zeziphi izinto ezingcolisayo ezahlulwe yi-centrifuge yegazi
Inqaku
1. Ukuqonda Indima yeCentrifugation ekwahlukaniseni izinto ezingcolisayo
2. Iintlobo ezahlukeneyo zeZingcolisi zeGazi ezisuswa yiCentrifugation
3. Ukubaluleka kwesantya esaneleyo seCentrifuge kunye nexesha lokwahlukana okusebenzayo
4. Iindlela zokususa izinto ezithile ezingcolisayo nge-Centrifugation
5. Ukusetyenziswa kweCentrifugation kwiKliniki ekuSuseni iiNgcoliso zeGazi
Ukuqonda Indima yeCentrifugation ekwahlukaniseni izinto ezingcolisayo
I-Centrifugation isetyenziswa kakhulu kwiinkalo ezahlukeneyo, kuquka amayeza nophando, ukwahlula izinto ezahlukeneyo zolwelo. Kwicandelo lezonyango, i-blood centrifugation idlala indima ebalulekileyo ekwahlukaniseni izinto ezingcolisayo kunye nezinye izinto ezingafunekiyo kwisampulu yegazi. Le nkqubo ivumela iingcali zezempilo ukuba zifumane izinto ezicocekileyo zokuxilonga, uphando, kunye neenjongo zonyango. Kweli nqaku, siza kuhlola izinto ezahlukeneyo ezinokungcolisa igazi kunye nendlela i-centrifugation enceda ngayo ekuhlukaniseni kwazo ukuqinisekisa iziphumo ezichanekileyo nezithembekileyo.
Iintlobo ezahlukeneyo zeZingcolisi zeGazi ezisuswa yiCentrifugation
Igazi lulwelo oluntsonkothileyo oluthwala uluhlu olubanzi lwezinto ezisetyenziswayo, kuquka iiseli zegazi ezibomvu nezimhlophe, iiplatelets, iplasma, kunye nezinto ezahlukeneyo zebhayoloji. Nangona kunjalo, linokuba nezinto ezingcolisayo eziphazamisa uvavanyo nohlalutyo oluchanekileyo. Ukucoca nge-centrifugation kunokususa ngokufanelekileyo ezi zinto zingcolisayo, kuqinisekisa ubunyulu bezinto ezisetyenziswayo.
Enye yezinto ezingcolisa igazi eziqhelekileyo yinkunkuma yeseli. Ngexesha lokuqokelelwa okanye ukuphathwa, iiseli ezibomvu zegazi kunye neeseli ezimhlophe zegazi zinokucoca igazi, zikhuphe umxholo wazo kwaye zichaphazele ukuthembeka kwesampuli. Ukufaka igazi kwindawo enye ngesantya esithile kunye nobude bexesha kwahlula inkunkuma yeseli, okuvumela uvavanyo olucacileyo noluchanekileyo lwesampuli.
Esinye isingcolisi esikhulu ngamahlwili e-fibrin. Iisampuli zegazi zihlala ziqulathe i-fibrinogen, iproteni enoxanduva lokujiya kwegazi. Xa ishiywe ingajongwanga, i-fibrinogen inokwenza amahlwili, nto leyo eyenza kube nzima kwiingcali zezempilo ukufumana isampuli ye-plasma ecacileyo. Ukucoca nge-centrifugation kunceda ekususweni kwala mahlwili, okuvumela ukwahlulwa kwe-plasma engangcoliswanga.
Ukubaluleka kwesantya esaneleyo se-Centrifuge kunye nexesha lokwahlukana okusebenzayo
Ukuqinisekisa ukwahlulahlula okusebenzayo kwezinto ezingcolisayo, isantya esifanelekileyo se-centrifuge kunye nexesha zibalulekile. Isantya se-centrifuge sihambelana namandla omxhuzulane asetyenziswa kwisampuli, ngelixa ixesha lihambelana nobude be-centrifugation.
Isantya kunye nexesha elaneleyo lokujika i-centrifuge kunokubangela ukwahlukana okunganelanga, okukhokelela ekubeni kubekho izinto ezingcolisayo kwiindawo ezizimeleyo. Ngokwahlukileyo koko, isantya kunye nexesha eligqithisileyo kunokubangela umonakalo ongafunekiyo kwiindawo zegazi ezifunwayo kwaye kunokukhokelela kwiziphumo zovavanyo ezingachanekanga.
Iindlela zokususa izinto ezithile ezingcolisayo nge-Centrifugation
Ukucoca igazi nge-centrifugation kudlala indima ebalulekileyo ekususeni ungcoliseko oluthile kwiisampuli zegazi. Ngokukhetha indlela efanelekileyo, iingcali zonyango zinokujolisa kwaye zisuse izinto ezithile ukuphucula ubunyulu bezinto ezikhethiweyo.
Enye indlela enjalo yi-density gradient centrifugation. Le ndlela isebenzisa isisombululo esine-gradients zobuninzi obahlukeneyo. Emva kwe-centrifugation, iinxalenye ezahlukeneyo zesampulu yegazi zihlala kwiileya ezahlukeneyo, kunye nongcoliseko olufumaneka kwiindawo ezithile. Le ndlela isebenza kakuhle kakhulu ekwahlukaniseni nasekususeni izinto ezingafunekiyo, ngakumbi iintsholongwane kunye neentlobo ezithile zeeproteni.
Enye indlela kukusebenzisa i-differential centrifugation, exhomekeke kumazinga ahlukeneyo okutsalwa kwe-sedimentation kwiinxalenye ezahlukeneyo zegazi. Ngokulawula ngononophelo isantya kunye nexesha le-centrifuge, iingcali zezempilo zinokwahlulahlula ngokukhetha iinxalenye ezithile ngelixa zishiya ngasemva izinto ezingafunekiyo.
Ukusetyenziswa kweCentrifugation kwiKliniki ekuSuseni iiNgcoliso zeGazi
I-Centrifugation isetyenziswa kakhulu kwiindawo zonyango ukuqinisekisa uvavanyo oluchanekileyo noluthembekileyo. Kwiilabhoratri zokuxilonga, kubalulekile ukufumana i-plasma okanye i-serum engangcoliswanga kwiimvavanyo ezahlukeneyo, ezinje ngohlalutyo lwekhemistri yegazi, uvavanyo lweehomoni, kunye nophando lwe-immunological.
Ngaphezu koko, i-centrifugation idlala indima ebalulekileyo kwiinkqubo zonyango ezifana notofelo-gazi. Ngaphambi kotofelo-gazi, kubalulekile ukwahlula izinto ezifunekayo, ezifana neeseli ezibomvu zegazi ezipakishiweyo okanye iiplatelets, kwizinto ezingafunekiyo. I-centrifugation ivumela ukwahlula ezi zinto, ukuqinisekisa ukuba umntu ofumana igazi ufumana into efunekayo ngaphandle kokungcola.
Ukuqukumbela, i-centrifugation sisixhobo esinamandla esisetyenziselwa ukwahlula izinto ezingcolisayo kwiisampuli zegazi. Ngokusebenzisa iindlela ezifanelekileyo kunye nokwenza ngcono isantya kunye nexesha le-centrifuge, iingcali zezempilo zinokufumana izinto ezicocekileyo zokuxilongwa ngokuchanekileyo, uphando, kunye neenkqubo zonyango. Ukuqonda indima ye-centrifugation ekwahlukaniseni izinto ezingcolisayo kubalulekile ekuqinisekiseni ukuthembeka kunye nokusebenza kohlalutyo lweklinikhi kunye nonyango.
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