Ho arola likarolo tsa mali ka sekontiri ho arola likarolo tsa mali joang

Selelekela sa Centrifugation le Bohlokoa ba Eona ho Karohanong ea Mali

Ho Utloisisa Likarolo tsa Mali le Mesebetsi ea Tsona

Mokhoa oa Centrifugation: Tlhaloso ea Mohato ka Mohato

Litšebeliso tsa Centrifugation Libakeng tsa Bongaka le Lipatlisiso

Liphephetso le Nts'etsopele ea Nakong e Tlang ea Karohano ea Likarolo tsa Mali

Selelekela sa Centrifugation le Bohlokoa ba Eona ho Karohanong ea Mali

Ho sebelisa centrifugation ke mokhoa o matla o sebelisoang mafapheng a fapaneng a saense le a bongaka ho arola likarolo tsa metsoako e rarahaneng. E 'ngoe ea ts'ebeliso ea eona ea bohlokoa e ho aroleng likarolo tsa mali, e leng se nang le litlamorao tse kholo tlhahlobong le kalafong ea mafu a fapaneng. Ka ho sebelisa matla a centrifugal, ts'ebetso ena e nolofalletsa ho aroloa ha likarolo tse fapaneng tsa sele le limolek'hule tse teng maling, e leng se lumellang tlhahlobo le lipatlisiso tse ling.

Ho Utloisisa Likarolo tsa Mali le Mesebetsi ea Tsona

Mali, ao hangata a bitsoang "noka ea bophelo," ke mokelikeli o rarahaneng o nang le likarolo tse fapaneng tse etsang mesebetsi ea bohlokoa 'meleng. Likarolo tsena li ka aroloa ka mekhahlelo e meraro e meholo: lisele tse khubelu tsa mali (erythrocyte), lisele tse tšoeu tsa mali (leukocyte), le li-platelet (thrombocyte). Ho phaella moo, plasma, karolo ea mokelikeli ea mali, e na le liprotheine tse fapaneng, lihormone, li-electrolyte le limolek'hule tse ling tsa bohlokoa.

Lisele tse khubelu tsa mali (RBCs) li ikarabella bakeng sa ho jara oksijene liseleng tsa 'mele le ho tlosa carbon dioxide. Lisele tse tšoeu tsa mali (WBCs) li phetha karolo ea bohlokoa tsamaisong ea 'mele ea ho itšireletsa mafung, li sireletsa 'mele khahlanong le tšoaetso le bahlaseli ba tsoang linaheng tse ling. Ka lehlakoreng le leng, li-platelet li bohlokoa bakeng sa ho hoama ha mali, li thibela ho tsoa mali ho feteletseng. Ho utloisisa mesebetsi le litšobotsi tsa likarolo tsena tsa mali ho bohlokoa bakeng sa merero ea tlhahlobo le ea kalafo.

Mokhoa oa Centrifugation: Tlhaloso ea Mohato ka Mohato

Ho arola metsoako ho kenyeletsa karohano ya dikarolo tse fapaneng tsa motswako ho latela boholo ba tsona, bongata le sebopeho. Moelelong wa karohano ya dikarolo tsa madi, tshebetso e qala ka ho bokella disampole tsa madi ho batho ka bomong. Disampole tsena hangata di thibelwa ho etsa madi ho thibela ho hoama ha madi.

Mohato oa 1: Litokisetso le Liphaephe tsa ho Futhumatsa

Disampole tsa madi di bokellwa ka hara diphaephe tse ikgethang tse bitswang diphaephe tsa centrifuge, tse reretsweng ho mamella matla a maholo a kgoheli nakong ya ho centrifuga. Diphaephe di ngotswe ka hloko ho netefatsa hore dikarolo tse arohaneng di tsebahatswa ka nepo.

Mohato oa 2: Ho Seta Centrifuge

Sentifuge e hlomilwe ka di-rotor le di-tube holder tse loketseng, ho nahanelwa mofuta le bophahamo ba disampole tsa madi. Sentifuge e sebetsa ka ho potoloha disampole ka lebelo le phahameng, ka hona e hlahisa matla a sentifuge.

Mohato oa 3: Likarolo tsa Mali tse nang le Mealo

Disampole tsa madi di kenngwa ka hara diphaephe tsa centrifuge, mme ha di potoloha, matla a sebediswang a baka karohano ya dikarolo tsa madi ka dikarolo tse fapaneng. Dikarolo tse teteaneng di dula tlase ho phaephe, ha dikarolo tse se nang boima bo bokaalo di sala ho ya hodimo.

Mohato oa 4: Pokello ea Likarolo

Kamora ho centrifugation, tube e ntšoa ka hloko ho centrifuge, ho netefatsa hore ha ho na ho sisinyeha kapa ho kopanya ho etsahalang. Mealo e aroloa ka hloko ho sebelisoa li-pipettes, lisebelisoa tsa ho hula, kapa lisebelisoa tse khethehileng, ho latela karohano e lakatsehang ea karolo.

Litšebeliso tsa Centrifugation Libakeng tsa Bongaka le Lipatlisiso

Karohano ea likarolo tsa mali ka ho centrifugation e na le mefuta e mengata ea ts'ebeliso maemong a bongaka le a lipatlisiso. Litlhahlobong tsa bongaka, e bapala karolo ea bohlokoa ho khetholleng le ho lekola mafu a kang khaello ea mali, tšoaetso, leukemia le mathata a autoimmune. Ka ho arola likarolo tse itseng, litsebi tsa tlhokomelo ea bophelo li ka etsa tlhahlobo le tlhahlobo e eketsehileng, li thusa ho fumana lefu le nepahetseng le ho lekola kalafo.

Phuputsong, ho centrifugation ho sebelisoa haholo ho batlisisa mafu a amanang le mali, ho ithuta palo e itseng ea lisele, le ho sekaseka matšoao a fapaneng a protheine kapa lintho tsa lefutso tse amanang le maemo a fapaneng. Ka ho arola likarolo tsa mali, bafuputsi ba ka fumana temohisiso ea bohlokoa mabapi le mekhoa ea mafu, ba nts'etsapele mekhoa e mecha ea phekolo, le ho lekola katleho ea kalafo.

Liphephetso le Nts'etsopele ea Nakong e Tlang ea Karohano ea Likarolo tsa Mali

Leha ho centrifugation ho ipakile e le mokhoa oa bohlokoa bakeng sa karohano ea likarolo tsa mali, ho tla le liphephetso tse itseng. Bothata bo bong bo boholo ke monyetla oa ho silafatsoa kapa ho arohana ho sa fellang ka lebaka la ho kopana ha likarolo tse ling. Ho rarolla bothata bona ho hloka ntlafatso e hlokolosi ea liparamente tsa centrifugation, ho kenyeletsoa lebelo, nako le mocheso.

Ho feta moo, mekhoa ea hona joale ea karohano ea mali hangata e hloka lisebelisoa tse kholo le tse turang tsa centrifuge, e leng se fokotsang phihlello ea tsona libakeng tse fokolang tsa mehloli. Leha ho le joalo, lipatlisiso tse tsoelang pele li shebane le ho nts'etsapele mahlale a nkehang habobebe, a theko e tlase a centrifuge ho etsa hore karohano ea likarolo tsa mali e fumanehe habonolo lefatšeng ka bophara.

Ha re sheba pele, tsoelo-pele e kang lisebelisoa tsa microfluidic, mahlale a laboratori le mekhoa e mecha ea karohano e ka fetola karohano ea likarolo tsa mali. Mahlale ana a macha a ka ntlafatsa bokhoni, lebelo le ts'epo ea karohano ea likarolo tsa mali, a bula tsela bakeng sa tlhahlobo e ntlafetseng, meriana e ikhethileng, le mekhoa ea phekolo e shebaneng haholoanyane.

Qetello

Qetellong, ho centrifugation ho bapala karolo ea bohlokoa karohanong ea likarolo tsa mali, ho thusa tlhahlobong ea bongaka, lipatlisisong le nts'etsopele ea kalafo. Ka ho utloisisa melao-motheo e ka morao ho mokhoa ona le ho ntlafatsa ka hloko likarolo tsa ts'ebetso, litsebi tsa tlhokomelo ea bophelo le bafuputsi ba ka arola le ho sekaseka likarolo ka bomong tsa mali, ka hona ba fumana temohisiso ea bohlokoa ka mafu a fapaneng le ho ntlafatsa tlhokomelo ea bakuli.