yiziphi izinto ezingcolisayo ezihlukaniswa yi-centrifuge yegazi

Isihloko

1. Ukuqonda Indima Yokuhlukaniswa Kwezinto Ezingcolisayo

2. Izinhlobo Ezihlukene Zokungcola Kwegazi Okususwe Ngokusebenzisa I-Centrifugation

3. Ukubaluleka Kwesivinini Esifanele Sokucisha Ugesi kanye Nesikhathi Sokuhlukana Okuphumelelayo

4. Amasu Okususa Ukungcola Okuthile Ngokusebenzisa I-Centrifugation

5. Ukusetshenziswa Kwezokwelapha Kwe-Centrifugation Ekususeni Ukungcola Kwegazi

Ukuqonda Indima Yokuhlukaniswa Kwezinto Ezingcolisayo

I-Centrifugation isetshenziswa kabanzi emikhakheni ehlukahlukene, okuhlanganisa nezokwelapha kanye nocwaningo, ukuhlukanisa izingxenye ezahlukene zoketshezi. Emkhakheni wezokwelapha, i-blood centrifugation idlala indima ebalulekile ekuhlukaniseni izinto ezingcolisayo nezinye izinto ezingafuneki kusampula yegazi. Le nqubo ivumela ochwepheshe bezempilo ukuthola izingxenye ezihlanzekile zokuxilonga, ucwaningo, kanye nezinjongo zokwelapha. Kulesi sihloko, sizohlola izici ezahlukene ezingangcolisa igazi nokuthi i-centrifugation isiza kanjani ekuhlukaniseni kwazo ukuqinisekisa imiphumela enembile nethembekile.

Izinhlobo Ezihlukene Zokungcola Kwegazi Okususwe Yi-Centrifugation

Igazi liwuketshezi oluyinkimbinkimbi oluthwala izakhi eziningi ezahlukene, okuhlanganisa amangqamuzana egazi abomvu namhlophe, ama-platelet, i-plasma, kanye nezinto ezahlukahlukene ze-biochemical. Kodwa-ke, lingaqukatha nezinto ezingcolisayo eziphazamisa ukuhlolwa nokuhlaziywa okunembile. I-Centrifugation ingasusa ngempumelelo lezi zinto ezingcolisayo, iqinisekise ubumsulwa bezakhi ezihlukanisiwe.

Esinye sezingcolisi zegazi ezivamile ukungcola kwamangqamuzana. Ngesikhathi sokuqoqwa noma ukuphathwa, amangqamuzana abomvu egazi namaseli amhlophe egazi angakhipha igazi, akhulule okuqukethwe kwawo futhi angase athinte ubuqotho besampula. Ukufaka igazi endaweni eqondile ngesivinini esithile kanye nobude besikhathi kuhlukanisa ukungcola kwamangqamuzana, okuvumela ukuhlolwa okucacile nokunembile kwesampula.

Esinye isingcolisi esikhulu amahlule e-fibrin. Amasampula egazi avame ukuqukatha i-fibrinogen, iphrotheni ebangela ukujiya kwegazi. Uma ishiywe inganakekelwe, i-fibrinogen ingakha amahlule, okwenza kube nzima kochwepheshe bezempilo ukuthola isampula ye-plasma ecacile. Ukususa amahlule kusiza ekususweni kwala mahlule, okuvumela ukuhlukaniswa kwe-plasma engangcolile.

Ukubaluleka Kwesivinini Esifanele Sokucisha Ugesi kanye Nesikhathi Sokuhlukana Okuphumelelayo

Ukuqinisekisa ukuhlukaniswa okuphumelelayo kwezinto ezingcolisayo, isivinini esifanele se-centrifuge kanye nesikhathi kubalulekile. Isivinini se-centrifuge sihambisana namandla adonsela phansi asetshenziswa kusampula, kuyilapho isikhathi sihlobene nobude be-centrifugation.

Ijubane nesikhathi esinganele se-centrifuge kungabangela ukuhlukana okunganele, okuholela ekubeni khona kwezinto ezingcolisayo ezingxenyeni ezihlukanisiwe. Ngokuphambene nalokho, ijubane nesikhathi esiningi kungabangela umonakalo ongafuneki ezingxenyeni zegazi ezifunwayo futhi kungase kuholele emiphumeleni yokuhlolwa enganembile.

Amasu Okususa Ukungcola Okuthile Nge-Centrifugation

Ukuhlanza igazi kudlala indima ebalulekile ekususeni ukungcola okuthile emasampuleni egazi. Ngokukhetha indlela efanele, ochwepheshe bezempilo bangahlasela futhi basuse izinto ezithile ukuze bathuthukise ubumsulwa bezingxenye ezihlukanisiwe.

Enye indlela enjalo i-density gradient centrifugation. Le ndlela isebenzisa isisombululo esinama-gradient anobukhulu obuhlukahlukene. Ngemva kwe-centrifugation, izingxenye ezahlukene zesampula yegazi zihlala ezingqimbeni ezihlukile, lapho ukungcola kutholakala ezindaweni ezithile. Le ndlela isebenza kahle kakhulu ekuhlukaniseni nasekususeni izinto ezingafuneki, ikakhulukazi amagciwane nezinhlobo ezithile zamaprotheni.

Enye indlela i-differential centrifugation, encike emazingeni ahlukene okuthuthwa kwezakhi zegazi ezahlukahlukene. Ngokulawula ngokucophelela ijubane nesikhathi se-centrifuge, ochwepheshe bezempilo bangakwazi ukuhlukanisa izingxenye ezithile ngokukhetha ngenkathi beshiya ukungcola okungafuneki.

Ukusetshenziswa Kwezokwelapha Kwe-Centrifugation Ekususeni Ukungcola Kwegazi

I-Centrifugation isetshenziswa kakhulu ezindaweni zezokwelapha ukuqinisekisa ukuhlolwa okunembile nokuthembekile. Emalabhorethri okuxilonga, kubalulekile ukuthola i-plasma noma i-serum engangcolisiwe ukuze kuhlolwe izinhlobo ezahlukene, njengokuhlaziywa kwamakhemikhali egazi, ukuhlolwa kwama-hormone, kanye nokuhlolwa kwe-immunological.

Ngaphezu kwalokho, i-centrifugation idlala indima ebalulekile ezinqubweni zokwelapha ezifana nokumpontshelwa igazi. Ngaphambi kokumpontshelwa igazi, kubalulekile ukuhlukanisa izingxenye ezifunwayo, njengamaseli abomvu egazi noma ama-platelet, nezinto ezingafuneki. I-centrifugation yenza kube lula ukuhlukaniswa kwalezi zingxenye, okuqinisekisa ukuthi umamukeli uthola ingxenye yegazi edingekayo ngaphandle kokungcola.

Ekuphetheni, i-centrifugation iyithuluzi elinamandla elisetshenziselwa ukuhlukanisa izinto ezingcolisayo kumasampula egazi. Ngokusebenzisa amasu afanele kanye nokwenza ngcono isivinini nesikhathi se-centrifuge, ochwepheshe bezempilo bangathola izingxenye ezihlanzekile zokuxilonga okunembile, ucwaningo, kanye nezinqubo zokwelapha. Ukuqonda indima ye-centrifugation ekuhlukaniseni izinto ezingcolisayo kubalulekile ekuqinisekiseni ukuthembeka nokusebenza kahle kokuhlaziywa kwezokwelapha kanye nokwelashwa.