Kupanga mwaukadaulo makina olekanitsa ma centrifuge ndi opanga mankhwala - Zonelink
momwe mungalekanitsire magazi popanda centrifuge
Momwe Mungalekanitsire Magazi Popanda Centrifuge
Chiyambi
Kulekanitsa magazi ndi njira yofunika kwambiri yomwe imagwiritsidwa ntchito kwambiri m'ma laboratories ofufuza, m'zipatala, komanso m'malo oyezera matenda. Njira zachikhalidwe zimaphatikizapo kugwiritsa ntchito centrifuge, yomwe ingatenge nthawi, yokwera mtengo, komanso imafuna zida zinazake. Komabe, pali njira zina zomwe zimathandiza kulekanitsa magazi popanda kugwiritsa ntchito centrifuge. M'nkhaniyi, tifufuza njira zisanu zosiyanasiyana zolekanitsira magazi pogwiritsa ntchito zipangizo zosavuta kupeza komanso njira zosavuta. Njirazi zingagwiritsidwe ntchito pazochitika zadzidzidzi, malo ochepa, kapena ngakhale kunyumba ngati pakufunika kutero.
I. Kugwiritsa Ntchito Mphamvu Yokoka ndi Nthawi
Njira yosavuta kwambiri yolekanitsira zigawo za magazi imagwiritsa ntchito mphamvu yokoka ndi nthawi. Mu njira iyi, magazi amasonkhanitsidwa mu chubu chopanda tizilombo kapena chidebe ndipo amaloledwa kukhala osasokonezeka. Pakapita nthawi, zigawo za magazi zimagawikana m'magawo osiyanasiyana kutengera kuchuluka kwawo.
Ndondomeko:
1. Sonkhanitsani zitsanzo za magazi mu chubu chopanda tizilombo toyambitsa matenda kapena chidebe.
2. Onetsetsani kuti chubucho sichikusokonezedwa kwa mphindi zosachepera 30.
3. Pambuyo pa nthawi yoikidwiratu, yang'anani kupangika kwa zigawo zosiyana m'magazi.
4. Thirani mosamala gawo lapamwamba (plasma kapena seramu).
5. Gwiritsani ntchito pipette yoyera kuti musonkhanitse wosanjikiza wolekanitsidwa.
Ubwino:
- Palibe zida zofunika.
- Kufunika kulowererapo pang'ono.
- Yoyenera pazochitika zadzidzidzi kapena makonda omwe alibe zinthu zambiri.
II. Kugwiritsa Ntchito Pepala Losefera
Pepala losefera lingagwiritsidwenso ntchito kulekanitsa zigawo za magazi m'njira yosavuta komanso yotsika mtengo. Njirayi imagwiritsa ntchito mphamvu ya capillary, pomwe madzi amatuluka kudzera m'njira zazing'ono, zopapatiza kapena m'mabowo.
Ndondomeko:
1. Pezani pepala losefera ndikudula m'zidutswa.
2. Ikani dontho laling'ono la magazi pa pepala losefera.
3. Lolani kuti magazi anu aume bwino.
4. Konzani chidebe choyenera (monga beaker) chokhala ndi chosungunulira (monga madzi kapena saline).
5. Ikani mbali imodzi ya pepala losefera m'chidebecho, kuonetsetsa kuti malo otuluka magazi akupitirira muyeso wa madzi.
6. Lolani chosungunulira chikwere kudzera mu mzere wa pepala losefera.
7. Pamene chosungunulira chikuyenda, zigawo zosiyanasiyana za magazi zimapatukana ndikupanga mipiringidzo yosiyana.
8. Dulani mosamala pepala losefera kuti mulekanitse mikanda ya zigawo zomwe mukufuna.
Ubwino:
- Zipangizo zosavuta komanso zotsika mtengo.
- Zimathandiza kulekanitsa zinthu m'njira yosavuta kunyamula komanso yopepuka.
- Yoyenera kugwiritsa ntchito poyenda kapena ntchito za m'munda.
III. Kugwiritsa Ntchito Hemastix
Hemastix, yomwe imagwiritsidwa ntchito kwambiri poyesa mkodzo, ingagwiritsidwenso ntchito polekanitsa magazi. Hemastix ndi timizere tapulasitiki tomwe tili ndi reagent yomwe imagwira ntchito ku magazi, zomwe zimathandiza kulekanitsa zigawo zosiyanasiyana za magazi.
Ndondomeko:
1. Ikani Hemastix moyimirira.
2. Pakani pang'onopang'ono dontho la magazi pamalo ochitira reagent a Hemastix.
3. Lolani kuti magazi ayesedwe ndi reagent kwa nthawi yomwe yatchulidwa.
4. Hemastix idzawonetsa mitundu kapena mapangidwe osiyanasiyana kutalika kwake, kusonyeza kulekanitsidwa kwa maselo a magazi, plasma, ndi zigawo zina.
5. Dulani Hemastix pamalo omwe mukufuna kuti musonkhanitse zigawo zolekanitsidwa.
Ubwino:
- Imapezeka mosavuta m'masitolo ogulitsa zinthu zachipatala.
- Njira yofulumira komanso yosavuta.
- Yoyenera malo oyezera matenda kapena zosowa za kanthawi zolekanitsa magazi.
IV. Kugwiritsa Ntchito Microfluidics
Microfluidics imatanthauza kusintha kwa madzi ang'onoang'ono m'njira zazing'ono kapena ting'onoting'ono. Njira imeneyi yatchuka kwambiri m'zaka zaposachedwa chifukwa cha kugwira ntchito kwake pogawanitsa zigawo za magazi, pakati pa ntchito zina.
Ndondomeko:
1. Pezani chipu cha microfluidic chopangidwira kulekanitsa magazi.
2. Ikani magazi mosamala m'malo osungiramo madzi kapena malo olowera omwe ali pa chip.
3. Ikani mphamvu kapena lolani chitsanzocho chiyende kudzera mu njira zazing'ono.
4. Chip cha microfluidic chidzalekanitsa zigawo za magazi pogwiritsa ntchito kusiyana kwa kukula kwa maselo, mawonekedwe, kapena kufooka kwawo.
5. Sonkhanitsani zigawo zosiyana pa malo otulutsira omwe ali pa chip.
Ubwino:
- Kulekanitsa kolondola m'nthawi yochepa.
- Kuchuluka kochepa kwa chitsanzo kumafunika.
- Yabwino kwambiri pa malo ofufuzira komanso malo ophunzirira matenda apamwamba.
V. Kugwiritsa Ntchito Kulekanitsa Kochokera ku Gel
Kulekanitsa pogwiritsa ntchito gel ndi njira ina yothandiza yomwe imadalira kusamuka kosiyana kwa zigawo za magazi mkati mwa gel matrix. Zigawozo zimasiyana malinga ndi kukula kwawo, kuchuluka kwawo, ndi mphamvu yawo.
Ndondomeko:
1. Pezani chubu cholekanitsa jeli (chomwe chimapezeka m'masitolo ogulitsa zinthu zachipatala).
2. Sonkhanitsani magazi mu chubu ndikusakaniza bwino ndi gel powatembenuza kangapo.
3. Siyani chisakanizocho osasokonezedwa kwa nthawi yomwe yatchulidwa kuti chilole kulekanitsidwa.
4. Pakapita nthawi, zigawozo zidzapatukana, ndikupanga mipiringidzo yosiyana mkati mwa gel matrix.
5. Sakanizani chubucho kwa kanthawi kuti zigawo zolekanitsidwazo zikhazikike m'dera laling'ono.
6. Gwiritsani ntchito njira yoyenera, monga kuyika mapaipi kapena kudula, kuti mulekanitse mipiringidzo yomwe mukufuna kuti musonkhanitse.
Ubwino:
- Zosavuta kwambiri kuposa njira yachikhalidwe yolumikizira magetsi.
- Imapereka mawonekedwe apamwamba komanso kulekanitsa bwino poyerekeza ndi njira zina.
- Yoyenera kugwiritsidwa ntchito m'njira zosiyanasiyana, kuphatikizapo kafukufuku, matenda, kapena mtundu wa magazi.
Mapeto
Kulekanitsa magazi popanda centrifuge n'kotheka pogwiritsa ntchito njira zingapo. Pogwiritsa ntchito mphamvu yokoka ndi nthawi, pepala losefera, Hemastix, microfluidics, kapena njira zolekanitsira zochokera ku gel, zigawo zosiyanasiyana za magazi zimatha kulekanitsidwa ngakhale popanda kugwiritsa ntchito centrifuge. Njirazi zimapereka kusinthasintha, kugwiritsa ntchito ndalama zochepa, komanso kuthekera kolekanitsa magazi m'malo osiyanasiyana. Komabe, ndikofunikira kusintha njira iliyonse kuti igwirizane ndi zofunikira zinazake ndikutsatira njira zotetezeka komanso zoyera panthawi ya ndondomekoyi.
.