i-centrifuge isetshenziselwa ukuhlukanisa imibuzo

Isingeniso kuma-Centrifuge kanye nendima yawo ezinqubweni zokuhlukanisa

Ama-centrifuge adlala indima ebalulekile ezimbonini ezahlukene, emalabhorethri, nasezikhungweni zocwaningo ngokuhlinzeka ngendlela yokuhlukanisa izingxenye ezahlukene zengxube ngokusekelwe ekubuneni kwazo. Njengoba enekhono lokukhiqiza amandla amakhulu adonsela phansi, ama-centrifuge avumela ososayensi nonjiniyela ukuthi bahlukanise izinhlayiya noma izinto ezithile ukuze zihlaziywe noma zisetshenziswe kabanzi. Kulesi sihloko, sizohlola izimiso eziyisisekelo ze-centrifugation futhi sihlole ukusetshenziswa kwayo emikhakheni eyahlukahlukene.

Ukuqonda Isimiso Sokugqamisa I-Centrifugation

I-Centrifugation inqubo esebenzisa isimiso sokuthuthwa kwenhlabathi, lapho izinhlayiya noma izingxenye ezisindayo zihlala khona ngokushesha ngaphansi kwethonya lamandla adonsela phansi. I-centrifuge iyithuluzi elisebenza ngomshini elijikeleza into noma isampula ngesivinini esikhulu, lidala amandla e-centrifugal adlula amandla adonsela phansi. La mandla abangela ukuthi izinhlayiya ezixineneyo zithuthele emngceleni kuyilapho ezikhanyayo zihlala maphakathi, okwenza kube lula ukuhlukana kwazo.

Izingxenye kanye Nendlela Yokusebenza Ye-Centrifuge

I-centrifuge evamile iqukethe izingxenye eziningana ezibalulekile, okuhlanganisa i-rotor, i-motor, kanye ne-control unit. I-rotor, evame ukwenziwa ngezinto eziqinile njenge-titanium, iyisici esiyinhloko esibamba amashubhu noma izitsha zesampula. I-motor, esebenza ngogesi, inikeza ukunyakaza kokujikeleza okudingekayo ukuze kujikelezwe i-rotor. Okokugcina, i-control unit ivumela umsebenzisi ukuthi asethe isivinini nesikhathi esifunekayo senqubo yokuhlukanisa.

Uma i-centrifuge ivuliwe, injini ibangela ukuthi i-rotor ijikeleze ngokushesha, ikhiqize amandla e-centrifugal. Ngenxa yalokho, isampula ngaphakathi kwamapayipi ithola amandla angaphandle asunduza izinhlayiya ezixineneyo ngaphandle, ngokumelene nezindonga zamapayipi. Ngesikhathi esifanayo, izingxenye ezikhanyayo zihlala ziseduze nesikhungo ngenxa yobuningi bazo obuphansi. Lokhu kuhlukaniswa kwenzeka ngenxa yamazinga okuhlukaniswa kwe-sedimentation, okwenza izingxenye ezihlukile ziqoqwe ngokwehlukana.

Ukusetshenziswa kwe-Centrifuges embonini nasocwaningweni

Ama-Centrifuge athola izinhlelo zokusebenza eziningi kuzo zonke izimboni kanye nezizinda zocwaningo. Emkhakheni wezokwelapha, asetshenziselwa ukuhlukanisa izingxenye zegazi, njengamaseli abomvu egazi, i-plasma, nama-platelet, okwenza kube lula ukucutshungulwa kweminikelo yegazi noma ukuxilongwa. Izinkampani zemithi zisebenzisa amasu okuhlukanisa amakhemikhali athile, ukuhlanza imithi, noma ukukhipha izingxenye ezifunwayo emithonjeni yezinto eziphilayo.

Izimboni zamakhemikhali nezokudla zisebenzisa ama-centrifuge ukuze zicwenge futhi zihlanze izinto ezifana noshukela, uwoyela, namakhemikhali. Ngokususa ukungcola noma ukuhlukanisa izakhi ezahlukene, i-centrifuge iyasiza ekuqinisekiseni ikhwalithi ephezulu yomkhiqizo. Abacwaningi abafunda amaprotheni, i-DNA, i-RNA, noma izinhlayiya ezingaphansi kweseli bathembele ku-ultracentrifugation, uhlobo oluthuthukisiwe lwe-centrifugation, ukuze bahlukanise futhi bahlaziye lezi zingxenye, basize ekuqondeni izinqubo zezinto eziphilayo.

Izinto Okufanele Uzicabangele Lapho Ukhetha I-Centrifuge Nokuqinisekisa Ukusebenza Okuphephile

Uma ukhetha i-centrifuge, izici eziningana kumele zicatshangelwe, okuhlanganisa ububanzi besivinini, umthamo omkhulu, kanye nezinhlelo zokusebenza ezithile ezizosetshenziselwa zona. Kunezinhlobo ezahlukene zama-centrifuge, njenge-microcentrifuge, ama-benchtop centrifuge, kanye nama-centrifuge emodeli yephansi, ngalinye lihlangabezana nezidingo ezahlukene. Izinyathelo zokuphepha, njengezindlela zokukhiya izivalo, ukutholwa kokungalingani, kanye nezihlangu zokuvikela, nazo zibalulekile ekuvimbeleni izingozi nokuqinisekisa ukuphepha komqhubi.

Ekuphetheni, ama-centrifuge angamathuluzi abalulekile ezinqubweni zokuhlukanisa, asetshenziswa kabanzi kuzo zonke izimboni kanye nemikhakha yocwaningo. Ngokusebenzisa amandla e-centrifugal, lawa madivayisi avumela ukuhlukaniswa nokukhishwa kwezingxenye ezithile ezixutshweni, okuqhubekisela phambili intuthuko yesayensi neyezimboni. Ukuqonda izimiso ze-centrifugation, ukukhetha idivayisi efanele, kanye nokunamathela ezimisweni zokuphepha kubalulekile ekusebenzeni kwe-centrifuge okuphumelelayo nokuphephile.