centrifuge inoparadzanisa ropa rakagwamba kubva

Centrifuge: Kuparadzanisa Clot kubva Musanganiswa

Nhanganyaya yeCentrifugation uye Kuvhara Ropa

Kubatanidza masero (centrifugation) inzira huru inoshandiswa mune zvakasiyana-siyana zvesainzi nezvehutano kupatsanura misanganiswa zvichienderana nehuwandu hwayo. Imwe yenzvimbo dzakakosha apo kusanganisa masero (centrifugation) kunoita basa guru muhutano, kunyanya kugonya ropa. Chinyorwa chino chinotsanangura kukosha uye mashandiro ekushandisa centrifuge mukupatsanura kugonya kubva mumusanganiswa.

Kunzwisisa Kuvhara Ropa

Kugwamba kweropa, kana kuti kugomara kweropa, inzira yakaoma inodzivirira kubuda ropa rakawanda kana tsinga dzeropa dzakuvara. Inosanganisira matanho akatevedzana apo maplatelet, masero akakosha muropa redu, anoumba chivharo panzvimbo yekukuvara uye anotanga kushanda kwemapuroteni anogwamba. Zvisinei, mune mamwe mamiriro ehutano, kugomara kwakawanda kunogona kuitika uye kutungamira kumigumisiro yakakomba kuhutano. Muzviitiko zvakadaro, kuparadzanisa kugomara kweropa kubva muropa kunova kwakakosha.

Nheyo yeCentrifugation

Kutenderera kwemasero kunoshandisa musimboti wekuparadzanisa musanganiswa zvichienderana nehuwandu hwawo nekushandisa kutenderera nekukurumidza. Kutenderera kwemasero muchina unoita izvi nekutenderedza masampuli nekumhanya kwakanyanya, zvichigadzira simba repakati rinoparadzanisa zvikamu zvichienderana nehuremu hwazvo. Panyaya yekugwamba kweropa, kutenderera kwemasero kunogona kubatsira kuparadzanisa ropa rakagwamba kubva kumasero eropa asina kugwamba uye plasma.

Mashandisirwo muMishonga neKutsvagisa

Basa re centrifuge muhutano rinopfuura kungoparadzanisa ropa rakagwamba kubva mumusanganiswa. Nyanzvi dzezvekurapa dzinoshandisa nzira iyi pazvinangwa zvakasiyana-siyana, kusanganisira kuongorora nekutarisa zvirwere. Centrifuge dzinoshandiswa zvakanyanya mumarabhoritari kuparadzanisa zvikamu zveropa, zvakaita semasero matsvuku nemachena eropa, plasma, uye maplatelet. Pamusoro pezvo, vaongorori vanovimba nenzira yekuparadzanisa kuti vadzidze hunhu hwezvikamu zvakati wandei zvakadzama.

Mhando dzeCentrifuges

MaCentrifuge anouya mumhando dzakasiyana-siyana uye saizi, imwe neimwe yakagadzirirwa mashandisirwo chaiwo. Mhando dzeCentrifuge dzinoshandiswa zvakanyanya dzinosanganisira maCentrifuge etabletop, maMicrocentrifuge, uye maUltracentrifuge. MaCentrifuge etabletop madiki uye akakodzera mabasa enguva dzose, nepo maMicrocentrifuge ari mayuniti madiki anoshandiswa kugadzirisa mavhoriyamu madiki emasampuli. MaUltracentrifuge, kune rumwe rutivi, akakura uye anogona kutenderedza masampuli nekumhanya kwakanyanya, zvichiita kuti kupatsanurwa kwemavhoriyamu makuru uye zvidimbu zvishande zvakanaka.

Maitiro eCentrifugation ekuparadzanisa mavanga

Kuti ropa riparadzaniswe kubva mumusanganiswa, centrifuge inoshandisa matanho maviri: centrifugation uye clearing. Kutanga, sampuro ine clot nezvinhu zvakaipoteredza inoiswa muchubhu yecentrifuge, iyo inozoiswa zvakanaka mucentrifuge. Kana yabatidzwa, centrifuge inotanga kutenderera nekukurumidza, ichigadzira simba rinosundira zvikamu zvakawandisa, zvakaita seclot, pasi pechubhu. Zvikamu zvemvura zvakaipoteredza zvinokwira kumusoro.

Kucheneswa uye Kuunganidzwa kweZvikamu Zvakaparadzaniswa

Mushure mekuparadzanisa ropa, danho rinotevera nderekubvisa ropa, uko supernatant (mvura iri pamusoro pegwamba) inodururwa kana kubviswa nokungwarira uchishandisa pipette. Maitiro aya anosiya gwamba rakaparadzaniswa riri pasi pechubhu. Gwamba rinogona kuunganidzwa uye kuongororwazve, kana zvichidikanwa. Nekuparadzanisa gwamba kubva mumusanganiswa, vaongorori nevanachiremba vanogona kuwana ruzivo rwakakosha pamusoro pemagadzirirwo egwamba, chimiro, uye kusarongeka kunogona kuitika.

Kufambira Mberi muTekinoroji yeCentrifugation

Mumakore apfuura, kufambira mberi muhunyanzvi hwe centrifugation kwakatungamira mukuvandudzwa kwekushanda zvakanaka uye kururama. Masentifuga emazuva ano ane marongero anogona kurongwa, zvichibvumira masayendisiti kugadzirisa kumhanya, nguva, uye tembiricha kuti zvienderane nezvinodiwa chaizvo. Pamusoro pezvo, maficha ekuchengetedza epamusoro anoderedza njodzi yekusvibiswa kwemuenzaniso uye kukuvara kwemidziyo. Izvi zvakabatsira zvikuru mukufambira mberi mukutsvaga kwezvokurapa uye kuongororwa kwekiriniki.

Mhedziso

Mukupedzisa, centrifuge inoita basa guru mukuparadzanisa mavanga kubva mumusanganiswa wemishonga neongororo. Nekushandisa misimboti ye centrifugation, tekinoroji iyi inogonesa kuunganidzwa kwemasampuli akacheneswa kuti awedzerwe kuongororwa. Nekufambira mberi kuri kuitika, matekiniki e centrifugation ari kuramba achichinja, zvichibatsira mukunzwisisa nekurapa zvirwere zvakasiyana-siyana zvekugwamba kweropa uye kuvandudza mhedzisiro yehutano pasi rese.