indlela yokwenza ipleyiti yokukhulisa inkcubeko ye-centrifuge ngaphandle komatshini
Yintoni iCentrifugation kunye nokubaluleka kwayo kwiNkcubeko yeSeli?
I-Centrifugation yinkqubo ebalulekileyo kuphando lwesayensi kunye neenkqubo zelebhu, ngakumbi kwicandelo lenkcubeko yeseli. Isetyenziselwa ukwahlula izinto ezahlukeneyo zomxube ngokusekelwe kubuninzi bazo kunye namazinga okutsalwa kwetyuwa. Ngayo, abaphandi bayakwazi ukwahlula nokugxila iiseli, ii-organelles, iiproteni, kunye nezinye iinxalenye ezinomdla ukuze kuhlalutywe okanye kwenziwe uvavanyo olongezelelweyo.
Nangona kunjalo, kwiimeko ezithile, ukufikelela kumatshini we-centrifuge kunokunqongophala okanye kungafumaneki. Kwiimeko ezinjalo, kuba yimfuneko ukuphonononga ezinye iindlela zokufezekisa iziphumo ezifana ne-centrifugation kwiipleyiti zenkcubeko. Eli nqaku liza kujonga kwiindlela ezahlukeneyo kunye namaqhinga okufaka ipleyiti yenkcubeko ngaphandle komatshini, libonelela ngeengcebiso eziluncedo kubaphandi kunye nososayensi abajongene neemeko ezinjalo.
Ukusebenzisa iSedimentation encediswa yiGravity
Ukufakwa kwe-sedimentation okuncediswa ngumxhuzulane yindlela elula nesebenzayo enokulinganisa inkqubo ye-centrifugation ngaphandle kwesidingo sezixhobo ezikhethekileyo. Ukuze wenze le ndlela, landela la manyathelo:
1. Lungisa ipleyiti yakho yokukhulisa izityalo ngokubeka isampuli ngononophelo kwiqula ngalinye. Qinisekisa ukuba umthamo wolwelo uyafana kuyo yonke iqula ukuze ufumane iziphumo ezichanekileyo.
2. Beka ipleyiti yokukhulisa izityalo kwindawo ezinzileyo nethe tyaba, uqinisekise ukuba ayiphazanyiswa ngexesha lovavanyo.
3. Vumela ipleyiti ihlale ixesha elithile, ngesiqhelo phakathi kwemizuzu engama-30 ukuya kwiiyure ezininzi, kuxhomekeke kwisantya sokutsalwa kwenkunkuma esifunekayo kunye nesampuli ecutshungulwayo. Ngeli xesha, amasuntswana anzima aya kuhlala ezantsi kwemigodi ngelixa izinto ezikhaphukhaphu ziya kuhlala zixhonyiwe okanye zidada.
4. Nje ukuba ixesha elifunekayo lokuthulula inkunkuma lidlule, jika ipleyiti kancinci ukuze uthulule inkunkuma engaphezulu, uqaphele ukuba ungayiphazamisi inkunkuma esezantsi. Qokelela izinto ezithuliweyo ukuze uhlalutye okanye uzame ngakumbi.
Ukuphucula ukuthuthwa kwenkunkuma nge-Counterbalance eyenziwe ekhaya
Nangona ukutsalwa kwenkunkuma okuncediswa ngumxhuzulane kunokuvelisa iziphumo ezanelisayo, ngamanye amaxesha kukho imfuneko yokwahlulahlula ngokuchanekileyo, ingakumbi xa kujongwana nobunzima obahlukeneyo beenxalenye. Kwiimeko ezinjalo, ukulingana okwenziwe ekhaya kunokusetyenziswa ukuphucula ukutsalwa kwenkunkuma. Nantsi indlela yokuyidala nokuyisebenzisa:
1. Thatha intonga yesinyithi ende, ebhityileyo, nethe tye (njenge-hanger yejazi) uze uyigobe ibe yi-"L". Icala elifutshane lentonga kufuneka libe lide ngokwaneleyo ukufikelela ezantsi kwemithombo kwipleyiti yakho yokukhulisa izityalo.
2. Ncamathisela into encinci okanye into enzima, njenge-metal nut, kwisiphelo esifutshane sentonga. Qinisekisa ukuba ubunzima obupheleleyo buhambelana neemfuno zomxhuzulane ezithile zovavanyo lwakho.
3. Beka ngononophelo isiphelo esinobunzima sentonga emthonjeni ngamnye, uqiniseke ukuba asiphazamisi isampuli okanye asichukumisi udonga lwepleyiti yokukhulisa izityalo.
4. Vumela ipleyiti ihlale ingaphazanyiswa ixesha elifunekayo lokutsalwa kwenkunkuma. Ukulingana kuya kuyiphucula inkqubo yokutsalwa kwenkunkuma ngokusebenzisa amandla adonsela phantsi komhlaba, okwahlula ngokufanelekileyo amasuntswana anobunzima obahlukeneyo.
5. Emva kokuba ixesha lokungcoliswa lidlulile, susa ngononophelo umlinganiselo ochaseneyo owenziwe ekhaya kwiqula ngalinye uze uqhubeke nokuqokelela izinto ezingcoliswe yinkunkuma ukuze uhlalutye ngakumbi.
Ukusebenzisa Amandla E-Centrifugal Usebenzisa I-Swing
Indlela entsha yokulinganisa i-centrifugation ibandakanya ukusebenzisa i-swing okanye into efanayo enokuvelisa amandla e-centrifugal. Nantsi indlela yokusebenzisa le ndlela:
1. Fumana iswingi efanelekileyo, uqinisekise ukuba iqinile kwaye ibambeke kakuhle.
2. Ncamathisela ipleyiti yakho yokukhulisa izityalo ngokuqinileyo kwiswingi, uyibeke ngendlela evumela ukuba umxholo womthombo ngamnye usasazeke ngokulinganayo ngexesha lokushukuma. Ungayibeka ipleyiti usebenzisa iteyiphu yokuncamathelisa okanye iikliphu.
3. Qalisa intshukumo yokujikajika uze unyuse kancinci i-amplitude kunye nesantya, ugcine isigqi esizinzileyo kangangexesha elithile. Amandla e-centrifugal aveliswa yi-swing aya kukhuthaza ukutsalwa kwee-particle, alinganise imiphumo ye-centrifugation.
4. Nje ukuba ixesha elifunekayo lokunyibilikisa inkunkuma lidlule, susa ngononophelo ipleyiti yokukhulisa izityalo kwi-swing uze uqhubeke nokuqokelela nokuhlaziya izinto ezinyibilikisiweyo, njengoko kufuneka.
Ukuphucula kunye nokuqwalaselwa kweendlela zokusebenzisa i-Manual Centrifugation
Xa usebenzisa iindlela ezenziwe ngesandla, kubalulekile ukuqwalasela izinto ezithile ukuqinisekisa iziphumo ezithembekileyo neziphindaphindwayo:
1. Qonda imida yendlela ekhethiweyo kwaye uvavanye ukufaneleka kwayo kwisicelo sakho esithile.
2. Lungisa ixesha kunye neemeko zokucuthwa kwenkunkuma ngokwenza uvavanyo lokuqala. Tshintsha izinto eziguquguqukayo ezifana nesantya sokujika, ixesha lokucuthwa kwenkunkuma, kunye nomthamo wesampuli ukuze ufumane ukwahlukana okufanelekileyo.
3. Gcina ubushushu kunye nomswakama zihambelana kulo lonke uvavanyo, njengoko ezi zinto zinokuchaphazela amazinga okutsalwa kwenkunkuma.
4. Yenza ulawulo olufanelekileyo ukuze kujongwane naluphi na ucalucalulo okanye umahluko phakathi kweendlela zokucoca ngesandla nezokusebenzisa umatshini.
Ngokuqwalasela ngononophelo ezi nkalo, abaphandi banokusebenzisa ngempumelelo iindlela zokucoca ngesandla ukuze bafikelele ekuhlukaniseni nasekuxinaniseni izinto ngaphakathi kweepleyiti zokukhulisa, nokuba akukho matshini wokucoca.
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