indlela yokwahlula i-serum egazini nge-centrifuge
Intshayelelo:
IiCentrifuge zidlala indima ebalulekileyo kwiinkalo ezahlukeneyo zesayensi nezonyango, zivumela abaphandi kunye noogqirha ukuba bahlule iinxalenye zomxube ngokusekelwe kubuninzi bazo. Enye indlela ephawulekayo yokusebenzisa i-centrifuge kukwahlula i-serum egazini. Le nkqubo inika ulwazi oluxabisekileyo kwimpilo yesigulana ngokwahlula nokuhlalutya iiproteni, ii-enzymes, iihomoni, kunye nezinye iinxalenye ezikhoyo kwi-serum. Kweli nqaku, siza kuhlola inkqubo yokwahlula i-serum egazini sisebenzisa i-centrifuge, sigqamisa ukubaluleka kwayo ekuxilongeni, kuphando, nakwizicelo zonyango.
Ukuqonda i-Centrifugation:
I-Centrifugation yindlela apho amandla e-centrifugal asetyenziswa khona ukwahlula izinto ezinobunzima obahlukeneyo. Ngokujikelezisa isampuli ngesantya esiphezulu, i-centrifugation ibangela ukuba amasuntswana axineneyo ahlale ezantsi, enze i-pellet, ngelixa izinto ezikhaphukhaphu zihlala kwi-supernatant. Ukuze kufezekiswe ukwahlukana okuphumelelayo, kubalulekile ukuqonda imigaqo esisiseko kunye nezinto ezichaphazela i-centrifugation.
Ukukhetha iCentrifuge efanelekileyo:
Inyathelo lokuqala lokwahlula i-serum egazini kukukhetha i-centrifuge efanelekileyo. Ii-centrifuge ezahlukeneyo zibonelela ngezakhono ezahlukeneyo, ezinje ngobukhulu obahlukeneyo be-rotor, isantya esiphezulu, kunye noseto olucwangcisiweyo. Kwi-serum serum, i-centrifuge ene-rotor ejikayo idla ngokukhethwa, njengoko ivumela ukuqokelelwa kwezinto ezahlulweyo ngaphandle kokuphazamisa i-pellet. Ukongeza, qaphela amandla aphezulu e-centrifuge e-g-force, kwaye uqinisekise ukuba iyahlangabezana neemfuno zokwahlula i-serum.
Ukulungiswa kweSampuli yeGazi:
Ngaphambi kokwenza i-centrifugation, ukulungiselela ngononophelo isampuli yegazi kuyimfuneko. Kubalulekile ukuqokelela umthamo owaneleyo wegazi kwityhubhu efanelekileyo ye-anticoagulant, njengetyhubhu ye-EDTA, ukuthintela ukujiya kwegazi. Isampuli kufuneka iguqulwe kancinci amaxesha amaninzi ukuqinisekisa ukuxubana okufanelekileyo kwe-anticoagulant negazi. Ukongeza, nawaphi na amahlwili abonakalayo kufuneka asuswe, njengoko anokuthintela inkqubo yokwahlukana kwaye onakalise i-serum efunyenweyo.
Iimeko zokufaka i-Centrifugation:
Ukuze kufezekiswe ukwahlukana okusebenzayo, kufuneka kusetyenziswe iimeko ezifanelekileyo zokucoca i-centrifugation. Isantya kunye nobude be-centrifugation zezona zinto zibalulekileyo ezidlala indima ebalulekileyo ekufumaneni i-serum ecacileyo necocekileyo. Ngokubanzi, isantya se-3,000 revolutions ngomzuzu (rpm) siyacetyiswa xa kuhlulwa i-serum, nangona oku kunokwahluka ngokuxhomekeke kwi-centrifuge ethile kunye nohlobo lwesampuli. Ukongeza, ixesha lokucoca i-centrifugation liphakathi kwemizuzu eli-10 ukuya kweli-15, nto leyo evumela ixesha elaneleyo lokwahlula i-serum ngokupheleleyo.
Ukuqokelela iSerum:
Nje ukuba i-centrifugation igqityiwe, i-serum ehlukanisiweyo ingaqokelelwa ngononophelo. Kubalulekile ukuphatha iityhubhu ngononophelo ukuze ungaphazamisi i-pellet. Usebenzisa i-transfer pipette okanye isixhobo esikhethekileyo sokuqokelela i-serum, khupha i-serum ngobunono ngaphandle kokufunxa nayiphi na i-pellet okanye naziphi na iiseli ezibomvu zegazi ezinokubakho kwindawo yokuhlangana. Khumbula ukuba ungcoliseko lwe-serum ngeeseli ezibomvu zegazi lunokukhokelela kwiziphumo zovavanyo ezingachanekanga kwaye luthintele ukusetyenziswa kwayo ngezantsi.
Izicelo zoKwahlulwa kweSerum:
Ukwahlulwa kwe-serum kwenziwa ngokubanzi ngeenjongo zokuxilonga, uphando, kunye nonyango. Ekuxilongweni kwezonyango, uhlalutyo lwe-serum luvumela ukufunyanwa kweempawu ezithile ezinxulumene nezifo, ukunceda ekuxilongweni ngokuchanekileyo nasekubekweni esweni kwezigulane. Abaphandi basebenzisa ne-serum eyahlukileyo ukufunda iindlela zesifo, ukuvavanya ukusebenza kakuhle konyango, kunye nokuchonga ii-biomarkers ezinokubakho. Ngaphezu koko, ukwahlulwa kwe-serum kubalulekile kwizicelo zonyango, ezinje ngokutofelwa igazi kunye nokuveliswa kweemveliso ezahlukeneyo ezithathwe egazini.
Izinto ekufuneka uziqwalasele ngokhuseleko:
Nangona i-centrifugation iyindlela eqhelekileyo, ineengozi ezithile ekufuneka zijongwane nazo. Ukujikeleza ngesantya esiphezulu kunokubangela ukungalingani kwi-centrifuge, okukhokelela ekungcangcazeleni okuyingozi okanye nasekusileleni kwezixhobo. Ke ngoko, soloko ufaka iityhubhu ezilinganayo kwi-rotor kwaye uhlole rhoqo i-centrifuge ukuba kukho iimpawu zokuguguleka okanye umonakalo. Ukongeza, xa uphatha iisampulu zegazi kwaye usebenza ne-centrifuge, kubalulekile ukulandela imigaqo efanelekileyo yokhuseleko lwelabhoratri ukunciphisa nawuphi na umngcipheko weengozi zebhayoloji.
Isiphelo:
Ukukwazi ukwahlula i-serum egazini kusetyenziswa i-centrifuge kubaluleke kakhulu kwiindawo ezininzi zonyango nezenzululwazi. Le nkqubo ivumela ukwahlulwa kunye nohlalutyo lwezinto ezibalulekileyo ezikhoyo kwi-serum, inika ulwazi olubalulekileyo lokuxilongwa, uphando, kunye nokusetyenziswa konyango. Ngokuqonda imigaqo, ukukhetha i-centrifuge efanelekileyo, ukusebenzisa iimeko ezifanelekileyo zokucoca, kunye nokulandela izikhokelo zokhuseleko, izazinzulu kunye noogqirha banokuhlula i-serum egazini ngempumelelo, bavule ulwazi oluninzi lokuphucula ukhathalelo lwezigulane, baphuhlise unyango olutsha, kwaye bandise ukuqonda kwethu ngezifo ezahlukeneyo.
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