yuav ua li cas cais cov serum ntawm cov ntshav nrog lub centrifuge

Kev Taw Qhia:

Cov tshuab centrifuges ua lub luag haujlwm tseem ceeb hauv ntau yam kev tshawb fawb thiab kev kho mob, uas tso cai rau cov kws tshawb fawb thiab cov kws kho mob cais cov khoom sib xyaw raws li lawv qhov ceev. Ib qho kev siv tseem ceeb ntawm centrifugation yog kev sib cais cov serum ntawm cov ntshav. Cov txheej txheem no muab cov kev nkag siab tseem ceeb rau tus neeg mob txoj kev noj qab haus huv los ntawm kev cais thiab tshuaj xyuas cov protein, enzymes, hormones, thiab lwm yam khoom uas muaj nyob hauv serum. Hauv tsab xov xwm no, peb yuav tshawb nrhiav cov txheej txheem ntawm kev sib cais serum ntawm cov ntshav siv lub centrifuge, qhia txog nws qhov tseem ceeb hauv kev kuaj mob, kev tshawb fawb, thiab kev siv kho mob.

Kev Nkag Siab Txog Centrifugation:

Kev siv lub zog centrifugal los cais cov khoom sib txawv. Los ntawm kev tig cov qauv ntawm qhov ceev ceev, kev siv lub zog centrifugal ua rau cov khoom me me nyob hauv qab, tsim cov pellet, thaum cov khoom sib dua tseem nyob hauv cov supernatant. Yuav kom ua tiav kev sib cais, nws yog ib qho tseem ceeb kom nkag siab txog cov ntsiab cai thiab cov yam ntxwv uas cuam tshuam rau kev siv lub zog centrifugal.

Xaiv Lub Centrifuge Zoo:

Kauj ruam thawj zaug hauv kev cais cov ntshav tawm ntawm cov ntshav yog xaiv lub centrifuge uas tsim nyog. Cov centrifuge sib txawv muaj peev xwm sib txawv, xws li qhov loj me ntawm rotor sib txawv, qhov ceev tshaj plaws, thiab qhov chaw teeb tsa programmable. Rau kev cais cov ntshav, centrifuge nrog lub rotor swing-out feem ntau yog qhov zoo dua, vim nws tso cai rau kev sau cov khoom sib cais yam tsis cuam tshuam cov pellet. Tsis tas li ntawd, xyuam xim rau lub centrifuge lub peev xwm siab tshaj plaws g-force, thiab xyuas kom meej tias nws ua tau raws li qhov yuav tsum tau ua rau kev cais cov ntshav.

Kev Npaj Cov Qauv Ntshav:

Ua ntej centrifugation, yuav tsum npaj cov ntshav kuaj kom zoo. Nws yog ib qho tseem ceeb kom sau cov ntshav kom txaus rau hauv lub raj mis anticoagulant uas tsim nyog, xws li lub raj mis EDTA, kom tiv thaiv kev txhaws. Cov ntshav kuaj yuav tsum tau tig maj mam ob peb zaug kom ntseeg tau tias cov tshuaj anticoagulant sib xyaw nrog cov ntshav. Tsis tas li ntawd, txhua qhov ntshav txhaws uas pom tau yuav tsum tau tshem tawm, vim tias lawv tuaj yeem cuam tshuam cov txheej txheem sib cais thiab ua rau cov serum tau txais puas tsuaj.

Cov xwm txheej ntawm kev centrifugation:

Yuav kom ua tiav kev sib cais zoo, yuav tsum siv cov xwm txheej centrifugation tsim nyog. Qhov ceev thiab lub sijhawm centrifugation yog cov yam tseem ceeb uas ua lub luag haujlwm tseem ceeb hauv kev tau txais cov serum ntshiab thiab huv. Feem ntau, qhov ceev ntawm 3,000 revolutions ib feeb (rpm) yog qhov pom zoo rau kev sib cais serum, txawm hais tias qhov no yuav txawv nyob ntawm lub centrifuge thiab hom qauv. Tsis tas li ntawd, lub sijhawm centrifugation yog ntawm 10 txog 15 feeb, tso cai rau lub sijhawm txaus rau kev sib cais tag nrho ntawm cov serum.

Sau cov tshuaj serum:

Thaum lub centrifugation ua tiav lawm, cov serum uas tau cais tawm tuaj yeem raug sau kom zoo. Nws yog ib qho tseem ceeb uas yuav tsum tuav cov raj nrog kev ceev faj kom tsis txhob cuam tshuam cov pellet. Siv lub pipette hloov pauv lossis lub cuab yeej sau serum tshwj xeeb, maj mam rho tawm cov serum yam tsis tau nqus cov pellet lossis cov qe ntshav liab uas yuav muaj nyob ntawm qhov sib txuas. Nco ntsoov tias kev ua qias tuaj ntawm cov serum nrog cov qe ntshav liab tuaj yeem ua rau cov txiaj ntsig kuaj tsis raug thiab cuam tshuam rau kev siv tom qab.

Cov ntawv thov ntawm kev sib cais ntawm cov ntshav:

Kev sib cais cov ntshav hauv cov ntshav yog siv rau kev kuaj mob, kev tshawb fawb, thiab kev kho mob. Hauv kev kuaj mob hauv tsev kho mob, kev tshuaj xyuas cov ntshav hauv cov ntshav tso cai rau kev nrhiav pom cov cim tshwj xeeb uas cuam tshuam nrog cov kab mob, pab txhawb kev kuaj mob thiab kev saib xyuas cov neeg mob kom raug. Cov kws tshawb fawb kuj siv cov ntshav uas tau sib cais los kawm txog cov txheej txheem ntawm kev mob, ntsuas qhov ua tau zoo ntawm kev kho mob, thiab txheeb xyuas cov cim qhia txog cov kab mob. Ntxiv mus, kev sib cais cov ntshav hauv cov ntshav yog qhov tseem ceeb hauv kev siv kho mob, xws li kev hloov ntshav thiab kev tsim ntau yam khoom los ntawm cov ntshav.

Kev Xav Txog Kev Nyab Xeeb:

Txawm hais tias centrifugation yog ib txoj kev siv ntau, nws muaj qee qhov kev pheej hmoo uas yuav tsum tau daws. Kev tig ceev ceev tuaj yeem ua rau tsis sib npaug hauv lub centrifuge, ua rau muaj kev co lossis txawm tias cov khoom siv tsis ua haujlwm. Yog li ntawd, ib txwm muab cov raj sib npaug hauv lub rotor thiab tshuaj xyuas lub centrifuge tsis tu ncua rau cov cim ntawm kev hnav lossis kev puas tsuaj. Tsis tas li ntawd, thaum tuav cov qauv ntshav thiab ua haujlwm nrog lub centrifuge, nws yog ib qho tseem ceeb kom ua raws li cov txheej txheem kev nyab xeeb hauv chaw kuaj mob kom txo qis txhua qhov kev pheej hmoo ntawm kev phom sij biohazard.

Xaus lus:

Lub peev xwm cais cov serum tawm ntawm cov ntshav siv lub centrifuge yog qhov muaj nqis heev rau ntau qhov chaw kho mob thiab kev tshawb fawb. Cov txheej txheem no ua rau muaj kev cais thiab tshuaj xyuas cov khoom tseem ceeb uas muaj nyob hauv cov serum, muab cov ntaub ntawv tseem ceeb rau kev kuaj mob, kev tshawb fawb, thiab kev siv kho mob. Los ntawm kev nkag siab txog cov ntsiab cai, xaiv lub centrifuge zoo, siv cov xwm txheej centrifugation tsim nyog, thiab ua raws li cov lus qhia kev nyab xeeb, cov kws tshawb fawb thiab cov kws kho mob tuaj yeem cais cov serum tawm ntawm cov ntshav tau zoo, qhib kev paub ntau yam los txhim kho kev saib xyuas tus neeg mob, tsim cov kev kho mob tshiab, thiab ua kom peb nkag siab tob txog ntau yam kab mob.