indlela yokusika ipuleti lokukhulisa i-centrifuge ngaphandle komshini

Kuyini i-Centrifugation kanye nokubaluleka kwayo emasikweni eseli?

I-Centrifugation iyinqubo ebalulekile ocwaningweni lwesayensi kanye nezinqubo zelebhu, ikakhulukazi emkhakheni wezolimo zamaseli. Isetshenziselwa ukuhlukanisa izingxenye ezahlukene zengxube ngokusekelwe ekubuneni kwazo kanye namazinga okudayiswa kwenhlabathi. Ngayo, abacwaningi bayakwazi ukuhlukanisa nokugxila amaseli, ama-organelle, amaprotheni, nezinye izinhlayiya ezithakazelisayo ukuze kuhlaziywe noma kuhlolwe okwengeziwe.

Kodwa-ke, ezimweni ezithile, ukufinyelela emshinini we-centrifuge kungase kuncishiswe noma kungatholakali. Ezimweni ezinjalo, kuba kubalulekile ukuhlola ezinye izindlela zokufeza imiphumela efana ne-centrifugation kuma-culture plate. Lesi sihloko sihlola amasu ahlukahlukene kanye namasu okuhlanganisa i-centrifuge ku-culture plate ngaphandle komshini, sinikeza amathiphu awusizo kubacwaningi nososayensi ababhekene nezimo ezinjalo.

Ukusebenzisa i-Gravity-Assisted Sedimentation

Ukufakwa kwe-sedimentation okusizwa amandla adonsela phansi kuyindlela elula nephumelelayo engalingisa inqubo ye-centrifugation ngaphandle kwesidingo semishini ekhethekile. Ukuze wenze le ndlela, landela lezi zinyathelo:

1. Lungisa ipuleti lakho lokukhulisa ngokufaka isampula ngokucophelela emthonjeni ngamunye. Qinisekisa ukuthi umthamo woketshezi uyafana kuyo yonke imithombo ukuze uthole imiphumela enembile.

2. Beka ipuleti lokukhulisa endaweni ezinzile, evundlile, uqinisekise ukuthi aliphazamiseki ngesikhathi sokuhlola.

3. Vumela ipuleti ukuthi lihlale isikhathi esinqunyiwe, ngokuvamile phakathi kwemizuzu engama-30 kuya emahoreni amaningana, kuye ngesilinganiso sokuthuthwa kwenhlabathi esifunwayo kanye nesampula ecutshungulwayo. Phakathi nalesi sikhathi, izinhlayiya ezisindayo zizohlala phansi kwemithombo kuyilapho izingxenye ezilula zizohlala zilenga noma zintanta.

4. Uma isikhathi sokuthungwa kwenhlabathi esifunekayo sesidlulile, tshekisa ipuleti kancane ukuze uthulule i-supernatant, uqaphele ukuthi ungaphazamisi inhlabathi engezansi. Qoqa izinto ezithungwe ngenhlabathi ukuze uhlaziye noma uzame okwengeziwe.

Ukuthuthukisa Ukususwa Kwendle Ngokulinganisa Okwenziwe Ekhaya

Nakuba ukuthuthwa kwenhlabathi okusizwa amandla adonsela phansi kungaveza imiphumela eyanelisayo, ngezinye izikhathi kunesidingo sokuhlukaniswa okunembe kakhudlwana, ikakhulukazi lapho kubhekene nobuningi bezinhlayiya ezahlukene. Ezimweni ezinjalo, ukulingana okwenziwe ekhaya kungasetshenziswa ukuthuthukisa ukuthuthwa kwenhlabathi. Nansi indlela yokukudala nokuyisebenzisa:

1. Thatha induku yensimbi ende, encane, neqondile (njengesigxobo sejazi) bese uyigoba ibe yisimo "L". Uhlangothi olufushane lwenduku kufanele lube lude ngokwanele ukufinyelela phansi kwemithombo epuletini lakho lokukhulisa.

2. Namathisela into encane enesisindo noma esindayo, njenge-nut yensimbi, ekugcineni okufushane kwenduku. Qinisekisa ukuthi isisindo esiphelele sihambisana nezidingo ezithile zamandla adonsela phansi okuhlolwa kwakho.

3. Beka ngokucophelela ingxenye yenduku enesisindo emthonjeni ngamunye, uqiniseke ukuthi ayiphazamisi isampula noma ithinte izindonga zepuleti lokukhulisa.

4. Vumela ipuleti lihlale lingaphazanyiswa isikhathi esifunekayo sokuthuthwa kwenhlabathi. Ukulingana okuphambene kuzothuthukisa inqubo yokuthuthwa kwenhlabathi ngokusebenzisa amandla adonsela phansi, kuhlukanise ngempumelelo izinhlayiya zobukhulu obuhlukene.

5. Ngemva kokuba isikhathi sokuthungwa kwenhlabathi sesidlulile, susa ngokucophelela ibhalansi eyenziwe ekhaya emthonjeni ngamunye bese uqhubeka nokuqoqa izinto ezithungwe ngenhlabathi ukuze uhlaziye kabanzi.

Ukusebenzisa Amandla E-Centrifugal Usebenzisa I-Swing

Indlela entsha yokulingisa i-centrifugation ihilela ukusebenzisa i-swing noma into efanayo engakhiqiza amandla e-centrifugal. Nansi indlela yokusebenzisa le ndlela:

1. Thola i-swing efanele, uqinisekise ukuthi iqinile futhi ibambeke kahle.

2. Namathisela ipuleti lakho lokukhulisa ngokuphephile ku-swing, ulibeke ngendlela evumela ukuthi okuqukethwe umthombo ngamunye kuhlakazeke ngokulinganayo ngesikhathi sokujika. Ungabeka ipuleti usebenzisa i-adhesive tape noma ama-clamp.

3. Qala ukunyakaza kokujika bese ukhuphula kancane kancane ubukhulu kanye nesivinini, ugcine isigqi esizinzile isikhathi esithile esinqunyiwe. Amandla e-centrifugal akhiqizwa yi-swing azokhuthaza ukuthuthwa kwezinhlayiya, alingise imiphumela ye-centrifugation.

4. Uma isikhathi sokuthuthwa kwenhlabathi esifunekayo sesidlulile, susa ngokucophelela ipuleti lokukhulisa kusukela ekujikeni bese uqhubeka nokuqoqwa nokuhlaziywa kwezinto ezithuthwe yinhlabathi, njengoba kudingeka.

Ukuthuthukisa kanye Nokucatshangelwa Kwamasu Okusebenza Ngesandla E-Centrifugation

Uma usebenzisa amasu ezandla, kubalulekile ukucabangela izici ezithile ukuqinisekisa imiphumela ethembekile nephindaphindwayo:

1. Qonda imikhawulo yendlela ekhethiwe bese uhlola ukufaneleka kwayo kuhlelo lwakho lokusebenza oluthile.

2. Lungiselela isikhathi nezimo zokucutshungulwa kwenhlabathi ngokwenza izivivinyo zokuqala. Shintsha iziguquguquko ezifana nesivinini sokujika, isikhathi sokucutshungulwa kwenhlabathi, kanye nevolumu yesampula ukuze kufezwe ukuhlukaniswa okuhle kakhulu.

3. Gcina izinga lokushisa nomswakama kufana phakathi nokuhlolwa, njengoba lezi zici zingathinta amazinga okusabalala kwenhlabathi.

4. Yenza izilawuli ezifanele ukuze ubhekele noma yikuphi ukubandlulula noma umehluko phakathi kwezindlela zokuxuba ngesandla kanye nezindlela zokusebenzisa umshini.

Ngokucabanga ngokucophelela ngalezi zici, abacwaningi bangasebenzisa ngempumelelo amasu okusebenzisa i-centrifugation ngesandla ukuze bafinyelele ekuhlukaniseni nasekugxiliseni izinto ngaphakathi kwamapuleti okukhulisa, ngisho noma kungekho mshini we-centrifuge.