kungani i-plasma ingahlukani ku-centrifuge

Isingeniso

I-Centrifugation iyindlela yelabhorethri esetshenziswa kabanzi ukuhlukanisa izinto ezinobukhulu obuhlukene. Isebenzisa isimiso samandla e-centrifugal ukuhlukanisa izingxenye ngaphakathi kwengxube. Kodwa-ke, endabeni ye-plasma, i-centrifugation yehluleka ukukhiqiza izendlalelo noma ukuhlukana okuhlukile. Lesi sihloko sihlola izizathu zokungakwazi kwe-plasma ukwahlukana ku-centrifuge futhi sikhanyisa izakhiwo ezihlukile zalesi sakhi esibalulekile segazi.

Ukuqonda i-Plasma

I-Plasma, ingxenye ewuketshezi yegazi, inamanzi, ama-electrolyte, amaprotheni, ama-hormone, nezinye izinto ezahlukahlukene. Idlala indima ebalulekile ekugcineni i-homeostasis emzimbeni ngokuthutha izakhamzimba, ama-hormone, nezinto ezilahliwe. I-Plasma iphatha nama-antibodies futhi iyasiza ekulawuleni umfutho wegazi. Ukuqonda ukwakheka kanye nezakhiwo ze-plasma kubalulekile ekutholeni ukuthi kungani i-centrifugation ingabangela ukuhlukana kwayo.

Ukuqina kanye nobuningi be-Plasma

I-Plasma ibonisa izakhiwo zomzimba ezihlukile ezivimbela ukuhlukana ku-centrifuge. Enye yezimfanelo ezinjalo i-viscosity. I-Viscosity ibhekisela ekumelaneni koketshezi nokugeleza. I-Plasma ine-viscosity ephansi kakhulu, efana neyamanzi. Ngokungafani nezinye izingxenye egazini, i-plasma ayinayo i-density ephezulu engayivumela ukuthi ihlukane ngempumelelo. Ngenxa yalokho, uma ingaphansi kwamandla e-centrifuge, i-plasma yehluleka ukwakha izendlalelo ezihlukile.

Ukufana kwe-Plasma

I-plasma iyindlela efanayo, okusho ukuthi ukwakheka kwayo kuhlala kufana kuyo yonke indawo. Ngokungafani nama-suspension, aqukethe izinhlayiya ezihlukene ezihlakazekile oketshezini, i-plasma ayinazo izakhi ezibonakalayo. Iqukethe kakhulu ama-ion, amaprotheni, kanye nezinyibilikisi ezakha ingxube efanayo. Uma i-centrifuged, i-homogeneity ivimbela ukwakheka kwanoma yiziphi izendlalelo noma ukuhlukana.

Ukuntuleka kwama-Pellet noma ama-Sediment

Ukuhlanganisa i-centrifugation kuncike ekuhlukanisweni kwezinhlayiya ngokusekelwe ekubuneni kwazo. Egazini, amangqamuzana abomvu egazi kanye namaseli amhlophe egazi aminyene kune-plasma futhi angahlukaniswa ngempumelelo nge-centrifugation. Lawa maseli akha ama-pellets noma izinsalela phansi kwepayipi ngenxa yobuningi bawo obuphezulu. Kodwa-ke, i-plasma ayinazo izinhlayiya noma izinsalela ezinjalo, njengoba ngokuyinhloko ithwala izinto ezincibilikisiwe. Ngaphandle kwalezi zinhlayiya eziminyene, inqubo yokuhlanganisa i-centrifugation yehluleka ukukhiqiza noma yikuphi ukwakheka kokuhlukana noma ungqimba.

Indima Yama-Anticoagulant

Esinye isici esivimbela ukuhlukaniswa kwe-plasma ukusetshenziswa kwama-anticoagulants. Ukuze kutholakale i-plasma yokuhlolwa okuhlukahlukene kwelebhu, igazi livame ukuqoqwa emapayipini aqukethe ama-anticoagulant agents. Ama-anticoagulants avimbela igazi ekujiyeni, njengoba evimbela izinto ezibangela ukujiya kwegazi ezidingekayo ekwakhekeni kwama-clot. Ngenxa yalokho, igazi lihlala lisesimweni salo soketshezi, livimbela ukuhlukaniswa kwe-plasma ezingxenyeni zalo zamaseli.

Isiphetho

Nakuba i-centrifugation iyindlela ephumelelayo yokuhlukanisa izingxenye ezinobukhulu obuhlukile, i-plasma ibeka inselele eyingqayizivele ngenxa yezakhiwo zayo kanye nokwakheka kwayo. I-viscosity ephansi ye-plasma, ukulingana, ukuntuleka kwezinhlayiya eziqinile, kanye nokuba khona kwama-anticoagulants kuvimbela ukuhlukana kwayo kusetshenziswa izindlela ze-centrifugation. Ukuqonda lezi zici kusiza abacwaningi nochwepheshe bezokwelapha ukusebenzisa amasu afanele ukuhlukanisa i-plasma nokufunda izakhi zayo ngempumelelo.