No ke aha i hoʻokaʻawale ʻole ai ka plasma i loko o kahi centrifuge

Hoʻolauna

He ʻenehana hoʻokolohua ka centrifugation i hoʻohana nui ʻia e hoʻokaʻawale i nā mea o nā ʻano like ʻole. Hoʻohana ia i ke kumumanaʻo o ka ikaika centrifugal e hoʻokaʻawale i nā ʻāpana i loko o kahi hui ʻana. Eia nō naʻe, i ke ʻano o ka plasma, ʻaʻole hiki i ka centrifugation ke hana i nā papa ʻokoʻa a i ʻole ka hoʻokaʻawale ʻana. Ke nānā nei kēia ʻatikala i nā kumu ma hope o ka hiki ʻole o ka plasma ke hoʻokaʻawale i loko o kahi centrifuge a hoʻomālamalama i nā waiwai kūikawā o kēia ʻāpana koʻikoʻi o ke koko.

Ke Hoʻomaopopo nei i ka Plasma

ʻO ka plasma, ka ʻāpana wai o ke koko, aia ka wai, nā electrolytes, nā protein, nā hormones, a me nā mea like ʻole. He kuleana koʻikoʻi kona i ka mālama ʻana i ka homeostasis i loko o ke kino ma o ka lawe ʻana i nā meaʻai, nā hormones, a me nā mea ʻōpala. Lawe pū ka plasma i nā antibodies a kōkua i ka hoʻoponopono ʻana i ke koko. ʻO ka hoʻomaopopo ʻana i ka haku mele a me nā waiwai o ka plasma ke kī i ka wehe ʻana i ke kumu ʻaʻole i hoʻokaʻawale ʻia ai ka centrifugation.

ʻO ka mānoanoa a me ka nui o ka plasma

Hōʻike ka plasma i nā waiwai kino kū hoʻokahi e pale ai i ka hoʻokaʻawale ʻana i loko o kahi centrifuge. ʻO kekahi o ia ʻano waiwai ka viscosity. ʻO ka viscosity e pili ana i ke kū'ē ʻana o kahi wai i ke kahe. He haʻahaʻa ka viscosity o ka plasma, e like me ka wai. ʻAʻole e like me nā ʻāpana ʻē aʻe i ke koko, ʻaʻohe kiʻekiʻe o ka plasma e hiki ai ke hoʻokaʻawale pono. ʻO ka hopena, i ka wā e kau ʻia ai nā mana centrifugation, ʻaʻole hiki i ka plasma ke hana i nā papa like ʻole.

Ka Hoʻohālikelike o ka Plasma

He ʻano like ka plasma, ʻo ia hoʻi, e mau ana kona ʻano ma nā wahi a pau. ʻAʻole e like me nā hoʻokuʻu ʻana, nona nā ʻāpana like ʻole i hoʻopuehu ʻia i loko o kahi wai, ʻaʻohe ʻāpana ʻike ʻia o ka plasma. ʻO ka hapa nui, aia nā ion, nā protein, a me nā mea hoʻoheheʻe e hana i kahi hui like. Ke centrifuged ʻia, pale ka homogeneity i ka hoʻokumu ʻia ʻana o kekahi mau papa a i ʻole ka hoʻokaʻawale ʻana.

ʻAʻohe Pellets a i ʻole Sediments

Hilinaʻi ka centrifugation i ka hoʻokaʻawale ʻana o nā ʻāpana ma muli o ko lākou mau mānoanoa. I loko o ke koko, ʻoi aku ka mānoanoa o nā hunaola koko ʻulaʻula a me nā hunaola koko keʻokeʻo ma mua o ka plasma a hiki ke hoʻokaʻawale pono ʻia e ka centrifugation. Hoʻokumu kēia mau hunaola i nā pellets a i ʻole nā ​​sediments ma lalo o ka paipu ma muli o ko lākou mānoanoa kiʻekiʻe. Eia nō naʻe, nele ka plasma i kēlā mau ʻāpana a i ʻole nā ​​sediments, no ka mea, ʻo ia ke kumu nui o ka lawe ʻana i nā mea i hoʻoheheʻe ʻia. Me ka ʻole o kēia mau ʻāpana mānoanoa, ʻaʻole hiki i ke kaʻina hana centrifugation ke hana i kekahi hoʻokaʻawale a i ʻole ka hoʻokumu ʻana o ka papa.

Ke kuleana o nā Anticoagulants

ʻO kekahi kumu e pale ana i ka hoʻokaʻawale ʻana o ka plasma ʻo ia ka hoʻohana ʻana i nā anticoagulants. No ka loaʻa ʻana o ka plasma no nā hoʻokolohua hoʻokolohua like ʻole, hōʻiliʻili pinepine ʻia ke koko i loko o nā ʻōmole e loaʻa ana nā mea anticoagulant. Pale nā ​​​​anticoagulants i ke koko mai ka clotting, ʻoiai lākou e kāohi ana i nā mea coagulation e pono ai no ka hoʻokumu ʻana o ka clot. ʻO ka hopena, noho ke koko i kona ʻano wai, e pale ana i ka hoʻokaʻawale ʻana o ka plasma mai kāna mau ʻāpana cellular.

Hopena

ʻOiai he ʻano hana kūpono ka centrifugation no ka hoʻokaʻawale ʻana i nā ʻāpana me nā densities like ʻole, he pilikia kūikawā ka plasma ma muli o kona mau waiwai a me kona ʻano. ʻO ka viscosity haʻahaʻa o ka plasma, ka homogeneity, ka nele o nā ʻāpana denser, a me ke alo o nā anticoagulants e keakea ana i kona hoʻokaʻawale ʻana me ka hoʻohana ʻana i nā ʻano centrifugation. ʻO ka hoʻomaopopo ʻana i kēia mau ʻano e kōkua i nā mea noiʻi a me nā loea lapaʻau e hoʻohana i nā ʻano hana kūpono e hoʻokaʻawale i ka plasma a aʻo pono i kona mau ʻāpana.