mefuta e mekae ea mochini oa centrifuge

Mechini ea Centrifuge: Tataiso e Felletseng ea Mefuta le Litšebeliso tse Fapaneng

Selelekela

Mechini ea centrifuge ke lisebelisoa tsa bohlokoa tsa laboratori tse sebelisetsoang ho arola lintho tse metsi le tse tiileng ho latela phapang ea tsona ea bongata. Li fumana lits'ebetso tse pharaletseng liindastering tse fapaneng, ho kenyeletsoa tlhokomelo ea bophelo bo botle, lipatlisiso, meriana le saense ea tikoloho. Sengoloa sena se fana ka kakaretso e felletseng ea mefuta e fapaneng ea mechini ea centrifuge, e totobatsang likarolo tsa eona tse ikhethang, lits'ebetso le melemo.

I. Metheo ea Mechini ea Centrifuge

Mechini ea centrifuge e sebetsa ho latela molao-motheo oa matla a centrifugal, moo rotor e potolohang e hlahisang tšimo e matla ea centrifugal. Matla ana a etsa hore likaroloana tse boima kapa lintho li tlohe bohareng, e leng se etsang hore li arohane le likarolo tse bobebe. Mefuta e meholo ea mechini ea centrifuge ke ena:

1.1. Di-Centrifuge tsa Angle tse Tsitsitseng

Li-centrifuge tse nang le angle e tsitsitseng ke e 'ngoe ea mefuta e tloaelehileng haholo, e entsoeng ka angle e tsitsitseng ea rotor ka kakaretso e tlohang ho likhato tse 25 ho isa ho tse 40. Li loketse ho arola boholo ba sampole e nyane 'me li fana ka sekhahla se phahameng sa ho senngoa ha sedimentation. Li-centrifuge tsena li loketse mosebetsi oa kamehla oa laboratori 'me li sebelisetsoa lits'ebetso tse kang ho ntšoa ha DNA, lipatlisiso tsa protheine le karohano ea lisele.

1.2. Li-Centrifuge tsa Libakete tse Sukang

Li-centrifuge tsa bakete tse phethohang li na le rotor ea bakete e tsamaeang e phethohang ho tloha boemong bo otlolohileng ho ea boemong bo otlolohileng nakong ea ts'ebetso. Motsamao ona o ntlafatsa ho kopanya sampole 'me o lumella karohano ea bongata bo boholo ba sampole. Li-centrifuge tsa bakete tse phethohang li sebelisoa hangata libankeng tsa mali, lilaboratoring tsa tlhahlobo ea mafu le litsi tsa lipatlisiso tsa bongaka.

1.3. Li-Ultracentrifuge

Li-centrifuge tsa Ultracentrifuge ke mechine e tsoetseng pele ea centrifuge e khonang ho fihlella lebelo le phahameng la ho potoloha, hangata e feta 100,000 RPM. Li sebelisoa haholo-holo bakeng sa ho arola limolek'hule tse kholo, livaerase le likaroloana tse ka tlas'a sele ka ho nepahala ho hoholo. Li-centrifuge tsa Ultracentrifuge li bohlokoa haholo mafapheng a lipatlisiso tsa saense joalo ka baeloji ea limolek'hule, biochemistry le virology.

II. Mechini e Ikhethileng ea Centrifuge

2.1. Di-Centrifuge tse Sehatsetsing

Li-centrifuge tse sehatsetsing li na le sistimi ea ho pholisa e hahiloeng kahare e nolofalletsang taolo e nepahetseng ea mocheso nakong ea ts'ebetso ea sampole. Li-centrifuge tsena li bohlokoa bakeng sa lits'ebetso tse hlokang mocheso o tlase, joalo ka tatellano ea DNA, tlhoekiso ea enzyme, le mosebetsi oa setso sa sele. Pholiso e laoloang e thibela ho senyeha ha sampole 'me e boloka botšepehi ba sampole.

2.2. Li-Microcentrifuge

Li-microcentrifuge ke mechine e menyenyane ea centrifuge e etselitsoeng ka ho khetheha ho sebetsana le bongata bo bonyenyane ba sampole, hangata e tloha ho 0.2 mL ho isa ho 2 mL. Li loketse lits'ebetso tse kenyeletsang li-microtubes, joalo ka ho aroloa ha DNA, ho hlophisoa ha PCR, le ho nosetsa ha protheine. Boholo ba tsona bo bonyenyane bo li etsa hore li lokele lits'ebetso tsa liteko tse nkehang habobebe le tsa tlhokomelo.

2.3. Li-Centrifuge tse Potlakileng

Li-centrifuge tse lebelo le phahameng li entsoe ho fihlella le ho boloka lebelo le phahameng la ho potoloha, ho nolofatsa linako tsa karohano tse potlakileng. Mechini ena e sebelisoa bakeng sa mefuta e mengata ea lits'ebetso, ho kenyeletsoa lipatlisiso tsa vaerase, karohano ea lisele tse ka tlase, le tlhoekiso ea nanoparticle. Li-centrifuge tse lebelo le phahameng li fana ka katleho e ntlafalitsoeng le tlhahiso ea sampole, e leng se etsang hore e be ea bohlokoa lilaboratoring tse nang le bongata bo phahameng.

III. Litšebeliso tsa Bongaka le tsa Liindasteri

3.1. Liindasteri tsa Bongaka le Tlhahlobo

Lefapheng la bongaka, mechine ea centrifuge e bohlokoa haholo bakeng sa ho sekaseka le ho hlahloba mafu a fapaneng. Hangata li sebelisoa lilaboratoring tsa kliniki bakeng sa liteko tsa mali, ho kenyeletsoa lipalo tse felletseng tsa mali, tlhahlobo ea tsoekere ea mali le karohano ea mali maling. Ho feta moo, centrifugation e bohlokoa haholo ha ho hlahlojoa maro a 'mele, joalo ka moroto le mokelikeli oa boko, e leng se thusang ho hlahloba le ho beha leihlo lefu lena.

3.2. Ntlafatso ea Meriana le Meriana

Mechini ea centrifuge e bapala karolo ea bohlokoa indastering ea meriana, e thusa ho sibolleng meriana, ho e etsa le ho laola boleng. Li sebelisetsoa lits'ebetso tse kang karohano ea metsoako e sebetsang ea meriana (API), tlhoekiso ea metsoako le liteko tsa ho e etsa. Centrifuge e thusa bo-rasaense ho fihlela karohano e nepahetseng, e tlatsetsang nts'etsopele e sebetsang ea meriana le katleho e ntlafetseng.

3.3. Saense ea Tikoloho

Mechini ea centrifuge e sebelisoa haholo saenseng ea tikoloho le lipatlisisong, haholo-holo kalafong le tlhahlobong ea metsi a litšila. Ka ho arola lintho tse tiileng tse emisitsoeng metsing a litšila, centrifuge e kenya letsoho ho tloseng litšila, e netefatsa mehloli ea metsi e hloekileng. Ho feta moo, e thusa ho sekaseka lisampole tsa mobu, ho ithuta mekhoa ea ho senyeha ha mobu, le ho beha leihlo litšila tsa tikoloho.

IV. Melemo ea Mechini ea Centrifuge

4.1. Ho Sebelisa Nako Hantle

Mechini ea centrifuge e fana ka karohano e potlakileng, e fokotsang linako tsa ts'ebetso haholo ha e bapisoa le mekhoa ea letsoho. Ho sebetsa hantle ha nako ena ho bohlokoa lits'ebetsong tse nang le nako e ngata kapa lilaboratori tse nang le tlhahiso e phahameng, e leng se nolofalletsang tlhahlobo e potlakileng le tsoelo-pele ea lipatlisiso.

4.2. Ho nepahala ho Ntlafetseng

Ho centrifugation ho fana ka diphetho tse nepahetseng haholo, ho netefatsa karohano e nepahetseng ya dikarolo tse fapaneng. Phapang e hlakileng pakeng tsa diedi le dintho tse tiileng tse fumanweng ka ho centrifugation e ntlafatsa botshepehi ba diteko tsa laboratori le diteko tsa tlhahlobo.

4.3. Ho feto-fetoha ha maemo

Mechini ea centrifuge ke lisebelisoa tse feto-fetohang tse khonang ho amohela boholo le boholo ba disampole tse fapaneng. Ka mefuta e mengata ea li-rotor le li-adapter, li ka ikamahanya le litlhoko tse fapaneng tsa lipatlisiso, e leng se etsang hore li be tsa bohlokoa lilaboratoring tse fapaneng.

4.4. Paballo ea Mohlala

Ts'ebetso e laoloang ea ho centrifugation e thusa ho boloka botšepehi ba lisampole tse bonolo. Ka ho arola likarolo ka hloko ho latela bongata, lisampole li boloka thepa ea tsona ea pele 'me li loketse tlhahlobo kapa polokelo e eketsehileng.

Qetello

Mechini ea centrifuge ke lisebelisoa tsa bohlokoa tsa laboratori tse sebelisoang liindastering tse fapaneng, tse fanang ka karohano e tšepahalang le tlhahlobo ea lintho tse fapaneng. Ho utloisisa mefuta e fapaneng ea mechini ea centrifuge e fumanehang, lits'ebetso tsa eona, le melemo e amanang le eona ho lumella bafuputsi le litsebi ho ntlafatsa ts'ebeliso ea tsona, ho ntlafatsa tlhahiso le liphetho tsa lipatlisiso.