Ke lintlha life tse silafatsang tse arohantsoeng ke centrifuge ea mali

Sehlooho

1. Ho Utloisisa Karolo ea Centrifugation ho Aroleng Lintho tse Silafatsang

2. Mefuta e fapaneng ea Litšila tsa Mali tse Tlositsoeng ka ho Centrifugation

3. Bohlokoa ba Lebelo le Lekaneng la Sentifuge le Nako bakeng sa Karohano e Atlehang

4. Mekhoa ea ho Tlosa Litšila Tse Ikhethang ka ho Centrifugation

5. Litšebeliso tsa Kliniki tsa Centrifugation ho Tlosang Litšila tsa Mali

Ho Utloisisa Karolo ea Centrifugation ho Aroleng Lintho tse Silafatsang

Ho thiba mali ho sebelisoa haholo mafapheng a fapaneng, ho kenyeletsoa bongaka le lipatlisiso, bakeng sa ho arola likarolo tse fapaneng tsa mokelikeli. Lefapheng la bongaka, ho thiba mali ho phetha karolo ea bohlokoa ho aroleng lintho tse silafatsang le lintho tse ling tse sa batleheng ho tsoa sampole ea mali. Ts'ebetso ena e nolofalletsa litsebi tsa tlhokomelo ea bophelo ho fumana likarolo tse hloekileng bakeng sa tlhahlobo, lipatlisiso le merero ea kalafo. Sehloohong sena, re tla hlahloba lintlha tse fapaneng tse ka silafatsang mali le kamoo ho thiba mali ho thusang kateng karohanong ea tsona ho netefatsa liphetho tse nepahetseng le tse tšepahalang.

Mefuta e fapaneng ea Litšila tsa Mali tse Tlositsoeng ka ho Centrifugation

Mali ke mokelikeli o rarahaneng o nang le likarolo tse ngata tse fapaneng, ho kenyeletsoa lisele tse khubelu le tse tšoeu tsa mali, li-platelet, plasma le lintho tse fapaneng tsa biochemical. Leha ho le joalo, a ka boela a ba le litšila tse sitisang liteko le tlhahlobo e nepahetseng. Ho centrifugation ho ka tlosa litšila tsena ka katleho, ho netefatsa bohloeki ba likarolo tse arohaneng.

E 'ngoe ea lintho tse silafatsang mali tse tloaelehileng ke lithōle tsa sele. Nakong ea ho bokelloa kapa ho tšoaroa, lisele tse khubelu tsa mali le lisele tse tšoeu tsa mali li ka lla, tsa lokolla litaba tsa tsona 'me tsa ama botšepehi ba sampole. Ho kenya mali ka har'a centrifuge ka lebelo le nako e itseng ho arola lithōle tsa sele, ho lumella tlhahlobo e hlakileng le e nepahetseng ea sampole.

Tšilafalo e 'ngoe e kholo ke maqeba a fibrin. Hangata lisampole tsa mali li na le fibrinogen, protheine e ikarabellang bakeng sa ho hoama mali. Ha fibrinogen e sa hlokomeloe, e ka etsa maqeba, e leng se etsang hore ho be thata hore litsebi tsa tlhokomelo ea bophelo li fumane sampole e hlakileng ea plasma. Ho chencha maqeba ho thusa ho tlosa maqeba ana, e leng se lumellang ho aroloa ha plasma e sa silafatsoang.

Bohlokoa ba Lebelo le Lekaneng la Sentifuge le Nako bakeng sa Karohano e Atlehang

Ho netefatsa karohano e sebetsang hantle ea lintho tse silafatsang, lebelo le nako e nepahetseng ea centrifuge lia hlokahala. Lebelo la centrifuge le tsamaellana le matla a khoheli a sebelisoang sampole, ha nako e tsamaellana le nako ea centrifuge.

Lebelo le nako e sa lekaneng ea ho centrifuge li ka fella ka karohano e sa lekaneng, e leng se lebisang boteng ba lintho tse silafatsang likarolong tse arohaneng. Ka lehlakoreng le leng, lebelo le nako e feteletseng li ka baka tšenyo e sa batleheng likarolong tse lakatsehang tsa mali 'me li ka lebisa liphethong tse fosahetseng tsa liteko.

Mekhoa ea ho Tlosa Litšila Tse Ikhethang ka ho Centrifugation

Ho centrifugation ho phetha karolo ea bohlokoa ho tloseng litšila tse itseng lisampoleng tsa mali. Ka ho khetha mokhoa o loketseng, litsebi tsa tlhokomelo ea bophelo li ka shebana le ho felisa lintho tse itseng ho ntlafatsa bohloeki ba likarolo tse arohaneng.

Mokhoa o mong o joalo ke density gradient centrifugation. Mokhoa ona o sebelisa tharollo e nang le gradients ea densitysities e fapaneng. Kamora centrifugation, likarolo tse fapaneng tsa sampole ea mali li lula likarolong tse fapaneng, 'me litšila li fumaneha libakeng tse itseng. Mokhoa ona o sebetsa hantle haholo ho aroleng le ho tloseng lintho tse sa batleheng, haholo-holo livaerase le mefuta e itseng ea liprotheine.

Mokhoa o mong ke ho arola ka tsela e fapaneng, e itshetlehileng hodima sedimentation e fapaneng ya dikarolo tse fapaneng tsa madi. Ka ho laola ka hloko lebelo le nako ya ho arola ka tsela e itseng, ditsebi tsa tlhokomelo ya bophelo di ka arola dikarolo tse itseng ka tsela e ikgethileng ha ka nako e le nngwe di siya ditshila tse sa batleheng.

Litšebeliso tsa Kliniki tsa Centrifugation ho Tlosang Litšila tsa Mali

Ho centrifugation ho sebediswa haholo dibakeng tsa bongaka ho netefatsa diteko tse nepahetseng le tse tshepahalang. Dilaboratoring tsa tlhahlobo ya mafu, ho bohlokwa ho fumana plasma kapa serum e sa silafatswang bakeng sa diteko tse fapaneng, tse kang tlhahlobo ya khemistri ya madi, diteko tsa dihormone, le diphuputso tsa sesole sa mmele.

Ho feta moo, ho thiba mali ho phetha karolo ea bohlokoa mekhoeng ea phekolo e kang ho tšeloa mali. Pele ho tšeloa mali, ho bohlokoa ho arola likarolo tse lakatsehang, tse kang lisele tse khubelu tsa mali tse pakiloeng kapa li-platelet, ho lintho tse sa batleheng. Ho thiba mali ho nolofalletsa ho aroloa ha likarolo tsena, ho netefatsa hore moamoheli o fumana karolo ea mali e hlokahalang ntle le litšila.

Qetellong, ho centrifugation ke sesebelisoa se matla se sebelisetsoang ho arola lintho tse silafatsang ho tsoa lisampoleng tsa mali. Ka ho sebelisa mekhoa e nepahetseng le ho ntlafatsa lebelo le nako ea ho centrifuge, litsebi tsa tlhokomelo ea bophelo li ka fumana likarolo tse hloekileng bakeng sa tlhahlobo e nepahetseng, lipatlisiso le mekhoa ea phekolo. Ho utloisisa karolo ea ho centrifugation ho aroleng lintho tse silafatsang ho bohlokoa bakeng sa ho netefatsa botšepehi le bonnete ba litlhahlobo tsa bongaka le kalafo.