Centrifuge inoparadzanisa sei zvinhu

Centrifuge inoparadzanisa sei zvinhu?

Nhanganyaya:

MaCentrifuge zvishandiso zvine simba uye zvakakosha zvesainzi zvinoshandiswa muzvikamu zvakasiyana-siyana, zvakaita semarabhoritari ekurapa, nzvimbo dzekutsvagisa, uye maitiro emaindasitiri. Anoita basa rakakosha mukupatsanura zvinhu zvakasiyana zvichienderana nehukuru hwazvo, huwandu hwazvo, uye zvimwe zvinhu zvemuviri. Muchinyorwa chino, tichaongorora nyika inonakidza yecentrifuge uye tichaongorora nzira dzakasiyana-siyana dzinoshandiswa necentrifuge kupatsanura zvinhu zvinobudirira.

I. Nheyo yeCentrifugation:

Kupinza zvinhu mu centrifugation kwakavakirwa panheyo yekudonha kwemvura, iyo inoshandisa mukana wekusiyana kwehuwandu hwezvinhu zviri pakati pezvinhu. Kana musanganiswa ukaiswa mu centrifuge wotenderedzwa nekumhanya kwakanyanya, simba re centrifugal rinoita kuti zvinhu zviri mu centrifuge zvifambe zvichienda kunze, nepo zvinhu zviri mu centrifugation zvichigara pakati. Kupatsanurwa uku kunobvumira kuongororwa kwakawanda uye kuparadzaniswa kwezvinhu zvinodiwa.

II. Zvikamu zveCentrifuge:

1. Kuungana kweRotor:

Chinhu chakakosha che centrifuge chero ipi zvayo i rotor assembly, iyo ine basa rekubata masampuli uye kuaisa kumhanya kwakanyanya. Ma rotor anouya mumhando dzakasiyana-siyana, dzakadai se fixed-angle, swinging bucket, uye vertical rotors, imwe neimwe yakakodzera zvinodiwa zvakasiyana zvekuparadzanisa.

2. Machubhu eCentrifuge:

Machubhu eCentrifuge, anowanzogadzirwa nezvinhu zvemhando yepamusoro zvakaita sepolypropylene kana girazi, anobata sampuli panguva yecentrifuge. Machubhu aya anosarudzwa nokungwarira zvichienderana nekuenderana kwawo nekumhanya, tembiricha, uye nzira yekuparadzanisa inodiwa.

III. Kusiyanisa kweCentrifugation:

Kuparadzanisa masero esimbi (differential centrifugation) inzira inoshandiswa zvakanyanya kupatsanura zvinhu zvehukuru hwakasiyana. Chinangwa cheiyi nzira ndechekusiyanisa nguva nekumhanya kwemaitiro e centrifugation. Nekushandisa matanho akatevedzana e centrifugation steps nekumhanya kwakasiyana, zvidimbu zvinogona kupatsanurwa kuita zvidimbu zvakasiyana zvichienderana nehuwandu hwazvo hwe sedimentation. Zvikamu zvinorema zvinogara nekukurumidza uye zvinogona kupatsanurwa kubva kune zvakareruka mumatanho anotevera.

IV. Kudzora Kuwanda Kwemagiraidhi:

1. Musimboti:

Kuyera density gradient centrifugation inzira ine simba inoshandisa musiyano wehuwandu hwezvimedu kuti iparadzanise. Nekushandisa mhinduro ine continuous density gradient, zvimedu zve densities zvakasiyana-siyana zvinogona kuiswa panzvimbo dzakasiyana mukati mechubhu panguva yekuchenesa.

2. Kudzora masero eropa neIsopycnic:

Mukushandiswa kwe isopycnic centrifugation, inozivikanwawo se equilibrium sedimentation, simba re centrifugal rinogadziriswa kuti rienzane nesimba re buoyant rinoshanda pazvikamu. Nekuda kweizvozvo, zvikamu zvinosvika panzvimbo dzakatarwa mu gradient apo density yazvo yakaenzana neyenzvimbo yakapoteredza. Iyi nzira inobvumira kupatsanurwa kwezvinhu zvichibva pakuwanda kwazvo kwe buoyant.

3. Kudzora Kushandiswa Kwenzvimbo:

Kuyerera kwenzvimbo pakati pemvura nemhepo kunoenderana nekusiyana kwehuwandu hwemvura nemhepo kubva muzvinhu zvakaparadzana. Kuyera kwehuwandu hwemvura kunowanikwa, asi pachinzvimbo chekusvika pakuenzana, zvidimbu zvinofamba nepakati pemvura kusvika zvasvika panzvimbo dzazvo dzekuenzanisa kwemvura nemhepo. Iyi nzira inobvumira kupatsanurwa kwezvinhu zvichibva pakuenzana uye saizi.

V. Mashandisirwo eCentrifugation:

1. Marabhoritari ekurapa nekiriniki:

Ma "centrifuges" anoshandiswa zvakanyanya mumarabhoritari ekurapa neekiriniki pakuongorora zvirwere. Anobatsira mukupatsanura zvikamu zveropa, zvakaita semasero matsvuku eropa, masero machena eropa, uye plasma, izvo zvakakosha pakuongororwa kwakasiyana-siyana, kusanganisira kuverengwa kweropa rose, kuongororwa kwemakemikari eropa, uye kuonekwa kwezvirwere zvinotapukira.

2. Indasitiri yeMishonga:

Kugadzira centrifugation kunoita basa rakakosha muindasitiri yemishonga, kunyanya mukuchenesa nekuparadzanisa mishonga, masero, uye biomolecules. Kunogonesa kuburitswa kwezvinhu zvakachena uye kunorerutsa maitiro ekutevera akadai sekristaro, kusefa, uye chromatography.

3. Biotechnology uye Kutsvagurudza nezveMajini:

MaCentrifuge zvishandiso zvinokosha mu biotechnology uye tsvakiridzo yemajini. Anoshandiswa pakuparadzanisa DNA, RNA, nemapuroteni kubva mumasero, pamwe nekuchenesa mapuroteni akasanganiswa uye kudzidza ma organelle emasero. Uyezve, anorerutsa kupatsanurwa uye kuongororwa kwezvikamu zve subcellular, zvakaita se mitochondria ne nuclei.

4. Sainzi Yezvakatipoteredza:

Ma "centrifuges" anowana mashandisirwo muzvidzidzo zvezvakatipoteredza pakudzidza zvinosvibisa, kuongorora marara, kuchenesa mvura, uye kutarisa mienzaniso yakasiyana-siyana yezvehupenyu, kusanganisira tupukanana, algae, uye zvinosvibisa zviri mumvura, ivhu, nemhepo.

Mhedziso:

MaCentrifuge zvishandiso zvinoshamisa zvinovimba nemisimboti yekudonha kwevhu uye simba repakati kuti zviparadzanise zvinhu zvinobudirira. Ingava iri mune zvehutano, indasitiri yemishonga, kana tsvakurudzo yesainzi, centrifuge inobatsira mukuwana zvikamu zvakachena, kuparadzanisa zvinhu zvinokosha, uye kuongorora mienzaniso nemazvo. Kubva pakuparadzanisa centrifuge kusvika pakuparadzanisa density gradient, zvishandiso izvi zvakachinja masayendisiti akawanda uye zvichiri kukosha mukusimudzira kunzwisisa kwedu nyika yakatipoteredza.