mhando dzemuchina we centrifuge dzingangani

Michina yeCentrifuge: Gwaro Rakakwana reMarudzi Akasiyana uye Mashandisirwo

Nhanganyaya

Michina yeCentrifuge imidziyo yakakosha yemurabhoritari inoshandiswa kupatsanura zvinwiwa nezvinhu zvakasimba zvichienderana nekusiyana kwehuwandu hwazvo. Inowana mashandisirwo akapararira mumaindasitiri akasiyana-siyana, anosanganisira hutano, tsvakurudzo, mishonga, uye sainzi yezvakatipoteredza. Chinyorwa chino chinopa ongororo yakazara yemhando dzakasiyana dzemichina yeCentrifuge, ichiratidza hunhu hwayo hwakasiyana, mashandisirwo ayo, uye mabhenefiti ayo.

I. Nheyo dzeMichina yeCentrifuge

Michina yeCentrifuge inoshanda pasi pesimba reCentrifugal, uko rotor inotenderera inogadzira munda wakasimba weCentrifugal. Simba iri rinoita kuti zvidimbu kana zvinhu zvinorema zvibve pakati, zvichiita kuti zviparadzaniswe nezvikamu zvakareruka. Heano marudzi makuru emichina yeCentrifuge:

1.1. MaCentrifuge eAngle Akasimbiswa

Ma "fixed angle centrifuges" ndeimwe yemhando dzakajairika, akagadzirwa ne "fixed rotor angle" inowanzova pakati pemadhigirii makumi maviri nemashanu kusvika makumi mana. Akanakira kupatsanurwa kwemavhoriyamu madiki emuenzaniso uye anopa mwero wepamusoro wekudonha kwemvura. Ma "centrifuges" aya akakodzera basa renguva dzose murabhoritari uye anoshandiswa mukushandiswa kwakadai sekutorwa kweDNA, kutsvagurudza mapuroteni, uye kupatsanurwa kwemasero.

1.2. MaCentrifuge eBhaketi Rinotenderera

Ma "swinging bucket centrifuge" ane "movable bucket rotor" inotenderera kubva panzvimbo yakatwasuka kuenda panzvimbo yakatwasuka panguva yekushanda. Kufamba uku kunosimudzira kusanganiswa kwesampuro uye kunoita kuti huwandu hwesampuro huru huparadzaniswe. Ma "swinging bucket centrifuge" anoshandiswa zvakanyanya mumabhanga eropa, marabhoritari ekuongorora, uye masangano ekutsvaga makiriniki.

1.3. Ma "Ultracentrifuges"

Ma ultracentrifuge michina yepamusoro-soro inokwanisa kuita kumhanya kwakanyanya, kazhinji inodarika 100,000 RPM. Anonyanya kushandiswa pakuparadzanisa macromolecules, mavhairasi, uye zvidimbu zvepasi pemasero nemazvo. Ma ultracentrifuge akakosha muminda yekutsvagisa kwesainzi senge molecular biology, biochemistry, uye virology.

II. Michina yeCentrifuge yehunyanzvi

2.1. MaCentrifuge Akachengetwa muFriji

Ma centrifuge akachengetwa mufiriji ane sisitimu yekutonhodza yakavakirwa mukati iyo inobvumira kudzora tembiricha chaiyo panguva yekugadzirisa sampuro. Ma centrifuge aya akakosha pamashandisirwo anoda tembiricha yakaderera, senge DNA sequencing, kuchenesa ma enzyme, uye basa rekusimudzira masero. Kutonhodza kwakadzorwa kunodzivirira kuora kwesampuro uye kunochengetedza kunaka kwesampuro.

2.2. Microcentrifuges

Microcentrifuges michina midiki ye centrifuge yakagadzirirwa kubata zvidimbu zvidiki zvesampuru, inowanzova pakati pe 0.2 mL kusvika 2 mL. Yakanakira kushandiswa kwe microtubes, senge DNA isolation, PCR setup, uye protein precipitation. Hudiki hwayo hunoita kuti ikwanise kushandiswa mukuyedza zvinhu zvinotakurika uye munzvimbo dzinochengeterwa.

2.3. MaCentrifuge Anomhanya Zvikuru

Ma centrifuge anomhanyisa akagadzirwa kuti asvike uye arambe achimhanya zvakanyanya, zvichiita kuti nguva dzekuparadzanisa dzikurumidze. Michina iyi inoshandiswa pamabasa akasiyana-siyana, kusanganisira kutsvaga mavhairasi, kupatsanurwa kwemasero, uye kuchenesa nanoparticles. Ma centrifuge anomhanyisa anowedzera kushanda zvakanaka uye kubudisa sampuro, zvichiita kuti dzive dzinokosha mumarabhoritari ane huwandu hwakawanda.

III. Mashandisirwo Ekurapa Nemaindasitiri

3.1. Maindasitiri Ekurapa Nekuongorora

Mukurapa, michina ye centrifuge inokosha pakuongorora nekuongorora zvirwere zvakasiyana-siyana. Inowanzoshandiswa mumakiriniki ekuongororwa kweropa, kusanganisira kuverengwa kweropa rose, kuongororwa kweglucose, uye kupatsanurwa kweropa. Pamusoro pezvo, centrifugation inokosha pakuongorora mvura yemuviri, yakadai seweti nemvura yemusana, zvichibatsira pakuongorora nekutarisa chirwere.

3.2. Kugadzirwa kweMishonga neMishonga

Michina ye centrifuge inoita basa guru muindasitiri yemishonga, ichibatsira mukuwana mishonga, kugadzira, uye kudzora mhando. Inoshandiswa pakuita zvinhu zvakaita sekuparadzanisa zvinhu zvinoshanda zvemishonga (APIs), kucheneswa kwemakemikari, uye kuyedzwa kwemishonga. Centrifuge inobatsira masayendisiti kuwana kupatsanurwa kwakanyatsojeka, zvichibatsira mukugadzirwa kwemishonga zvinobudirira uye kuwedzera kushanda kwayo.

3.3. Sainzi Yezvakatipoteredza

Michina ye centrifuge inoshandiswa zvakanyanya musainzi yezvakatipoteredza uye kutsvagisa, kunyanya mukurapa nekuongorora mvura yakasviba. Nekuparadzanisa zvinhu zvakaoma zvakamiswa kubva mumvura yakasviba, centrifuge inobatsira mukubvisa tsvina, zvichiita kuti mvura ive yakachena. Pamusoro pezvo, inobatsira mukuongorora mienzaniso yevhu, kudzidza maitiro e sedimentation, uye kutarisa kusvibiswa kwezvakatipoteredza.

IV. Mabhenefiti eMichina yeCentrifuge

4.1. Kushanda Nenguva Zvakanaka

Michina yeCentrifuge inoita kuti pave nekukasira kupatsanurwa, zvichideredza zvakanyanya nguva yekugadzirisa kana tichienzanisa nenzira dzinoshandiswa nemaoko. Kushanda zvakanaka kwenguva uku kwakakosha mumashandisirwo anonyanya kukoshesa nguva kana marabhoritari ane simba guru, zvichiita kuti kuongororwa nekutsvagisa kukurumidze.

4.2. Kururama Kwakawedzerwa

Kugadzira centrifugation kunopa mhedzisiro chaiyo, zvichiita kuti zvikamu zvakasiyana zviparadzaniswe zvakanaka. Musiyano wakajeka pakati pemvura nezvinhu zvakaoma zvinowanikwa kuburikidza ne centrifugation unowedzera kuvimbika kwekuyedza kwerabhoritari uye bvunzo dzekuongorora.

4.3. Kuchinja-chinja kwekushandiswa

Michina yeCentrifuge zvishandiso zvinokwanisika kushandiswa mumhando dzakasiyana-siyana dzesamples uye mavhoriyamu. Nemhando dzakasiyana-siyana dzerotors nemaadapters, dzinogona kuchinjika kuzvinodiwa zvakasiyana-siyana zvekutsvagisa, zvichiita kuti dzive dzakakosha mumarabhoritari ane hunyanzvi hwakasiyana-siyana.

4.4. Muenzaniso wekuchengetedza

Maitiro ekudzora centrifugation anobatsira kuchengetedza kusimba kwemasampuli anonzwa. Nekunyatsopatsanura zvikamu zvichienderana nehuwandu, masampuli anochengetedza hunhu hwawo hwepakutanga uye akakodzera kuongororwa kana kuchengetwa.

Mhedziso

Michina yeCentrifuge zvishandiso zvakakosha zvemurabhoritari zvinoshandiswa mumaindasitiri akasiyana-siyana, zvichipa kupatsanurwa kwakavimbika uye kuongororwa kwezvinhu zvakasiyana-siyana. Kunzwisisa mhando dzakasiyana dzemichina yeCentrifuge iripo, mashandisirwo ayo, uye mabhenefiti anoenderana nayo kunobvumira vaongorori nenyanzvi kugadzirisa mashandisirwo ayo, zvichiwedzera kugona kwayo uye mhedzisiro yekutsvagisa.