i-centrifuge ihlukanisa ihlule ku-

I-Centrifuge: Ukuhlukanisa i-Clot ne-Mix

Isingeniso ku-Centrifugation kanye ne-Blood Clotting

I-Centrifugation iyindlela eyisisekelo esetshenziswa ezinhlotsheni ezahlukene zesayensi nezokwelapha ukuhlukanisa izingxube ngokusekelwe ekubuneni kwazo. Enye yezindawo ezibalulekile lapho i-centrifugation idlala khona indima ebalulekile kusekunakekelweni kwezempilo, ikakhulukazi ukujiya kwegazi. Lesi sihloko sigxila ekubalulekeni kanye nendlela i-centrifuge esebenza ngayo ekuhlukaniseni i-clot engxubeni.

Ukuqonda Ukugoqa Igazi

Ukujiya kwegazi, noma ukujiya kwegazi, kuyinqubo eyinkimbinkimbi evimbela ukuphuma kwegazi ngokweqile lapho imithambo yegazi yonakele. Kuhilela uchungechunge lwezinyathelo ezilandelanayo lapho ama-platelet, amaseli akhethekile egazini lethu, akha i-plug endaweni yokulimala futhi abangele ukusebenza kwamaprotheni okujiya kwegazi. Kodwa-ke, kwezinye izimo zezokwelapha, ukujiya kwegazi ngokweqile kungenzeka futhi kuholele emiphumeleni emibi yezempilo. Ezimweni ezinjalo, ukuhlukanisa i-clot egazini kuba yinto edingekayo.

Isimiso Sokugqamisa I-Centrifugation

I-Centrifugation isebenzisa isimiso sokuhlukanisa izingxube ngokusekelwe ekubuneni kwazo ngokusebenzisa ukujikeleza ngesivinini esikhulu. I-centrifuge umshini ofeza le nqubo ngokujikelezisa amasampula ngesivinini esikhulu, odala amandla e-centrifugal ahlukanisa izingxenye ngokusekelwe esisindweni sazo. Emongweni wokujiya kwegazi, i-centrifuge ingasiza ekuhlukaniseni igazi elijiyile kumaseli egazi angajiyile kanye ne-plasma.

Izicelo Kwezokwelapha Nocwaningo

Indima ye-centrifuge ekunakekelweni kwezempilo idlulela ngale nje kokuhlukaniswa kwe-clot yegazi engxubeni. Ochwepheshe bezokwelapha bathembele kule ndlela ngezinhloso ezahlukene, okuhlanganisa ukuxilonga nokuqapha izifo. Ama-centrifuge asetshenziswa kakhulu emalabhorethri ukuhlukanisa izingxenye zegazi, njengeseli legazi elibomvu nelimhlophe, i-plasma, nama-platelet. Ngaphezu kwalokho, abacwaningi bathembele enkambisweni yokuhlukanisa ukuze bafunde izakhiwo zezingxenye ezithile ngokuningiliziwe.

Izinhlobo ze-Centrifuges

Ama-Centrifuge eza ngezinhlobo nobukhulu obuhlukahlukene, ngayinye yenzelwe izinhlelo zokusebenza ezithile. Izinhlobo ze-centrifuge ezisetshenziswa kakhulu zifaka phakathi ama-tabletop centrifuge, ama-microcentrifuge, kanye nama-ultracentrifuge. Ama-tabletop centrifuge mancane futhi afanele imisebenzi ejwayelekile, kuyilapho ama-microcentrifuge engamayunithi amancane asetshenziselwa ukucubungula amavolumu amancane amasampula. Ama-Ultracentrifuge, ngakolunye uhlangothi, makhulu futhi angajikeleza amasampula ngesivinini esiphezulu, okwenza kube lula ukuhlukaniswa kahle kwamavolumu amakhulu kanye nezinto ezincane.

Inqubo Yokukhuphula I-Centrifugation Yokuhlukanisa I-Clot

Ukuze kuhlukaniswe ihlule legazi engxubeni, i-centrifuge isebenzisa inqubo enezinyathelo ezimbili: i-centrifugation kanye ne-decantation. Okokuqala, isampula equkethe ihlule kanye nezingxenye ezizungezile ifakwa epayipini le-centrifuge, elibe selibekwa ngokucophelela epayipini le-centrifuge. Uma selivuliwe, i-centrifuge iqala ukujikeleza ngokushesha, ikhiqize amandla asunduzela izingxenye ezixineneyo, njengehlule, phansi kwepayipi. Izingxenye eziwuketshezi ezizungezile ziyakhuphukela phezulu.

Ukuhlanza Nokuqoqwa Kwezingxenye Ezihlukanisiwe

Ngemva kokusebenzisa i-centrifugation, isinyathelo esilandelayo ukukhipha i-supernatant (uketshezi olungaphezu kwe-clot) luthululwa ngokucophelela noma lususwe kusetshenziswa i-pipette. Le nqubo ishiya i-clot ehlukanisiwe phansi kwepayipi. I-clot ingabe isiqoqwa futhi ihlaziywe kabanzi, uma kudingeka. Ngokuhlukanisa i-clot engxubeni, abacwaningi nodokotela bangathola ukuqonda okubalulekile ngokwakheka kwe-clot, isakhiwo, kanye nokukhubazeka okungenzeka okukhona.

Intuthuko kubuchwepheshe be-Centrifugation

Eminyakeni edlule, intuthuko kwezobuchwepheshe bokusebenzisa i-centrifugation iholele ekusebenzeni kahle nasekuqondeni okuthuthukisiwe. Ama-centrifuge esimanje anezilungiselelo ezingahlelwa, okuvumela ososayensi ukuthi benze ngokwezifiso isivinini, ubude, kanye nezinga lokushisa ukuze kuhambisane nezidingo ezithile. Ngaphezu kwalokho, izici zokuphepha ezithuthukisiwe zinciphisa ingozi yokungcola kwesampula kanye nomonakalo wemishini. Lezi ntuthuko zibe negalelo elikhulu ekuthuthukisweni kocwaningo lwezokwelapha kanye nokuxilongwa kwezokwelapha.

Isiphetho

Ekuphetheni, i-centrifuge idlala indima ebalulekile ekuhlukaniseni amahlule engxubeni ezindaweni zezokwelapha nezocwaningo. Ngokusebenzisa izimiso ze-centrifugation, lobu buchwepheshe buvumela ukuqoqwa kwamasampula ahlanziwe ukuze kuhlaziywe kabanzi. Ngokuthuthuka okuqhubekayo, amasu e-centrifugation ayaqhubeka nokukhula, esiza ekuqondeni nasekwelapheni izinkinga ezahlukahlukene zokujiya kwegazi kanye nokuthuthukisa imiphumela yezempilo emhlabeni jikelele.