Sentrifuge e arola thepa joang

Sesepa sa Centrifuge se Arohanya Lisebelisoa Joang?

Selelekela:

Li-centrifuge ke lisebelisoa tse matla le tsa bohlokoa tsa saense tse sebelisoang mafapheng a fapaneng, joalo ka lilaboratori tsa bongaka, lits'ebeletso tsa lipatlisiso le lits'ebetso tsa indasteri. Li bapala karolo ea bohlokoa ho aroleng thepa e fapaneng ho latela boholo ba tsona, bongata ba tsona le thepa e meng ea 'mele. Sehloohong sena, re tla teba lefatšeng le khahlang la ho centrifuge le ho hlahloba mekhoa e fapaneng eo ka eona li-centrifuge li arolang thepa ka katleho.

I. Molao-motheo oa Centrifugation:

Ho centrifugation ho itshetlehile hodima molao-motheo wa ho sedimentation, o sebedisang monyetla wa dipharologanyo tsa bongata pakeng tsa dikarolwana. Ha motswako o kenngwa ka hara centrifuge mme o ohla ka lebelo le phahameng, matla a centrifugal a etsa hore dikarolwana tse teteaneng di fallele ka nqane, ha dikarolwana tse bobebe di atisa ho dula bohareng. Karohano ena e dumella tlhahlobo e eketsehileng le ho arola dikarolwana tse lakatsehang.

II. Likarolo tsa Centrifuge:

1. Kopano ea Rotor:

Karolo ea bohlokoa ea centrifuge efe kapa efe ke kopano ea rotor, e ikarabellang bakeng sa ho tšoara lisampole le ho li beha lebelong le phahameng la ho potoloha. Lirotor li tla ka meralo e fapaneng, joalo ka li-angle tse tsitsitseng, bakete e sothang, le li-rotor tse otlolohileng, e 'ngoe le e 'ngoe e loketse litlhoko tse fapaneng tsa karohano.

2. Liphaephe tsa Centrifuge:

Liphaephe tsa centrifuge, tseo hangata li entsoeng ka thepa ea boleng bo holimo joalo ka polypropylene kapa khalase, li tšoara lisampole nakong ea centrifuge. Liphaephe tsena li khethoa ka hloko ho latela hore na li lumellana joang le lebelo, mocheso le mokhoa oa karohano o lakatsehang.

III. Phapang ea ho Fokotsa Mocheso:

Ho arola dipharologanyo tsa dipharologanyo ke mokhoa o sebediswang haholo ho arola disebediswa tsa boholo bo fapaneng. Molao-motheo o ka mora mokgwa ona o itshetlehile ho fetoleng nako le lebelo la tshebetso ya ho arola dipharologanyo. Ka ho sebedisa letoto la mehato e latellanang ya ho arola dipharologanyo ka lebelo le fapaneng, dikarolwana di ka arolwa ka dikarolwana tse fapaneng ho latela sekgahla sa tsona sa ho arola dipharologanyo. Dikarolwana tse boima di dula kapele mme di ka arolwa ho tse bobebe ka ho latellana.

IV. Ho Fokotsa Botenya ba Gradient:

1. Molao-motheo:

Ho centrifugation ea gradient e teteaneng ke mokhoa o matla o sebelisang liphapang tsa bongata ba likaroloana ho fihlela karohano. Ka ho sebelisa tharollo e nang le gradient e tsitsitseng ea bongata, likaroloana tsa bongata bo fapaneng li ka beoa maemong a fapaneng ka har'a tube nakong ea ho centrifugation.

2. Ho Fokotsa ho Sebetsana ha Isopycnic:

Ho isopycnic centrifugation, e tsejoang hape e le equilibrium sedimentation, matla a centrifugal a lokisoa ho leka-lekanya matla a buoyant a sebetsang holim'a likaroloana. Ka lebaka leo, likaroloana li fihla maemong a itseng ka har'a gradient moo bongata ba tsona bo lekanang le ba sebaka se potolohileng. Mokhoa ona o lumella karohano ea thepa ho latela bongata ba tsona ba buoyant.

3. Sekhahla sa ho Centrifugation sa Sebaka:

Ho potoloha ha sekgahla le libaka ho itshetlehile hodima dipharologanyo tsa sekgahla sa ho sekama ha disebediswa ho arola thepa. Ho thehwa sekgahla sa bongata, empa ho ena le ho fihlella tekano, dikarolwana di a falla ka hara sekgahla ho fihlela di fihla maemong a tsona a tekano ya ho sekama ha disebediswa. Mokgwa ona o nolofalletsa karohano ya thepa ho latela bongata le boholo.

V. Ditshebediso tsa Centrifugation:

1. Lilaboratori tsa Bongaka le tsa Bongaka:

Li-centrifuge li sebelisoa haholo lilaboratoring tsa bongaka le tsa bongaka bakeng sa merero ea ho hlahloba. Li thusa ho arola likarolo tsa mali, tse kang lisele tse khubelu tsa mali, lisele tse tšoeu tsa mali le plasma, tse bohlokoa bakeng sa liteko tse fapaneng, ho kenyeletsoa lipalo tse felletseng tsa mali, tlhahlobo ea k'hemistri ea mali le ho lemoha mafu a tšoaetsanoang.

2. Indasteri ea Meriana:

Ho centrifugation ho bapala karolo ea bohlokoa indastering ea meriana, haholo-holo tlhoekisong le karohanong ea metsoako ea phekolo, lisele le limolek'hule tsa biomolecule. E nolofalletsa ho ntšoa ha lintho tse hloekileng 'me e nolofatsa lits'ebetso tse latelang tse kang kristale, ho sefa le chromatography.

3. Biotechnology le Patlisiso ea Liphatsa tsa Lefutso:

Li-centrifuge ke lisebelisoa tsa bohlokoa haholo theknolojing ea baeloji le lipatlisisong tsa liphatsa tsa lefutso. Li sebelisetsoa ho arola DNA, RNA le liprotheine ho tsoa liseleng, hammoho le ho hloekisa liprotheine tse kopantsoeng le ho ithuta li-organelle tsa sele. Ho feta moo, li thusa ho arola le ho sekaseka likarolo tse ka tlas'a sele, tse kang mitochondria le nuclei.

4. Saense ea Tikoloho:

Li-centrifuge li fumana lits'ebetso mahlaleng a tikoloho bakeng sa ho ithuta litšila, tlhahlobo ea seretse, tlhoekiso ea metsi le ho beha leihlo lisampole tse fapaneng tsa baeloji, ho kenyeletsoa le likokoana-hloko, bolele le litšila tse teng metsing, mobung le moeeng.

Qetello:

Di-centrifuge ke disebediswa tse ikgethang tse itshetlehileng hodima melaomotheo ya ho sedimentation le matla a centrifugal ho arola thepa ka katleho. Ebang ke lefapheng la bongaka, indastering ya meriana, kapa dipatlisisong tsa mahlale, centrifuge e thusa ho fumana dikarolo tse hlwekileng, ho arola dintho tsa bohlokwa, le ho sekaseka disampole ka nepo. Ho tloha ho differential centrifuge ho ya ho density gradient centrifuge, disebediswa tsena di fetotse dithuto tse ngata tsa mahlale mme di ntse di le matlotlo a bohlokwa ho ntshetseng pele kutlwisiso ya rona ya lefatshe le re potileng.