me pēhea te wehewehe i te toto me te kore he centrifuge
Me pēhea te wehewehe i te toto me te kore he centrifuge
Kupu Whakataki
He tukanga nui te wehenga toto e whakamahia whānuitia ana i roto i ngā taiwhanga rangahau, i ngā whare hauora, me ngā pokapū tātaritanga. Ko ngā tikanga tuku iho ko te whakamahi i te centrifuge, he mea e pau ana te wā, e utu nui ana, e hiahiatia ana hoki he taputapu motuhake. Heoi anō, kei reira ētahi atu tikanga hei wehe i te toto me te kore e hiahiatia he centrifuge. I roto i tēnei tuhinga, ka tūhuratia e mātou e rima ngā tikanga rerekē hei wehewehe i te toto mā te whakamahi i ngā rauemi e wātea ana me ngā tikanga māmā. Ka taea te whakamahi i ēnei tikanga i roto i ngā āhuatanga ohorere, i ngā taiao iti-rauemi, i te kāinga rānei ina hiahiatia.
I. Te Whakamahi i te Āhuatanga Taumaha me te Wā
Ko te tikanga tino taketake hei wehewehe i ngā wāhanga toto ko te whakamahi i te kaha ā-papa me te wā. I tēnei tikanga, ka kohia te toto ki roto i tētahi ngongo, i tētahi ipu rānei kua horoia, ā, ka waiho kia noho noa iho. I roto i te wā, ka wehea noatia ngā wāhanga toto ki ngā paparanga motuhake i runga i tō rātou matotoru.
Tukanga:
1. Kohia ngā tauira toto ki roto i tētahi ngongo, i tētahi ipu rānei kua horoia.
2. Kia kaua e whakakorikoria te ngongo mō te 30 meneti pea.
3. I muri i te wā kua whakaritea, mātakitakina te hanganga o ngā paparanga motuhake i roto i te toto.
4. Āta tāpirihia, ringihia rānei te paparanga o runga (te plasma, te serum rānei).
5. Whakamahia he pipette parakore hei kohi i te paparanga kua wehea.
Ngā Painga:
- Kāore he taputapu e hiahiatia ana.
- He iti noa te wawaotanga e hiahiatia ana.
- He pai mō ngā āhuatanga ohorere, mō ngā tautuhinga iti-rauemi rānei.
II. Te Whakamahi i te Pepa Tātari
Ka taea hoki te whakamahi i te pepa tātari hei wehewehe i ngā wāhanga toto i roto i tētahi huarahi māmā, whai hua hoki. Ka whakamahia e tēnei tikanga te mahi a te capillary, arā, ko te putanga ake o te wai mā roto i ngā awaawa iti, whaiti, i ngā pūwero rānei.
Tukanga:
1. Tikina he pepa tātari, ka tapatapahia kia rīpene.
2. Pania he pata toto iti ki runga i te rīpene pepa tātari.
3. Tukua kia maroke rawa te tauira toto.
4. Whakaritea he ipu e tika ana (hei tauira, he beaker) me te whakarewa (pēnei i te wai, i te tote rānei).
5. Whakatakotoria tetahi pito o te rīpene pepa tātari ki roto i te ipu, me te whakarite kia noho tonu te ira toto ki runga ake i te taumata wai.
6. Tukua te whakarewa kia piki ake mā roto i te rīpene pepa tātari.
7. I te nekehanga o te whakarewa, ka wehe ngā wāhanga toto rerekē, ka hanga he here motuhake.
8. Tapahia marietia te pepa tātari hei wehewehe i ngā whitiki wāhanga e hiahiatia ana.
Ngā Painga:
- He māmā noa iho ngā rauemi, ā, he iti hoki te utu.
- Ka taea te wehewehe i roto i te huarahi kawe, māmā hoki.
- He pai mō ngā mahi haereere, mō ngā mahi papa rānei.
III. Te Whakamahi i te Hemastix
Ka taea hoki te whakamahi i te Hemastix, e whakamahia whānuitia ana mō te whakamātautau mimi, hei wehewehe i te toto. He rīpene kirihou ngā Hemastix kei roto he matū e tauhohe ana ki te toto, e āhei ai te wehewehe i ngā wāhanga toto rerekē.
Tukanga:
1. Whakatakotoria te Hemastix kia poutū.
2. Pania mārire he pata toto ki te wāhi matū o te Hemastix.
3. Tukua te tauira toto kia tauhohe ki te matū mō te wā kua tohua.
4. Ka whakaatuhia e te Hemastix ngā tae rerekē, ngā tauira rānei puta noa i tōna roanga, e tohu ana i te wehenga o ngā pūtau toto, te parāoa toto, me ētahi atu wāhanga.
5. Tapahia te Hemastix ki ngā wāhi whitiki e hiahiatia ana hei kohikohi i ngā wāhanga kua wehea.
Ngā Painga:
- E wātea ana i ngā toa taputapu hauora.
- He tukanga tere, māmā hoki.
- He pai mō ngā pokapū tātaritanga, mō ngā hiahia wehenga toto rangitahi rānei.
IV. Te Whakamahi i ngā Maikorowai
Ko te microfluidics e pā ana ki te whakahaere i ngā rahinga wai iti i roto i ngā ara iti, i ngā maramara ririki rānei. Kua piki te rongonui o tēnei tikanga i ngā tau tata nei nā tōna whai huatanga ki te wehewehe i ngā wāhanga toto, me ētahi atu whakamahinga.
Tukanga:
1. Tikina he maramara moroiti wai i hangaia hei wehewehe i te toto.
2. Āta utaina te tauira toto ki roto i ngā puna, i ngā tomokanga rānei kua tohua i runga i te maramara.
3. Pēhia, tukua rānei te tauira kia rere mā roto i ngā awa iti.
4. Ka wehea e te maramara moroiti-wai ngā wāhanga toto mā te whakamahi i ngā rerekētanga o te rahi, te āhua, te āhua rerekē rānei o te pūtau.
5. Kohia ngā wāhanga motuhake ki ngā putanga kua tohua i runga i te maramara.
Ngā Painga:
- Te wehenga tika i roto i te wā poto.
- He iti rawa te rahinga tauira e hiahiatia ana.
- He pai mō ngā taiwhanga rangahau me ngā tautuhinga tātaritanga matatau.
V. Te Whakamahi i te Wehenga i runga i te Gel
Ko te wehenga i runga i te tiēre tētahi atu tikanga whai hua e whakawhirinaki ana ki te hekenga rerekē o ngā wāhanga toto i roto i te matrix tiēre. Ka wehea ngā wāhanga i runga i tō rātou rahi, te mātotoru, me te utu.
Tukanga:
1. Tikina he ngongo wehewehe kirikiri (e wātea ana i ngā toa taputapu hauora).
2. Kohia te tauira toto ki roto i te ngongo ka whakaranua kia pai ki te tiēre mā te huripokitia kia maha ngā wā.
3. Waiho te ranunga kia kaua e whakakorikoria mō te wā kua whakaritea kia taea ai te wehewehe.
4. Ka haere te wā, ka wehe ngā wāhanga, ka hanga he whitiki motuhake i roto i te anga tiēre.
5. Whakapokaia te ngongo mō te wā poto kia kukū ngā wāhanga kua wehea ki tētahi wāhi iti ake.
6. Whakamahia he tikanga tika, pērā i te whakamahi i te pipet, te tapahi rānei, hei wehe ā-tinana i ngā whitiki e hiahiatia ana hei kohi.
Ngā Painga:
- He māmā ake i te centrifugation tuku iho.
- He teitei ake te taumira me te wehewehenga pai ake i ērā atu tikanga.
- He pai mō ngā momo mahi, tae atu ki te rangahau, te tātaritanga mate, te whakarōpū toto rānei.
Whakamutunga
Ka taea te wehewehe toto me te kore he centrifuge mā te whakamahi i ētahi atu tikanga. Mā te whakamahi i te kaha ā-papa me te wā, te pepa tātari, te Hemastix, ngā tikanga microfluidics, ngā tikanga wehewehe i runga i te gel rānei, ka taea te wehewehe i ngā wāhanga toto motuhake ahakoa kāore he urunga ki te centrifuge. Ka tukuna e ēnei tikanga he ngāwari, he whai hua te utu, me te kaha ki te mahi wehewehe toto i roto i ngā tautuhinga rerekē. Heoi, he mea nui kia whakarerekētia ia tikanga kia rite ki ngā whakaritenga motuhake me te piri ki ngā tikanga haumaru me te parakore i te wā o te tukanga.
.