ingaba i-centrifuge ingazahlula izinto ezibotshelelwe ngeekhemikhali
Ngaba iCentrifuge ingazahlula izinto ezidityaniswe neekhemikhali?
Intshayelelo:
I-Centrifugation yindlela esetyenziswa kakhulu kwiilabhoratri, ikakhulu ukwahlula imixube ngokuyijikeleza ngesantya esiphezulu. Ibonakalise ukuba iyasebenza ekwahlukaniseni imixube eyenziwe ngamanqanaba ahlukeneyo, njengezinto ezimanzi kunye nezinto eziqinileyo. Nangona kunjalo, umntu unokuzibuza ukuba i-centrifuge ingazahlula na izinto ezibotshelelwe ngamakhemikhali. Eli nqaku lihlola amathuba kunye nemida ye-centrifugation xa kufikwa ekuqhekezeni imixube yeekhemikhali kunye nokwahlula izinto ezibotshelelweyo.
1. Ukuqonda i-Centrifugation:
Ngaphambi kokuba singene kumbuzo okhoyo, kubalulekile ukuqonda iziseko ze-centrifugation. I-centrifuge ine-rotor ejikeleza ngesantya esiphezulu, nto leyo ebangela ukuba umxube ongaphakathi kuyo ungene kwi-gravitational forces kwaye wahlukane ngokusekelwe kumahluko oxinano. Izinto ezixineneyo zitsalwa ezantsi, zenze i-pellet, ngelixa izinto ezixineneyo zingaxinenenga kangako zihlala kwi-supernatant.
2. Uhlobo lweeBond zeKhemikhali:
Iibhondi zekhemikhali ngamandla adibanisa iiathomu ukuze zenze iimolekyuli kunye neekhompawundi. Ezi bondi zinokuba yi-covalent, i-ionic, okanye i-metallic, kuxhomekeke kuhlobo lweeathomu ezibandakanyekayo. Iibhondi ze-covalent ziquka ukwabelana ngee-electron, ngelixa iibhondi ze-ionic zivela ekudlulisweni kwee-electron ukusuka kwi-athomu enye ukuya kwenye. Iibhondi ze-metallic zenzeka xa ii-electron zabelwana ngazo kwiiathomu ezininzi kwi-lattice yesinyithi.
3. Imida yeCentrifugation yokuQhekeza iiBond zeKhemikhali:
I-centrifuge yenzelwe ukwahlula imixube ngokusekelwe kumahluko oxinano, kungekhona ukuqhekeza ii-chemical bonds. Ii-chemical bonds zinamandla amakhulu, afuna amandla amaninzi ukuze aphulwe. Amandla ajikelezayo aveliswa yi-centrifuge ngokubanzi awanele ukoyisa la mandla e-bond. Ke ngoko, kunzima ukusebenzisa i-centrifugation yodwa ukwahlula izinto ezibotshelelwe ngeekhemikhali.
4. Ukusebenzisa Iindlela Zekhemikhali Nezomzimba:
Nangona i-centrifuge isenokungasebenzi yodwa, isenokudlala indima ebalulekileyo xa idibene nezinye iindlela zokwahlula izinto ezidityaniswe ngeekhemikhali. Ngokudibanisa iindlela zeekhemikhali nezendalo, amandla e-bond anokuthotywa okanye aphuke, nto leyo ebenza bakwazi uku-centrifugation. Ezinye iindlela eziqhelekileyo ezisetyenziswa kunye ne-centrifugation ziquka ukukhupha i-solvent, ukugaya i-enzyme, kunye nokuchithwa kobushushu okanye kweekhemikhali.
5. Ukukhupha iSolvent:
Ukukhupha i-solvent yindlela esebenzisa i-solvents ukunyibilikisa enye inxalenye yomxube ngelixa ezinye zishiya zinjalo. Ngokukhetha i-solvent enyibilikisa ngokukhetha enye yezinto ezidityaniswe neekhemikhali, kunokwenzeka ukuba buthathaka okanye ukwaphule ii-bond. Emva kokukhupha, i-centrifuge ingasetyenziselwa ukwahlula izinto ezidityanisiweyo ezine-solvent kwizinto ezisele zomxube.
6. Ukugaya ukutya nge-Enzymatic:
Ukugaya ukutya nge-enzyme kubandakanya ukusetyenziswa kwee-enzyme ezithile ezinokuqhekeza iibhondi zeekhemikhali kwiimolekyuli zebhayoloji. Ngokongeza ii-enzyme ezifanelekileyo kumxube ngezinto ezibotshelelwe ngeekhemikhali, iibhondi zinokufakwa kwi-hydrolyzed okanye ziqhekeke nge-enzyme, nto leyo enciphisa amandla azo. Umxube ophumayo ungafakwa kwi-centrifugation ukuze kwahlulwe iinxalenye ngobuninzi bazo.
7. Ukubola kobushushu okanye kweekhemikhali:
Kwezinye iimeko, ukufaka izinto ezidityaniswe ngeekhemikhali kumaqondo obushushu aphezulu okanye kwiikhemikhali ezidilikayo kunokwenza buthathaka okanye kwaphule iibond. Umzekelo, ukufudumeza umxube kunokubangela ukubola kobushushu, okukhokelela ekuqhekekeni kweekhemikhali ezithile. Emva konyango lobushushu okanye lwekhemikhali, i-centrifuge ingasetyenziselwa ukwahlula umxube ophumayo ngokusekelwe kumahluko oxinano.
Isiphelo:
Nangona i-centrifuge ngokwayo isenokungakwazi ukuphula iibhondi zeekhemikhali, isenokuba sisixhobo esibalulekileyo ekwahlukaniseni izinto ezidityaniswe neekhemikhali. Ukudibanisa i-centrifugation neendlela ezifana nokukhupha i-solvent, ukugaywa kwe-enzyme, okanye ukuchithwa kobushushu kunye neekhemikhali kunokwenza buthathaka okanye kuphule iibhondi ngempumelelo, kuzenze zilungele ukwahlukana ngokusekelwe kumahluko woxinano. Ngokuqonda imida kunye namathuba e-centrifugation, izazinzulu zingayisebenzisa kunye nezinye iindlela zokufezekisa ukwahlukana ngempumelelo kwezinto ezidityaniswe neekhemikhali.
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