Kuthatha ixesha elingakanani kwi-centrifuge ukwahlula iiplatelets
Ukuqonda uMgaqo woKwahlulwa kwePlatelet kwiCentrifugation
I-Centrifugation yindlela esetyenziswa kakhulu kwiilabhoratri nakwizibonelelo zonyango ukwahlula iinxalenye ezahlukeneyo zeesampuli zebhayoloji. Enye indlela ebalulekileyo yokusetyenziswa kwale ndlela kukwahlula iiplatelets kwigazi elipheleleyo. Iiplatelets zizinto ezibalulekileyo zegazi ezinoxanduva lokugalela igazi, ukuphiliswa kwamanxeba, kunye nokulungiswa kwezicubu. Kweli nqaku, siza kuphonononga inkqubo yokwahlulwa kweeplatelets sisebenzisa i-centrifugation kwaye sihlolisise izinto ezichaphazela ixesha elifunekayo lokwahlulwa.
I-Centrifuge: Indlu Enamandla Yokwahlulwa Kweeplatelet
I-centrifuge sisixhobo esibalulekileyo sokwahlula iiplatelet. Isebenza ngokomgaqo wamandla e-centrifugal, apho isampuli ijikeleziswa ngokukhawuleza ukuze yahlule iinxalenye zayo ngokusekelwe kubuninzi bazo. Xa igazi lilonke lifakwa kwi-centrifuge, amandla okujikeleza abangela ukuba iiseli ezibomvu zegazi ezinzima zihlale ezantsi, ngelixa iiplatelet ezikhaphukhaphu zenza "i-buffy coat." I-buffy coat lulwaleko oluphakathi kweeseli ezibomvu zegazi ezihleliyo kunye ne-plasma.
Izinto Ezichaphazela Ixesha Lokwahlulwa Kweeplatelet
Izinto ezininzi zichaphazela ixesha elithathwayo ukwahlula iiplatelets kwi-centrifuge. Into ebalulekileyo yisantya se-centrifuge okanye isantya sokujikeleza. Ukwandisa isantya sokujikeleza kwandisa amandla e-centrifugal, nto leyo ekhokelela ekuqhekekeni kweeplatelets ngokukhawuleza. Nangona kunjalo, isantya esiphezulu kakhulu singonakalisa iiplatelets okanye sitshintshe ukusebenza kwazo. Ke ngoko, ukuphucula isantya sokujikeleza kubalulekile ukuze kufezekiswe ukwahlukana okusebenzayo ngaphandle kokubeka emngciphekweni ukuthembeka kweeplatelets.
Enye into echaphazela ixesha lokwahlulwahlulwa luhlobo lwe-centrifuge esetyenziswayo. Kukho iintlobo ezahlukeneyo, ezifana ne-fixed-angle kunye ne-swinging-bucket centrifuges, nganye ineedizayini ezahlukeneyo, ulungelelwaniso lwe-rotor, kunye nesantya esiphezulu. Ukukhethwa kwe-centrifuge efanelekileyo kuxhomekeke kwiimfuno ezithile zenkqubo yokwahlulwahlulwa, kubandakanya isivuno seplatelet esifunekayo kunye nomgangatho.
Umthamo weSampuli kunye nomthamo weCentrifuge
Umthamo wesampulu ecutshungulwayo yenye into ebalulekileyo echaza ixesha lokwahlulwa. Umthamo omkhulu wesampulu uhlala ufuna ixesha elingakumbi lokwahlulwa kweplatelet. IiCentrifuge ziza namandla ahlukeneyo, kwaye ukukhetha icentrifuge efanelekileyo ngokusekelwe kubungakanani besampulu obulindelweyo kunokuphucula inkqubo yokwahlulwa. Ukulayisha icentrifuge kakhulu ngomthamo ogqithisileyo kunokukhokelela ekuhlukanisweni okungaphelelanga okanye ekulahlekelweni kwesampulu, ngelixa ukusebenzisa icentrifuge engancinci kunokwandisa ixesha lokwahlulwa okanye konakalise isixhobo.
Impembelelo yoBushushu beCentrifuge kwiSahlulo sePlatelet
IiCentrifuge zikwabonelela ngamandla okulawula ubushushu, zivumela abasebenzisi ukuba benze ukwahlulahlula kumaqondo obushushu athile. Ubushushu obukhethiweyo bokwahlulwahlulwa kweeplatelet buxhomekeke kwisiphumo esifunekayo. Ukupholisa igumbi lecentrifuge kunokunceda ekugcineni amandla okuphila kweeplatelet, ingakumbi kwiimeko apho kufuneka ukugcinwa okanye ukucubungula okulandelayo. Nangona kunjalo, amaqondo obushushu aphantsi anokunyusa i-viscosity yesampuli, nto leyo echaphazela ukusebenza kakuhle kokwahlulwahlulwa. Ke ngoko, ukufumana ibhalansi efanelekileyo phakathi kolawulo lobushushu kunye nesantya sokwahlulwahlulwa kubalulekile.
Utshintsho lweSicelo kunye nokuLungiselela
Ukwahlulwa kweeplatelet nge-centrifugation kufumana izicelo ezininzi kuphando, ukuxilongwa kwezonyango, kunye neenkqubo zonyango. Ngokuxhomekeke kwindlela ekucetywayo ukusetyenziswa ngayo, iiprotokholi zinokuguqulwa ukuze kuphuculwe isivuno kunye nobunyulu beeplatelet. Umzekelo, abaphandi abafunda nge-physiology yeeplatelet banokugxila ekufumaneni isivuno esiphezulu ngaphandle kweemfuneko ezingqongqo zobunyulu, ngelixa iingcali zonyango ezenza utofelo-gazi lweeplatelet zibeka phambili ubunyulu kunye nobunyulu kunesivuno.
Ukuqukumbela, i-centrifugation sisixhobo esinamandla sokwahlula iiplatelets egazini liphela, nto leyo evumela ukuba zihlukaniswe kwiindlela ezahlukeneyo zokusetyenziswa. Ixesha lokwahlulwa liphenjelelwa zizinto ezifana nesantya se-centrifuge, umthamo wesampuli, umthamo we-centrifuge, ubushushu, kunye neemfuno ezithile zokusetyenziswa. Ngokuqonda ezi zinto ziguquguqukayo kunye nokwenza ngcono inkqubo ye-centrifugation, abaphandi kunye neengcali zezempilo banokufikelela ekuhlukanisweni kweeplatelets okusebenzayo nokuthembekileyo.
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