i-centrifuge isetyenziselwa ukwahlula i-quizlet
Intshayelelo kwiCentrifuges kunye nendima yazo kwiinkqubo zokwahlukana
Ii-centrifuge zidlala indima ebalulekileyo kumashishini ahlukeneyo, kwiilabhoratri, nakwizibonelelo zophando ngokubonelela ngeendlela zokwahlula izinto ezahlukeneyo zomxube ngokusekelwe kubuninzi bazo. Ngenxa yokukwazi kwazo ukuvelisa amandla amakhulu omxhuzulane, ii-centrifuge zivumela izazinzulu kunye neenjineli ukuba zahlule amasuntswana athile okanye izinto ukuze zihlalutywe okanye zisetyenziswe ngakumbi. Kweli nqaku, siza kujonga imigaqo esisiseko ye-centrifugation kwaye sihlolisise ukusetyenziswa kwayo kwiinkalo ezahlukeneyo.
Ukuqonda uMgaqo weCentrifugation
I-Centrifugation yinkqubo esebenzisa umgaqo we-sedimentation, apho amasuntswana anzima okanye izinto ezihlala phantsi ngokukhawuleza phantsi kwempembelelo yomxhuzulane. I-centrifuge sisixhobo soomatshini esijikeleza into okanye isampuli ngesantya esiphezulu, senza amandla e-centrifugal adlula amandla omxhuzulane. La mandla abangela ukuba amasuntswana axineneyo afudukele ecaleni ngelixa lawo alula ehlala embindini, nto leyo eququzelela ukwahlukana kwawo.
Izinto ezisetyenziswayo kunye nendlela yokusebenza yeCentrifuge
I-centrifuge eqhelekileyo ineenxalenye ezibalulekileyo ezininzi, kuquka i-rotor, i-motor, kunye neyunithi yolawulo. I-rotor, edla ngokwenziwa ngezinto ezihlala ixesha elide ezifana ne-titanium, yinto ephambili ebamba iityhubhu okanye izikhongozeli zesampulu. I-motor, eqhutywa ngumbane, inika intshukumo yokujikeleza efunekayo ekujikeleziseni i-rotor. Okokugqibela, iyunithi yolawulo ivumela umsebenzisi ukuba amisele isantya esifunekayo kunye nexesha lenkqubo yokwahlula.
Xa i-centrifuge ivuliwe, i-motor ibangela ukuba i-rotor ijikeleze ngokukhawuleza, ivelise amandla e-centrifugal. Ngenxa yoko, isampuli engaphakathi kwiityhubhu ifumana amandla angaphandle atyhala amasuntswana axineneyo ngaphandle, ngokuchasene neendonga zeetyhubhu. Kwangaxeshanye, izinto ezikhaphukhaphu zihlala zikufutshane nombindi ngenxa yoxinano lwazo oluphantsi. Olu kwahlukana lwenzeka ngenxa yamazinga okwahlukana kwe-sedimentation, okuvumela ukuba izinto ezahlukeneyo ziqokelelwe ngokwahlukeneyo.
Ukusetyenziswa kweeCentrifuges kwiShishini noPhando
IiCentrifuge zifumana usetyenziso olubanzi kumashishini ahlukeneyo nakwiindawo zophando. Kwicandelo lezonyango, zisetyenziselwa ukwahlula iinxalenye zegazi, ezifana neeseli ezibomvu zegazi, iplasma, kunye neeplatelets, okuvumela ukucutshungulwa kweminikelo yegazi okanye ukuxilongwa. Iinkampani zamayeza zisebenzisa iindlela ze-centrifugation ukwahlula iikhompawundi ezithile, ukucoca amayeza, okanye ukukhupha iinxalenye ezifunekayo kwimithombo yebhayoloji.
Amashishini eekhemikhali nokutya asebenzisa ii-centrifuge ukuze acoce aze acoce izinto ezifana neeswekile, iioyile, kunye neekhemikhali. Ngokususa ukungcola okanye ukwahlula izinto ezahlukeneyo, i-centrifuge inceda ekuqinisekiseni umgangatho ophezulu wemveliso. Abaphandi abafunda iiproteni, i-DNA, i-RNA, okanye amasuntswana angaphantsi kweseli baxhomekeke kwi-ultracentrifugation, uhlobo oluphambili lwe-centrifugation, ukuze bahlukanise baze bahlalutye ezi zinto, benceda ekuqondeni iinkqubo zebhayoloji.
Izinto ekufuneka ziqwalaselwe xa ukhetha iCentrifuge kwaye uqinisekisa ukusebenza ngokukhuselekileyo
Xa ukhetha i-centrifuge, kufuneka kuqwalaselwe izinto ezininzi, kuquka isantya, amandla aphezulu, kunye nezicelo ezithile eziza kusetyenziselwa zona. Iindidi ezahlukeneyo ze-centrifuge ziyafumaneka, njenge-microcentrifuges, i-benchtop centrifuges, kunye ne-floor-model centrifuges, nganye ihlangabezana neemfuno ezahlukeneyo. Amanyathelo okhuseleko, anjengeendlela zokuvala izivalo, ukufumanisa ukungalingani, kunye nezikhuselo zokukhusela, nazo zibalulekile ekuthinteleni iingozi nokuqinisekisa ukhuseleko lwabaqhubi.
Ukuqukumbela, ii-centrifuge zizixhobo ezibalulekileyo kwiinkqubo zokwahlulahlula, ezisetyenziswa kakhulu kumashishini nakwiindawo zophando. Ngokusebenzisa amandla e-centrifugal, ezi zixhobo zivumela ukwahlulwa nokukhutshwa kwezinto ezithile kwimixube, nto leyo eqhubela phambili inkqubela phambili yesayensi neyemizi-mveliso. Ukuqonda imigaqo ye-centrifuge, ukukhetha isixhobo esifanelekileyo, kunye nokunamathela kwimigaqo yokhuseleko kubalulekile ekusebenzeni kakuhle nangokukhuselekileyo kwe-centrifuge.
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