chifukwa chiyani magazi amagawanika mu centrifuge

N’chifukwa Chiyani Magazi Amagawanika mu Centrifuge?

Kumvetsetsa Zigawo za Magazi

Udindo wa Centrifugation mu Kulekanitsa Magazi

Zinthu Zomwe Zimakhudza Kulekanitsa Magazi mu Centrifuge

Njira Zosiyanasiyana Zopatulira Magazi

Kugwiritsa Ntchito Njira Zolekanitsira Magazi

Kulekanitsa magazi pogwiritsa ntchito centrifuge ndi njira yofala m'ma laboratories azachipatala ndi m'malo ofufuza. Njira imeneyi imalola asayansi kupatula ndikuphunzira zigawo zinazake za magazi, kuphatikizapo maselo ofiira a magazi, maselo oyera a magazi, ma platelet, ndi plasma. Kumvetsetsa chifukwa chake magazi amalekanitsidwa mu centrifuge kumafuna kudziwa zigawo zosiyanasiyana ndi mawonekedwe awo enieni.

Kumvetsetsa Zigawo za Magazi

Magazi ndi madzi ofunikira kwambiri m'thupi omwe amagwira ntchito zofunika kwambiri, monga kunyamula mpweya, chitetezo cha mthupi, ndi kutseka magazi. Amapangidwa ndi zigawo zosiyanasiyana, chilichonse chili ndi makhalidwe ake ndi ntchito zake. Zigawozi zikuphatikizapo maselo ofiira a magazi, maselo oyera a magazi, ma platelet, ndi plasma.

Maselo ofiira a m'magazi, kapena ma erythrocyte, ndi omwe amanyamula mpweya m'thupi lonse. Ali ndi puloteni yotchedwa hemoglobin, yomwe imagwirizana ndi mamolekyu a mpweya, zomwe zimathandiza kuti mpweya ufike bwino. Maselo oyera a m'magazi, kapena ma leukocyte, amachita gawo lofunika kwambiri pa chitetezo chamthupi, kuteteza thupi ku matenda ndi matenda. Ma platelet, kapena ma thrombocyte, amagwira ntchito yoteteza magazi kuundana, kuteteza kutuluka magazi kwambiri. Plasma ndi gawo lamadzimadzi m'magazi lomwe limanyamula zakudya, mahomoni, ndi zinyalala.

Udindo wa Centrifugation mu Kulekanitsa Magazi

Kugawa magazi m'magazi ndi njira yomwe imagwiritsa ntchito mphamvu ya centrifugal kulekanitsa zigawo za chisakanizo kutengera kuchuluka kwawo. Pankhani ya magazi, kugawa magazi m'magazi kumalola kulekanitsa zigawo zosiyanasiyana za magazi.

Kachipangizo kozungulira ndi chipangizo chomwe chimazungulira zitsanzo pa liwiro lalikulu, ndikupanga mphamvu yozungulira. Magazi akayikidwa mu kachipangizo kozungulira, kuyenda kozungulira kumapangitsa kuti zigawozo zilekanitsidwe kutengera kuchuluka kwawo. Zigawo zolemera, monga maselo ofiira a magazi ndi ma platelet, zimapita pansi pa chubu chozungulira, pomwe zigawo zopepuka, monga plasma, zimakwera pamwamba.

Zinthu Zomwe Zimakhudza Kulekanitsa Magazi mu Centrifuge

Zinthu zingapo zingakhudze momwe magazi amagawidwira bwino komanso momwe magazi amagawidwira bwino mu centrifuge. Zinthuzi zikuphatikizapo liwiro la kuthamanga kwa magazi, nthawi ya centrifuge, ndi kutentha.

Liwiro la kuthamanga, kapena liwiro limene centrifuge imazungulira, ndi chinthu chofunikira kwambiri pakulekanitsa magazi. Kuthamanga kosakwanira kwa kuthamanga sikungapangitse mphamvu yokwanira kulekanitsa zigawozo mokwanira, zomwe zimapangitsa kuti pakhale kusiyana kosakwanira. Mosiyana ndi zimenezi, kuthamanga kwambiri kwa kuthamanga kungayambitse kuwonongeka kwa zitsanzo kapena kusakanikirana kosafunikira kwa zigawozo.

Kutalika kwa nthawi yogawa magazi m'magazi kumatsimikizira kuchuluka kwa kulekanitsidwa kwa magazi. Kuzungulira kwa nthawi yayitali kumalola kulekanitsidwa kwakukulu, kuonetsetsa kuti gawo lililonse lagawidwa mokwanira. Komabe, kugawa magazi m'magazi m'magazi m'magazi m'magazi kwa nthawi yayitali kungayambitse maselo ofiira a magazi kusweka kapena kusweka, zomwe zimakhudza kulondola kwa zotsatira zake.

Kutentha kumachitanso gawo lofunika kwambiri pakulekanitsa magazi. Kuziziritsa centrifuge ndikusunga kutentha kotsika musanayambe komanso panthawi yotseka kumachepetsa chiopsezo cha kuwonongeka kwa zigawo zamagazi. Kuphatikiza apo, zigawo zina, monga plasma, zitha kukhala ndi vuto la kutentha, zomwe zimapangitsa kuti kuwongolera kutentha kukhale kofunika kuti zisungidwe.

Njira Zosiyanasiyana Zopatulira Magazi

Pali njira zosiyanasiyana zopatulira magazi, iliyonse ili ndi ubwino wake komanso ntchito zake. Njira ziwiri zomwe zimagwiritsidwa ntchito kwambiri ndi differential centrifugation ndi density gradient centrifugation.

Kusiyanitsa kwa centrifugation kumaphatikizapo masitepe angapo a centrifugation pang'onopang'ono kukulitsa liwiro. Njirayi imalola kulekanitsa zigawo zazikulu zamagazi, monga maselo ofiira amagazi, ndi zigawo zazing'ono monga maselo oyera amagazi ndi ma platelet. Mwa kusintha magawo a centrifugation, zigawo zinazake zimatha kugawidwa bwino.

Kuyeza kuchuluka kwa magazi pogwiritsa ntchito njira yoyezera kuchuluka kwa magazi (density gradient centrifugation), nthawi zambiri imakhala yankho la sucrose kapena ficoll, kuti ithandize kulekanitsa magazi. Pamene magazi aikidwa pamwamba pa njira yoyezera kuchuluka kwa magazi (density gradient medium) ndi kuikidwa pakati, zigawozo zimadutsa m'njira yoyezera kuchuluka kwa magazi kutengera kuchuluka kwawo. Njira imeneyi imapereka magawo oyeretsedwa kwambiri a zigawo za magazi, zomwe zimapangitsa kuti zikhale zoyenera kufufuza ndi kugwiritsa ntchito pozindikira matenda.

Kugwiritsa Ntchito Njira Zolekanitsira Magazi

Njira zolekanitsira magazi zimagwiritsidwa ntchito kwambiri m'magawo osiyanasiyana azachipatala ndi kafukufuku. Mwachitsanzo, pozindikira matenda, kulekanitsa plasma kumalola kuyeza molondola kwa biochemistry yamagazi, monga kuwunika kuchuluka kwa shuga m'magazi, momwe chiwindi chimagwira ntchito, kapena mawonekedwe a lipid. Kupatula zigawo zinazake zamagazi kumathandiza kusanthula kwina pazinthu zosiyanasiyana zofufuzira, kuphatikizapo kuphunzira matenda, kufufuza zinthu za majini, ndikupanga njira zatsopano zochiritsira.

Kuphatikiza apo, kulekanitsa magazi kumachita gawo lofunika kwambiri pa njira zothira magazi. Kulekanitsa magazi m'zigawo zake kumathandiza kuti magazi azigwiritsidwa ntchito moyenera, kuyezetsa kuti magaziwo akugwirizana ndi magazi, komanso kupanga zinthu zapadera monga ma platelet concentrate ndi mankhwala ochokera ku plasma.

Pomaliza, magazi amalekanitsidwa mu centrifuge chifukwa cha kuchulukana kosiyanasiyana kwa zigawo zake. Kumvetsetsa zigawo zosiyanasiyana za magazi ndi mawonekedwe awo ndikofunikira kuti magazi alekanitsidwe bwino. Zinthu monga liwiro la kuthamanga, nthawi ya centrifuge, ndi kutentha zimakhudza njirayi, pomwe njira monga differential centrifugation ndi density gradient centrifugation zimagwiritsidwa ntchito kwambiri. Kugwiritsa ntchito njira zolekanitsira magazi kumayambira pa matenda mpaka kafukufuku ndi mankhwala oika magazi, zomwe zimapangitsa kuti ikhale njira yofunika kwambiri m'munda wa zamoyo ndi chisamaliro chaumoyo.