igazi lihlukana kanjani ku-centrifuge

Igazi Lihlukana Kanjani Ku-Centrifuge?

Isingeniso:

Ama-centrifuge ayizinto ezinamandla zelebhu ezisetshenziselwa ukuhlukanisa izingxenye ezahlukene zengxube ngamandla e-centrifugal. Anezinhlobo eziningi zezicelo ocwaningweni lwezinto eziphilayo, amakhemikhali, kanye nolwezokwelapha. Enye yezindlela ezibaluleke kakhulu zokusebenzisa ama-centrifuge ukuhlukaniswa kwezingxenye zegazi. Le nqubo idlala indima ebalulekile ezinqubweni ezahlukene zezokwelapha, okuhlanganisa ukumpontshelwa igazi, ukuxilongwa kwezifo, kanye nocwaningo. Kulesi sihloko, sizohlola ukuthi igazi lihlukana kanjani ku-centrifuge kanye nokubaluleka kwale nqubo kwezokwelapha zanamuhla.

I. Ukuqonda Izingxenye Zegazi:

Ngaphambi kokuhlola indlela yokuhlukanisa, ake siqonde kafushane izingxenye zegazi. Igazi lakhiwa amangqamuzana abomvu egazi, amangqamuzana amhlophe egazi, ama-platelet, kanye ne-plasma. Amangqamuzana abomvu egazi athutha umoya-mpilo kuwo wonke umzimba, amangqamuzana amhlophe egazi adlala indima ebalulekile ekuphenduleni komzimba, ama-platelet asiza ekujiyeni kwegazi, kuyilapho i-plasma iyisakhi esiwuketshezi esithwala izakhamzimba, imikhiqizo engcolile, ama-hormone, kanye namaprotheni.

II. Inqubo Yokucisha Ukushisa:

I-Centrifugation isebenzisa isimiso se-sedimentation, lapho izinhlayiya ezixineneyo zihamba khona ziphume zihlale phansi ngenxa yamandla e-centrifugal. Ukuhlukaniswa kwegazi kuhilela ukubeka isampula yegazi epayipini elikhethekile elaziwa ngokuthi ipayipi le-centrifuge. Leli payipi libe selifakwa emshinini we-centrifuge, ojikeleza ngesivinini esikhulu, ukhiqize amandla e-centrifugal adingekayo.

III. Isinyathelo 1: Ukukhiqizwa Kwamandla E-Centrifugal:

Njengoba umshini we-centrifuge uqhubeka ngamandla, ukhiqiza amandla e-radial, aziwa ngokuthi amandla e-centrifugal. La mandla asunduza izingxenye zegazi ziphume zisuka enkabeni yokujikeleza. Izingxenye ezisindayo, njengamaseli abomvu egazi nama-platelet, zihlala eduze kwezansi, kuyilapho izingxenye ezilula, okuhlanganisa ne-plasma, zihamba ziye phezulu.

IV. Isinyathelo 2: Ukwakheka Kwezendlalelo:

Ngenxa yobuningi obuhlukene bezingxenye zegazi, ziyahlukana zibe yizingqimba ezihlukene ngaphakathi kwepayipi le-centrifuge. Ingxenye esindayo kakhulu, amaseli egazi abomvu, yakha ungqimba olungezansi, olulandelwa ungqimba oluncane lwama-platelet, olubizwa ngokuthi i-buffy coat. Ungqimba oluphezulu luqukethe i-plasma, ebonakala njengoketshezi oluphuzi.

V. Isinyathelo 3: Ukuqinisa Nokuqoqwa:

Uma inqubo yokuhlukanisa isiqediwe, i-centrifuge iyamiswa, bese kuthi ipayipi le-centrifuge lisuswe ngokucophelela ukuze kuvinjelwe ukuthi izingqimba zingaxubani. Izingqimba ezizinzile zezingxenye zegazi zingaqoqwa ngezindlela ezahlukene, njengokufaka amapayipi noma ukukhipha amanzi, kuye ngokuthi kuhloswe ukusetshenziswa ini.

VI. Izici Ezithinta Ukuhlukaniswa Kwegazi:

Izici eziningana zithonya ukusebenza kahle nokunemba kokuhlukaniswa kwegazi ku-centrifuge. Lezi zici zifaka phakathi ijubane nobude be-centrifuge, ukwakheka nosayizi wamashubhu e-centrifuge asetshenziswayo, kanye nohlobo lomshini we-centrifuge. Kubalulekile ukwenza ngcono lezi zinhlaka ukuze kufezwe imiphumela oyifunayo futhi kugwenywe noma yikuphi ukulimala kwezingxenye zegazi.

VII. Ukubaluleka Kwezicelo Zezokwelapha:

Ukuhlukaniswa kwegazi ku-centrifuge kubaluleke kakhulu ezindleleni ezahlukene zokwelapha:

1. Ukumpontshelwa Igazi: Ukumpontshelwa igazi kusiza ukuhlukanisa igazi libe izingxenye zalo, okuvumela ukukhethwa kwezingxenye ezithile zegazi ezidingekayo ukuze kumpontshelwe igazi. Isibonelo, inqubo yokuhlukaniswa ingahlukanisa amangqamuzana abomvu egazi ezigulini ezintula igazi noma ama-platelet kubantu abanezinkinga zokujiya kwegazi.

2. Ukuxilongwa Kwesifo: Ngokuhlukanisa izingxenye zegazi, ochwepheshe bezokwelapha bangahlaziya izingxenye ezithile ukuze bathole izimpawu zesifo kanye nokukhubazeka. Lokhu kusiza ekuxilongweni kwezimo ezifana ne-anemia, izifo ezithelelanayo, i-leukemia, nokuningi.

3. Ucwaningo Nokuthuthukiswa Kwemithi: Ukuhlukaniswa kwegazi kudlala indima ebalulekile ocwaningweni lwelabhorethri. Ososayensi bangathola izingxenye ezihlanziwe njengezingqamuzana zegazi ezimhlophe ukuze batadishe izimpendulo zomzimba, bahlaziye amaprotheni e-plasma, noma bahlole umsebenzi wama-platelet. Lezi zifundo zinegalelo ekwakheni imithi, imijovo, kanye nokuqonda izifo ezahlukahlukene.

4. Izinqubo Zokwelapha: I-Centrifugation isekela izinqubo zokwelapha ezahlukahlukene, njengokwelashwa ngamaseli e-stem. Ngokuhlukanisa izingxenye ezithile ezifana namaseli e-stem egazini lesiguli, zingasetshenziswa ukusiza ukuvuselela izicubu ezonakele nokwelapha izimo ezithile.

5. Ukugcinwa Kwegazi: Emabhange egazi, ukususwa kwegazi kuvumela ukuhlukaniswa kwegazi eliphelele libe izingxenye zalo, okwenza kube lula ukugcinwa okuphephile nokutholakala kwezingxenye ezithile njengoba kudingeka. Lokhu kwenza kube lula ukuphathwa kwegazi okuphumelelayo futhi kuqinisekisa ukuhambisana ngesikhathi sokumpontshelwa igazi.

Isiphetho:

Ukwehlukanisa igazi nge-centrifugation kuyinqubo ebalulekile kwezokwelapha zanamuhla evumela ukuhlukaniswa nokuhlukaniswa kwezingxenye zegazi. Ngokuqonda ukuthi igazi lihlukana kanjani nge-centrifuge, singaqonda ukubaluleka kwalo ezindleleni ezahlukene zezokwelapha, okuhlanganisa ukumpontshelwa igazi, ukuxilongwa kwezifo, ucwaningo, kanye nezinqubo zokwelapha. Le ndlela enamandla iyaqhubeka nokufaka isandla ekuthuthukisweni kwezempilo kanye nokuthuthukisa imiphumela yeziguli.