yeyiphi i-centrifugal force esetyenziswayo ukwahlula igazi
Intshayelelo
Amandla e-centrifugal yingcamango esisiseko kwifiziksi edlala indima ebalulekileyo kumashishini ahlukeneyo, kuquka amayeza kunye nokhathalelo lwempilo. Kwicandelo lokuxilongwa kwezonyango, amandla e-centrifugal asetyenziselwa ukwahlula iinxalenye zegazi ukwenza iimvavanyo ezahlukeneyo, ezinje ngokubalwa kweeseli zegazi, uhlobo lwegazi, kunye nohlalutyo lwee-chemical markers ezahlukeneyo. Eli nqaku lijolise ekuchazeni ukuba yintoni amandla e-centrifugal, indlela aveliswa ngayo, kunye nendlela asetyenziswa ngayo ukwahlula iinxalenye zegazi ngokufanelekileyo.
1. Ukuqonda Amandla Aphakathi
Amandla e-Centrifugal ngamandla angeyonyani abonakala esebenza emzimbeni ohamba ngendlela ejikelezayo. Nangona kubonakala ngathi amandla atsala izinto kude nombindi wokujikeleza, eneneni, ngamandla angaphandle abangelwa kukungasebenzi kakuhle kwento. Njengoko igazi liyinto elulwelo, lilandela imigaqo yokuguquguquka kolwelo, okwenza ukuba lufaneleke ukwahlukana kusetyenziswa amandla e-centrifugal.
2. Iimashini zeCentrifuge: Ukujonga Ngokusondeleyo
Iimashini zeCentrifuge zizixhobo ezikhethekileyo ezisetyenziswa kwiindawo ezahlukeneyo zonyango ukwahlula iinxalenye zegazi. Ezi mashini zisebenza ngokomgaqo wamandla ecentrifugal kwaye ziqulathe indibano yerotor ejikeleza ngesantya esiphezulu. Isampuli yegazi ifakwa kwiityhubhu okanye kwiikhonteyina ngaphakathi kwerotor kwaye ijikelezwe ngamandla, nto leyo ekhokelela ekuhlukanisweni kweenxalenye zayo ezahlukeneyo.
3. Ukwakheka Kwegazi
Ngaphambi kokuba sijonge inkqubo yokwahlulwa kwegazi kusetyenziswa amandla e-centrifugal, kubalulekile ukuqonda ukwakheka kwegazi. Igazi lenziwe ngamacandelo ahlukeneyo, kuquka iiseli ezibomvu zegazi (ii-erythrocytes), iiseli ezimhlophe zegazi (ii-leukocytes), iiplatelets (ii-thrombocytes), kunye ne-plasma. La macandelo anobunzima nobukhulu obahlukeneyo, nto leyo eyenza ukuba ahlukaniswe nge-centrifugation.
4. Inkqubo Yokwahlulwa Kwegazi
Inkqubo yokwahlulwa kwegazi ibandakanya amanyathelo aliqela ukuqinisekisa ukwahlulwa okusebenzayo kwamacandelo ayo. Okokuqala, isampuli yegazi iqokelelwa kwisigulana kusetyenziswa isirinji okanye ityhubhu yokuqokelela ekhethekileyo. Okulandelayo, isampuli idluliselwa ngononophelo kwiityhubhu ezenzelwe ngokukodwa ukucocwa. Ezi tyhubhu zihlala ziphawulwa ngesantya esiphezulu apho zinokucocwa khona ukuze kuthintelwe umonakalo.
5. Ukukhetha isantya kunye nexesha elifanelekileyo leCentrifuge
Ukuze kufezekiswe ukwahlulwahlulwa ngokugqibeleleyo kwezakhi zegazi, kubalulekile ukukhetha isantya esifanelekileyo kunye nobude bexesha lokufakwa kwe-centrifugation. Amacandelo egazi ahlukeneyo anobunzima nobukhulu obahlukeneyo, nto leyo ebangela ukuba kubekho iiparameter ezahlukeneyo zokufakwa kwe-centrifugation. Umzekelo, kwimeko yokubala iiseli zegazi ezisisiseko, isantya esiphantsi kunye nexesha elifutshane kunokwanela, ngelixa uvavanyo oluntsonkothileyo lunokufuna isantya esiphezulu kunye nobude bexesha elide.
6. Inkqubo Yokwahlukana
Nje ukuba iityhubhu ezinesampuli yegazi zibekwe ngokukhuselekileyo kwi-centrifuge, i-rotor iqala ukujikeleza ngokukhawuleza. Intshukumo yokujikeleza idala amandla aphakathi atyhala iinxalenye zegazi ezixineneyo ziye ezantsi kwetyhubhu, ngelixa iinxalenye ezingaxineneyo zihlala zisondelelene nephezulu. Ngale ndlela, iinxalenye zegazi zahlukana zibe ziingqimba ezahlukeneyo, ezinokufikelelwa lula kwaye zihlalutywe.
7. Ukufunyanwa nokuHlalutywa kweeCandelo zeGazi ezaHluliweyo
Emva kokuba inkqubo ye-centrifugation igqityiwe, iinxalenye zegazi ezahlulweyo zingafunyanwa ngononophelo kusetyenziswa i-pipette okanye ezinye izixhobo ezikhethekileyo. Iileya, kuquka iiseli ezibomvu zegazi ezipakishwe ezantsi, i-buffy coat (umaleko omncinci weeseli ezimhlophe zegazi kunye neeplatelets), kunye ne-plasma ephezulu, emva koko zijongwa ngakumbi kwaye zivavanywe.
Isiphelo
Amandla e-centrifugal ngumgaqo obalulekileyo osetyenziswa kwiilabhoratri zonyango nakwizibonelelo zempilo ukuze kwahlulwe iinxalenye zegazi ngokufanelekileyo. Ngokusebenzisa amandla e-centrifugation, iingcali zonyango zinokufumana ulwazi oluxabisekileyo malunga nempilo yesigulana kwaye zenze uxilongo oluchanekileyo. Ukuqonda indlela amandla e-centrifugal asebenza ngayo kunye nokusetyenziswa kwawo ekuhlukaniseni igazi kwenza oogqirha bakwazi ukubonelela ngononophelo olungcono lwesigulana kwaye baphucule iziphumo zempilo.
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