Ke mefuta efe ea metsoako e arotsoeng ka centrifugation

Ho Centrifugation: Ho Arola Metswako e Fapaneng ho Thehilwe hodima Dipharologanyo tsa Botenya

Selelekela:

Ho Centrifugation ke mokhoa o matla oa laboratori o sebelisoang ho arola metsoako ho latela liphapang tsa eona tsa bongata. Ka ho beha motsoako tlas'a potoloho e potlakileng, ho centrifugation ho sebelisa melao-motheo ea matla a khoheli ho fihlela karohano. Mokhoa ona o fumana lits'ebetso tse pharaletseng masimong a fapaneng, ho kenyeletsoa baeloji, k'hemistri, bongaka le tse ling tse ngata. Sehloohong sena, re tla hlahloba mefuta e fapaneng ea metsoako e ka aroloang ka katleho ka ho centrifugation, hammoho le lits'ebetso tsa eona tse fapaneng.

1. Karohano ea Likarolo tsa Mali:

E 'ngoe ea lits'ebetso tse tloaelehileng tsa ho centrifugation ke karohano ea likarolo tsa mali. Mali a felletseng a entsoe ka likarolo tse fapaneng tsa sele le tsa mokelikeli, e 'ngoe le e 'ngoe e na le bongata bo fapaneng. Ka ho potoloha sampole ea mali ka har'a centrifuge, likarolo tsena li ka aroloa ka mekhahlelo e fapaneng. Likarolo tse boima, joalo ka lisele tse khubelu tsa mali, li lula tlase, ha tse bobebe, joalo ka plasma kapa serum, li sala holimo.

Ho centrifugation ho thusa ho arola dikarolo tsa madi bakeng sa merero ya ho hlahloba, jwalo ka ho fumana maemo a hematocrit kapa ho arola disele tse itseng jwalo ka diplatelete kapa disele tse tshweu tsa madi bakeng sa tlhahlobo e eketsehileng. Ho feta moo, ho centrifugation ho bapala karolo ya bohlokwa ho bankeng ya madi, ho nolofalletsa ho arolwa ha madi dikarolong tse fapaneng bakeng sa merero ya tshelo.

2. Ho hlwekisa DNA le RNA:

Mekhoa ea ho ntša DNA le RNA e itšetlehile haholo ka ho centrifugation bakeng sa tlhoekiso e sebetsang hantle. Nakong ea ts'ebetso ea ho ntša, lisampole tsa baeloji hangata li na le litšila, tse kang liprotheine, lipids, kapa lithōle tse ling tsa sele, tse ka sitisang tlhahlobo ea liphatsa tsa lefutso e latelang. Ho centrifugation ho thusa ho arola DNA kapa RNA ho litšila tsena, e leng se fellang ka thepa ea liphatsa tsa lefutso e hloekisitsoeng haholo.

Mekhoa e fapaneng ea ho centrifugation, joalo ka ho centrifugation e fapaneng le ho centrifugation ea gradient ea isopycnic density, e sebelisoa ho arola DNA kapa RNA ho latela bongata ba tsona. Ts'ebetso ena ea tlhoekiso e bohlokoa bakeng sa lits'ebetso tse kang ho latellana ha liphatsa tsa lefutso, PCR (polymerase chain reaction), le ho hatisa menoana ea DNA, kaha e netefatsa liphetho tse nepahetseng le tse tšepahalang.

3. Karohano ea Lintho tse nyenyane Tlhahlobong ea Tikoloho:

Ho centrifugation ho boetse ho fumana ts'ebeliso ea eona tlhahlobong ea tikoloho, haholo-holo karohanong le tlhahlobong ea lintho tse nyenyane. Lisampole tsa tikoloho, tse kang sediment kapa mobu, li ka ba le mefuta e fapaneng ea likaroloana tsa tlhaho le tse sa pheleng tse nang le bongata bo fapaneng. Ka ho beha lisampole tsena tlas'a centrifugation, bahlahlobi ba ka arola likarolo tse fapaneng ho latela sekhahla sa tsona sa sedimentation.

Mokhoa ona oa karohano o bohlokoa bakeng sa ho ithuta litšila tsa tikoloho, ho khetholla litšepe tse boima, kapa ho sekaseka kabo ea likaroloana lisampoleng tsa moea kapa tsa metsi. Ho sebelisa centrifugation ho thusa ho arola likaroloana tse itseng tsa lintho tse nyenyane, ho lumella tlhahlobo e nepahetseng le maano a latelang a ho fokotsa lintho.

4. Ho aroloa ha Li-organelle tse ka tlas'a lisele:

Ho centrifugation ke mokhoa oa bohlokoahali bakeng sa ho ithuta meaho ea sele le mesebetsi ea eona. Ka ho sebelisa differential centrifugation kapa density gradient centrifugation, bafuputsi ba ka khetholla li-organelle tse itseng tsa subcellular, tse bohlokoa bakeng sa ho utloisisa lits'ebetso tsa sele.

Mohlala, mitochondria, e leng matlo a matla a sele, e ka ruisoa le ho aroloa ho likarolo tse ling tsa sele ka ho etsa ultracentrifugation. Ka ho tšoanang, li-organelle tse ling tse kang lysosomes, peroxisomes, kapa endoplasmic reticulum li ka aroloa ka ho etsa centrifugation e fapaneng ka lebelo le bolelele bo fapaneng.

5. Tlhoekiso ea Likaroloana tsa Vaerase:

Ho centrifugation ho bapala karolo ea bohlokoa ho virology, haholo-holo ho hloekiseng likaroloana tsa vaerase. Livaerase li tsebahala ka boholo ba tsona bo bonyenyane le ho rarahana ha tsona, e leng se etsang hore ho itšehla thajana ha tsona e be mosebetsi o boima. Leha ho le joalo, ka ho sebelisa mekhoa ea ho centrifugation e potlakileng joalo ka ultracentrifugation, bafuputsi ba ka arola likaroloana tsa vaerase ho lisele tse amohelang le litšila tse ling.

Ts'ebetso ena ea tlhoekiso e thusa ho utloisisa sebopeho sa vaerase, ho batlisisa tšoaetso ea vaerase le ho nts'etsapele liente. Karohano ka ho sebelisa centrifugation e boetse e thusa ho lekanya vaerase 'me e nolofalletsa tlhahlobo e eketsehileng, ho kenyeletsoa le microscopy ea elektronike kapa tlhaloso ea limolek'hule.

Qetello:

Ho sebelisa centrifugation ke mokhoa o feto-fetohang o nolofalletsang karohano ea metsoako e fapaneng ho latela liphapang tsa eona tsa bongata. Ts'ebeliso ea eona e pharaletseng, ho tloha tlhahlobong ea bongaka le tlhahlobo ea liphatsa tsa lefutso ho ea lipatlisisong tsa tikoloho le virology. Ka ho sebelisa melao-motheo ea matla a khoheli le ho potoloha ka lebelo le phahameng, ho sebelisa centrifugation ho tsoela pele ho ba sesebelisoa sa bohlokoa boitekong bo bongata ba saense.