centrifuge e sebelisoa ho arola DNA ho

Centrifuge: Sesebelisoa sa Phetoho bakeng sa Karohano ea DNA

Selelekela

Phuputsong ea kajeno ea baeloji le ea bongaka, ho ntšoa ha DNA ho phetha karolo ea bohlokoa. Bo-rasaense ba hloka DNA e hloekileng le e sa senyehang ho ithuta litšobotsi tsa liphatsa tsa lefutso, ho hlahisa meriana ea phekolo le ho utloisisa mafu a fapaneng. Sesebelisoa se seng sa bohlokoa ts'ebetsong ena ke centrifuge. Sesebelisoa sena se sebelisa matla a centrifuge ho arola DNA ho likarolo tse ling tsa sele, ho nolofalletsa bafuputsi ho fumana thepa e hloekileng ea liphatsa tsa lefutso. Sehloohong sena, re tla hlahloba lefatše le khahlang la centrifuge le ho hlahloba hore na li kenya letsoho joang ho ntšetseng pele lipatlisiso tsa DNA.

Ho utloisisa Centrifugation

Ho Centrifugation ke mokhoa o sebediswang haholo baelojing le baelojing ho arola metswako ho itshetlehile ka dipharologanyo tsa bongata ba dikarolwana. Centrifuge e fihlella karohano ka ho sebedisa matla a maholo a kgoheli motswakong, e leng se etsang hore dikarolwana tse teteaneng di be le lerole kapa di phaphamale. Tshebetso ena e tshwana le motjeko wa sefefo moo matla a sebetsang hodima motswako, a kganna dikarolwana tsa bongata bo fapaneng ho ya maemong a fapaneng.

Ho Sebelisa Centrifugation bakeng sa Karohano ea DNA

DNA, ka likhoele tsa eona tse telele tse lohellaneng, e hloka ho tšoaroa ka hloko nakong ea ho itšehla thajana. Ho centrifugation ho fana ka tikoloho e laoloang bakeng sa ts'ebetso ena ea karohano. Ka letoto la ho potoloha ho nepahetseng, centrifuge e arola limolek'hule tsa DNA ho likarolo tse ling tsa sele tse kang liprotheine, lipids le RNA, e leng se lumellang bafuputsi ho ithuta litšobotsi tsa eona tse ikhethang.

Bohlokoa ba Karohano ea DNA

DNA, moralo oa bophelo, e na le senotlolo sa tsoelo-pele e se nang palo ea saense. Ebang ke ho hlahloba tsoelo-pele ea boholo-holo ka mesaletsa ea lintho tsa khale kapa ho nts'etsapele meriana e ikhethileng, bohloeki le boleng ba DNA e ntšitsoeng li bohlokoa haholo. Ho sebelisa centrifugation ho phetha karolo ea bohlokoa ho fumaneng sampole ena e hloekileng ea DNA, ka hona ho lumella bafuputsi ho teba liphiring tsa sele.

Mefuta ea Li-Centrifuge

Di-centrifuge di tla ka mefuta e fapaneng, e 'ngoe le e 'ngoe e etselitsoe merero e itseng ea lipatlisiso. Li arotsoe ka lihlopha tse pharaletseng e le li-ultracentrifuge, li-microcentrifuge, le li-preparative centrifuge, lisebelisoa tsena li khotsofatsa litlhoko tse fapaneng tsa lipatlisiso tsa saense.

Li-centrifuge tsa Ultra

Li-ultracentrifuge, joalo ka ha lebitso le bontša, li sebetsa ka lebelo le phahameng haholo, li lumella karohano ea likaroloana tse nyane ho feta micrometer. Kaha li khona ho fihlela matla a matla a khoheli a fetang makhetlo a 500,000, li-ultracentrifuge lia hlokahala ho ithuteng meaho e ka tlas'a sele le ho arola lisampole tse bonolo tsa DNA.

Li-Microcentrifuge

Li-microcentrifuge, ka tekanyo ea tsona e nyane, ke tsona tse sebetsang haholo lilaboratoring tse ngata tsa lipatlisiso. Li-centrifuge tsena li khona ho fihlela matla a khoheli a fihlang makhetlo a 20,000, e leng se etsang hore li be ntle bakeng sa ho ntšoa ha DNA kamehla moo bongata bo bonyenyane ba sampole bo lekaneng.

Li-Centrifuge tsa ho Lokisetsa

E etselitsoe ho etsa bongata bo boholo, li-centrifuge tse lokisetsang lia sebelisoa ha ho hlokahala ho arola bongata bo boholo ba DNA. Mofuta ona oa centrifuge o lumella ho sebetsana le bongata bo boholo ba sampole, e leng se etsang hore e lokele lits'ebetso tsa indasteri tse hlokang ho ntšoa ha DNA ka bongata, joalo ka ho latellana ha DNA kapa tlhahlobo ea forensic.

Mokhoa oa ho Aroloa ha DNA

Ho fumana DNA e hloekileng ka ho centrifugation ho kenyelletsa mehato e 'maloa ea bohlokoa:

1. Tokisetso ea Sampole: Mohato oa pele o kenyelletsa ho aroloa ha thepa ea baeloji e nang le DNA ea thahasello. Ena e ka ba mali, lisele, kapa mohloli ofe kapa ofe oa sele o nang le thepa ea liphatsa tsa lefutso e reriloeng.

2. Lysis ea Lisele: Ho lokolla DNA, lisele lia sitisoa 'me lera la tsona lea robeha, e leng se lumellang hore DNA e lokolloe hore e be tharollo. Ts'ebetso ena e kenyelletsa tšebeliso ea litharollo tse itseng tsa buffer tse sireletsang DNA hore e se ke ea senyeha.

3. Ho Centrifugation: Sampole e lokisitsoeng e beoa ka hara tube ea centrifuge, 'me ka mor'a ho lokisa liparamente tse loketseng, centrifuge e kenngoa tšebetsong. Ho potoloha ho hlahisa matla a matla a ho hula, a etsang hore likaroloana li arohane ho latela bongata ba tsona. Ha nako e ntse e ea, likaroloana tsa DNA tse teteaneng lia theohela tlase ho tube, li siea likarolo tse ling ka holimo.

4. Pokello ea DNA: Hang ha ts'ebetso ea centrifugation e phethehile, mokelikeli o ka holim'a pellet ea DNA o tlosoa ka hloko, ho sala feela mashala a bohlokoa a DNA. Joale DNA e emisoa ka har'a buffer e loketseng, ho netefatsa botsitso ba eona le ho tšoaneleha bakeng sa tlhahlobo e latelang.

Qetello

Bokhoni bo ikhethang ba centrifuge ba ho arola DNA ho likarolo tse ling tsa sele bo fetotse lipatlisiso tsa liphatsa tsa lefutso. Ka centrifugation, bo-rasaense ba fumana monyetla oa ho fumana sampole e hloekileng ea DNA, ea bohlokoa bakeng sa ho utloisisa litšobotsi tsa liphatsa tsa lefutso, ho nts'etsapele mekhoa ea phekolo, le ho ithuta mafu a fapaneng. Ha theknoloji e ntse e tsoela pele, centrifuge e ntse e tsoela pele ho fetoha, e ntlafatsa bokhoni le lebelo la karohano ea DNA. Ka katleho e 'ngoe le e 'ngoe, re atamela haufi le ho notlolla liphiri tse kentsoeng ka har'a likhoele tsa DNA le ho fetola kutloisiso ea rona ea bophelo ka bobona.