeb hauv cov txheej txheem kuaj mob, kev khaws ntshav, thiab kev tshawb fawb. Hauv tsab xov xwm no, peb yuav nkag mus rau hauv lub ntiaj teb zoo kawg nkaus ntawm centrifugation thiab tshawb pom tias cov ntshav raug sib cais li cas siv cov khoom siv no.
Cov Cheebtsam ntawm Centrifuge
Lub centrifuge feem ntau muaj peb yam tseem ceeb: lub cev muaj zog, rotor, thiab cov tuav raj. Lub cev muaj zog tsim lub zog tig uas xav tau rau centrifugation, feem ntau siv hluav taws xob. Lub rotor, tseem hu ua lub tais lossis lub taub hau, yog lub nruas tig uas muab cov qauv tso rau. Nws muaj ntau yam qauv, suav nrog lub kaum sab xis thiab lub rotors tig tawm, txhua tus haum rau cov hom kev sib cais tshwj xeeb. Cov tuav raj tuav cov raj kuaj kom ruaj khov thaum lub sijhawm centrifugation, tiv thaiv kev xau thiab xyuas kom meej tias muaj kev sib hloov zoo.
Kev Sib Tw Centrifugation Sib Txawv: Kauj Ruam Sib Cais Pib
Yuav kom nkag siab txog kev sib cais ntshav tshwm sim li cas hauv lub centrifuge, peb yuav tsum tshawb nrhiav lub hauv paus ntsiab lus ntawm kev sib txawv centrifugation. Cov txheej txheem no suav nrog kev muab cov qauv rau ntau kauj ruam centrifugation, maj mam nce qhov ceev kom cais cov khoom sib xyaw raws li lawv qhov ceev. Hauv cov ntsiab lus ntawm cov ntshav, cov txheej txheem sib cais lub hom phiaj yog cais cov qe ntshav liab (RBCs), cov qe ntshav dawb (WBCs), platelets, thiab plasma rau kev tshuaj xyuas ntxiv.
Cov Yam Cuam Tshuam Rau Kev Sib Cais Ntshav
Muaj ntau yam uas cuam tshuam rau qhov ua tau zoo ntawm kev sib cais ntshav hauv lub tshuab centrifuge. Qhov ceev lossis lub zog ntawm kev centrifugation, ntsuas hauv revolutions ib feeb (RPM) lossis lub zog centrifugal (RCF), yog ib qho tseem ceeb. Qhov ceev dua ua rau kev sib cais ntau dua, tab sis lub zog centrifugal ntau dhau yuav ua rau qee cov ntshav puas tsuaj. Lub sijhawm ntawm kev centrifugation thiab qhov kub uas nws tshwm sim kuj cuam tshuam rau kev sib cais ntshav. Cov xwm txheej zoo tshaj plaws rau kev sib cais ntshav nyob ntawm lub hom phiaj tshwj xeeb ntawm kev sim lossis txheej txheem, nrog rau hom ntshav qauv uas tau ua.
Cov Txheej Txheem ntawm Kev Sib Cais Ntshav
Tam sim no, cia peb kawm txog cov kauj ruam uas siv lub tshuab centrifuge los cais cov ntshav. Ua ntej, cov qauv ntshav, feem ntau yog sau rau hauv lub raj uas muaj tshuaj anticoagulant, raug muab tso rau hauv cov tuav raj uas tsim nyog hauv lub rotor. Tom qab ntawd lub rotor raug xauv rau hauv qhov chaw, kom ntseeg tau tias cov qauv ntshav raug tuav ruaj ntseg kom tsis txhob muaj teeb meem thaum lub sijhawm centrifuge.
Tom qab ntawd, lub centrifuge yuav pib ua haujlwm, thiab lub rotor yuav nrawm mus rau qhov ceev uas xav tau. Thaum lub rotor tig, cov khoom sib xyaw ntawm cov ntshav uas tuab tshaj plaws, xws li cov qe ntshav liab, raug rub mus rau ntug sab nraud ntawm lub raj. Lub sijhawm ntawd, cov plasma, uas yog cov kua ntawm cov ntshav, yuav sib sau ua ke ze ntawm nruab nrab ntawm lub raj. Qhov kev sib cais no tshwm sim vim yog qhov sib txawv ntawm cov khoom sib xyaw ntawm cov ntshav.
Tom qab lub sijhawm teem tseg lossis thaum tau txais kev sib cais raws li qhov xav tau, lub centrifuge maj mam qeeb zuj zus, ua rau nws nres. Yuav tsum ceev faj kom tsis txhob cuam tshuam cov txheej uas tau sib cais thaum tuav cov raj. Thaum kawg, lub raj raug tshem tawm ntawm lub rotor, thiab cov txheej uas tau sib cais lawm yuav npaj txhij rau kev tshuaj xyuas lossis khaws cia ntxiv.
Xaus lus, centrifugation ua lub luag haujlwm tseem ceeb hauv kev sib cais cov ntshav, ua rau muaj kev sib cais cov khoom ntshav tshwj xeeb rau kev kuaj mob, kev tshawb fawb, thiab kev kho mob. Cov txheej txheem no suav nrog kev sib txawv centrifugation, qhov twg qhov sib txawv ntawm cov khoom ntshav ua rau muaj kev sib cais zoo hauv lub rotor. Kev nkag siab txog cov ntsiab cai thiab cov txheej txheem tom qab kev sib cais cov ntshav hauv lub centrifuge ua rau cov kws tshawb fawb, cov kws kho mob, thiab cov kws tshawb fawb siv cov thev naus laus zis no rau ntau yam kev siv.
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