Kuthatha isikhathi esingakanani ukuhlukanisa ama-platelet ku-centrifuge

Ukuqonda Isimiso Sokuhlukaniswa Kwamaplatelet ku-Centrifugation

I-Centrifugation iyindlela esetshenziswa kabanzi emalabhorethri nasezikhungweni zezokwelapha ukuhlukanisa izingxenye ezahlukene zamasampula ezinto eziphilayo. Ukusetshenziswa okubalulekile kwale ndlela ukuhlukaniswa kwama-platelet egazini lonke. Ama-platelet ayizingxenye ezibalulekile zegazi ezibhekene nokujiya kwegazi, ukuphulukiswa kwamanxeba, kanye nokulungiswa kwezicubu. Kulesi sihloko, sizohlola inqubo yokuhlukaniswa kwama-platelet sisebenzisa i-centrifugation futhi sihlole izici ezithonya isikhathi esidingekayo salokhu kuhlukaniswa.

I-Centrifuge: Indlu Enamandla Yokuhlukaniswa Kwama-Platelet

I-centrifuge iyithuluzi elibalulekile lokuhlukaniswa kwama-platelet. Isebenza ngesimiso samandla e-centrifugal, lapho isampula iphonswa khona ngokushesha ukuze ihlukanise izingxenye zayo ngokusekelwe ekubuneni kwazo. Lapho igazi lonke lifakwa ku-centrifuge, amandla okujikeleza abangela ukuthi amangqamuzana abomvu anzima ahlale phansi, kuyilapho ama-platelet alula akha "i-buffy coat." I-buffy coat iyingqimba ephakathi kwamaseli abomvu azinzile kanye ne-plasma.

Izici Ezithinta Isikhathi Sokuhlukaniswa Kweplatelet

Izinto eziningana zithonya isikhathi esithathwayo ukuhlukanisa ama-platelet ku-centrifuge. Into eyodwa ebalulekile ijubane le-centrifuge noma ijubane lokujikeleza. Ukwandisa ijubane lokujikeleza kuthuthukisa amandla e-centrifugal, okuholela ekuhlukanisweni okusheshayo kwama-platelet. Kodwa-ke, isivinini esikhulu ngokweqile singalimaza ama-platelet noma sishintshe ukusebenza kwawo. Ngakho-ke, ukwenza ngcono ijubane lokujikeleza kubalulekile ukuze kufezwe ukuhlukaniswa okuphumelelayo ngaphandle kokubeka engcupheni ubuqotho be-platelet.

Esinye isici esithonya isikhathi sokuhlukanisa uhlobo lwe-centrifuge esetshenziswayo. Kunezinhlobo ezahlukene, njenge-fixed-angle kanye ne-swinging-bucket centrifuges, ngayinye inemiklamo ehlukile, ukwakheka kwe-rotor, kanye nesivinini esiphezulu. Ukukhethwa kwe-centrifuge efanele kuncike ezidingweni ezithile zenqubo yokuhlukanisa, kufaka phakathi isivuno seplatelet esifiselekayo kanye nekhwalithi.

Ivolumu Yesampula kanye Nomthamo We-Centrifuge

Umthamo wesampula ecutshungulwayo ungenye into ebalulekile enquma isikhathi sokuhlukaniswa. Amavolumu amakhulu esampula ngokuvamile adinga isikhathi esengeziwe sokuhlukaniswa kwama-platelet. Ama-Centrifuge eza namakhono ahlukene, futhi ukukhetha i-centrifuge efanele ngokusekelwe kusayizi wesampula olindelwe kungenza inqubo yokuhlukaniswa ibe ngcono. Ukulayisha ngokweqile i-centrifuge ngevolumu eyengeziwe kungaholela ekuhlukanisweni okungaphelele noma ekulahlekelweni kwesampula, kuyilapho ukusebenzisa i-centrifuge encane kungandisa isikhathi sokuhlukaniswa noma kulimaze ithuluzi.

Ithonya Lokushisa Kwe-Centrifuge Ekuhlukanisweni Kwama-Platelet

Ama-Centrifuge aphinde anikeze amandla okulawula izinga lokushisa, okuvumela abasebenzisi ukuthi benze ukuhlukaniswa emazingeni okushisa athile. Izinga lokushisa elikhethiwe lokuhlukaniswa kwama-platelet lincike emphumeleni ofiselekayo. Ukupholisa igumbi le-centrifuge kungasiza ekulondolozeni ukuphila kwama-platelet, ikakhulukazi ezimweni lapho kudingeka khona ukugcinwa noma ukucubungula okulandelayo. Kodwa-ke, amazinga okushisa aphansi angandisa i-viscosity yesampula, okuthinta ukusebenza kahle kokuhlukaniswa. Ngakho-ke, ukuthola ibhalansi efanele phakathi kokulawula izinga lokushisa kanye nesivinini sokuhlukaniswa kubalulekile.

Ukwehluka Kwesicelo Nokwenza Kusebenze Kahle

Ukuhlukaniswa kwamaplatelet nge-centrifugation kuthola izinhlelo zokusebenza eziningi ocwaningweni, ekuxilongweni kwemitholampilo, kanye nasezinqubweni zokwelapha. Kuye ngokusetshenziswa okuhlosiwe, izinqubo zingashintshwa ukuze kuthuthukiswe ukukhiqizwa kwamaplatelet kanye nobumsulwa. Isibonelo, abacwaningi abafunda nge-physiology yamaplatelet bangagxila ekutholeni isivuno esiphezulu ngaphandle kwezidingo eziqinile zobumsulwa, kanti ochwepheshe bezokwelapha abenza ukumpontshelwa kwamaplatelet babeka phambili ubumsulwa kanye nokungabi nanzalo kunokukhiqiza.

Ekuphetheni, i-centrifugation iyithuluzi elinamandla lokuhlukanisa ama-platelet egazini lonke, okwenza kube lula ukuwahlukanisa ukuze kusetshenziswe izinhlelo ezahlukene ezansi. Isikhathi sokuhlukaniswa sithonywa yizici ezifana nesivinini se-centrifuge, ivolumu yesampula, umthamo we-centrifuge, izinga lokushisa, kanye nezidingo ezithile zokusetshenziswa. Ngokuqonda lezi ziguquguquko kanye nokwenza ngcono inqubo ye-centrifugation, abacwaningi kanye nochwepheshe bezempilo bangafinyelela ukuhlukaniswa kwama-platelet okusebenzayo nokuthembekile.