Centrifuge inoparadzanisa sei mvura

Nhanganyaya yeCentrifugation neMitemo Yayo

Kuparadzanisa zvinhu nenzira ye "centrifugation" inzira inoshandiswa zvakanyanya yekuparadzanisa zvinhu yakachinja maindasitiri akasiyana-siyana, anosanganisira mishonga, biotechnology, uye kugadzira chikafu. Chinyorwa chino chine chinangwa chekuongorora sainzi iri shure kwe "centrifugation" uye kuti inoparadzanisa sei zvinwiwa zvichibva pakuwanda kwazvo.

Kunzwisisa Nheyo dzeCentrifugation

Pakati payo, centrifugation inotsamira pakushandiswa kwesimba re centrifugal, iro rinogadzirwa nekutenderedza sample kutenderedza central axis. Simba iri rinogadzira nzvimbo yekuparadzanisa iyo inogonesa kunyungudika kwezvikamu kana zvikamu zvichienderana nehuremu hwazvo kana huwandu hwazvo. Zvinhu zvakakora zvinofamba zvichienda kumucheto wekunze, nepo zvinhu zvakareruka kana mvura zvichiramba zviri pedyo nepakati.

Mhando dzeCentrifuges

Mhando dzakasiyana dzema "centrifuge" dziripo, imwe neimwe ichienderana nezvinodiwa zvekuparadzanisa. Kusiyanisa "centrifugation", "ultracentrifugation", uye "density gradient centrifugation" ndedzimwe dzenzira dzinonyanya kushandiswa. Matekiniki aya e "centrifugation" akasiyana maererano nekumhanya, dhizaini ye "rotor", uye mashandisirwo, zvichibvumira kushandiswa kwakasiyana-siyana mukutsvaga kwakasiyana-siyana uye mamiriro emaindasitiri.

Kusiyanisa kweCentrifugation yeKupatsanura

Kuparadzanisa masero (differential centrifugation) inzira inoshandiswa kupatsanura zvidimbu zvehukuru hwakasiyana uye densities. Nekutevera matanho akatevedzana e centrifugation anotangira kubva pakumhanya kwakaderera kusvika pakumhanya kwakakwira zvishoma nezvishoma, zvinokwanisika kuwana zvidimbu zvine maumbirwo akasiyana ezvikamu. Nzira iyi inowanzoshandiswa mu biology yemasero kubvisa nekuparadzanisa zvikamu zvemasero zvakaita se organelles, mapuroteni, uye nucleic acids.

Ultracentrifugation yekuparadzanisa kweHigh-Resolution

Kupinza masero mumasero (Ultracentrifugation) inzira ine simba inoita kuti masayendisiti akwanise kupatsanura zvikamu zvemasero zviri pasi pemasero nemazvo. Nzira iyi inoshandisa kumhanya kwakanyanya uye inowanzoda michina yakasarudzika inozivikanwa se ultracentrifuges. Midziyo yakadaro inogona kutenderedza masampuro nekumhanya kunopfuura 100,000 revolutions paminiti, zvichigadzira masimba makuru e centrifugal.

Density Gradient Centrifugation yeKuparadzanisa Zvinobudirira

Kushandiswa kwe density gradient centrifugation kunoshandisa mutsauko we density yezvinhu zviri mu sample kuti zvibudirire pakupatsanurwa. Nzira iyi inoshandisa kugadzira artificial density gradient, kazhinji uchishandisa mhinduro ine gradient dzakasiyana dzemunyu, shuga, kana mamwe makemikari. Kana ikashandiswa centrifugation, sample inopatsanurwa zvichienderana nemusiyano we density, ichigadzira maband kana zones akasiyana.

Kuongororwa kweCentrifugation yeKuratidza Hunhu

Kunze kwekupatsanurwa, centrifugation inoshandiswawo pakuongorora. Analytical centrifugation inoyera shanduko muhuwandu hwe sedimentation yemamorekuru kana zvidimbu, ichipa ruzivo rwakakosha nezvehukuru hwawo, chimiro, uye huremu hwemamorekuru. Iyi nzira, inozivikanwa se sedimentation velocity kana equilibrium centrifugation, inoita basa rakakosha muminda yakaita se biophysics, protein chemistry, uye polymers.

Mashandisirwo eCentrifugation muMaindasitiri

Kugadzira zvinhu zvinoshandisa centrifuge kwave kushandiswa zvakanyanya mumaindasitiri akasiyana-siyana nekuda kwehunhu hwayo hwekushandisa pakugadzira mishonga. Muchikamu chemishonga, macentrifuge akakosha pakuparadzanisa nekuchenesa mishonga panguva yekugadzira. Maindasitiri ekugadzira chikafu anoshandisa centrifuge kuparadzanisa zvinhu zvakasimba kubva mumvura, kuchenesesa zvinwiwa, uye kubvisa mafuta kubva mumbeu. Pamusoro pezvo, centrifuge inoshandiswa mumafekitori ekuchenesa mvura yakasviba kuparadzanisa zvinhu zvakaoma nezvinwiwa zvinobudirira.

Kufambira Mberi muTekinoroji yeCentrifugation

Mumakore apfuura, tekinoroji ye centrifugation yakaona kufambira mberi kukuru. Ma centrifuge emazuva ano haangogumiri pakumhanya kwakanyanya kutenderera chete asiwo ane zvinhu zvakaita se automated sample handling, control tembiricha, uye real-time monitoring. Kufambira mberi uku kunoita kuti vaongorori nevashandi vemaindasitiri vashande zvakanaka uye vakwanise kudzokorodza maitiro avo ekuparadzanisa.

Mhedziso

Kugadzira centrifugation kwakachinja maindasitiri akasiyana-siyana nekupa nzira yakasimba uye inoshanda-chinja yekuparadzanisa zvinwiwa zvichienderana nehuwandu hwazvo. Ingave kuparadzanisa zvikamu zvemasero kana kuchenesa zvigadzirwa zvemishonga, centrifugation inoita basa rakakosha mukusimudzira kutsvagisa uye maitiro emaindasitiri. Nekufambira mberi kwetekinoroji kuri kuenderera mberi, ramangwana rekugadzira centrifugation rinovimbisa kunyatsojeka uye kushanda zvakanaka, zvichichinja minda yakawanda nemashandisirwo.