isetyenziswa njani i-centrifuge ukwahlula igazi
Intshayelelo kwiCentrifugation kunye nezicelo zayo kuLwahlulo lweGazi
I-Centrifugation yindlela esetyenziswa kakhulu kwiindawo ezahlukeneyo zesayensi nezonyango, kwaye enye yeendlela zayo ezibalulekileyo kukwahlulahlula iinxalenye zegazi. Le nkqubo idlala indima ebalulekileyo ekuxilongweni kwezonyango, kuphando, nakwizicelo zonyango. Ngokusebenzisa umgaqo wamandla e-centrifugal, i-centrifuge yahlula igazi ngokufanelekileyo kwiindawo zayo, kuquka iiseli ezibomvu zegazi, iiseli ezimhlophe zegazi, i-plasma, kunye neeplatelets. Kweli nqaku, siza kuhlola indlela i-centrifuge esetyenziswa ngayo ukwahlula igazi kwaye siqonde ukubaluleka kwale nkqubo kwezonyango.
Ukuqonda uMgaqo oSebenzayo weCentrifuge
Ukuze uqonde inkqubo yokwahlulwa kwegazi usebenzisa i-centrifuge, kubalulekile ukuqonda umgaqo osisiseko osebenzayo emva kwalo matshini unamandla. I-centrifuge sisixhobo esijikeleza ngesantya esiphezulu, senza amandla e-centrifugal abangela ukubola kunye nokwahlukana kwezinto ezahlukeneyo ngokusekelwe kubuninzi bazo kunye nobukhulu bazo. Amandla e-centrifugal afunyenwe ngaphakathi kwe-rotor ye-centrifuge atyhala amasuntswana anzima ukuya kwimiphetho engaphandle, ngelixa amasuntswana akhanyayo ehlala ekufutshane nombindi.
Iityhubhu zoKwahlula igazi kunye nendima yazo kwiCentrifugation
Iityhubhu zokwahlukanisa igazi zizikhongozeli ezenzelwe ngokukodwa ezenza kube lula inkqubo yokuxuba igazi. Ezi tyhubhu ziqulathe izinto ezithintela ukujiya kwegazi, ezithintela ukujiya kwegazi ngexesha lokugcina nokwahlukanisa. Kukho iintlobo ezahlukeneyo zeetyhubhu zokwahlula igazi, nganye isebenza ngeenjongo ezithile. Umzekelo, iityhubhu zokwahlula igazi ziqulathe izongezo zokuthintela ukujiya kwegazi kwaye zenze kube lula ukwahlula iplasma kwizinto zeseli. Kwelinye icala, iityhubhu zokwahlula igazi zenzelwe ukukhuthaza ukujiya kwegazi, okuvumela ukwahlukana kwe-serum kwiiseli zegazi. Ukukhethwa kwetyhubhu yokwahlula igazi efanelekileyo kuxhomekeke kwisiphumo esifunekayo senkqubo yokuxuba igazi.
Inkqubo yeCentrifugation yoKwahlulwa kweGazi
Ukuze igazi lahlulwe libe ngamacandelo alo, inyathelo lokuqala kukufumana isampuli yegazi kwisigulana. Emva kokuqokelelwa, isampuli yegazi ixutywa ngobunono ukuqinisekisa ukusasazwa ngokufanelekileyo kwe-anticoagulant okanye i-clot activator ngaphakathi kwityhubhu. Nje ukuba ixutywe, ityhubhu ifakwa ngononophelo kwi-centrifuge, ukuqinisekisa ukuba ilinganiswe ngokufanelekileyo. Iityhubhu ezingalinganiyo zinokukhokelela ekuhlukaneni okungasebenzi kakuhle okanye zide zonakalise i-centrifuge.
Xa i-centrifuge iqala ukujikeleza ngesantya esiphezulu, amandla e-centrifugal asebenza kwisampulu yegazi. Izinto ezisegazini ezikhoyo, ezifana neeseli ezibomvu zegazi, ezixineneyo, zinyanzelwa ukuya ezantsi kwetyhubhu, zenze i-pellet okanye i-sediment. Kwangaxeshanye, izinto ezikhaphukhaphu ezifana ne-plasma ziyanyuka ziye phezulu. Isantya kunye nobude be-centrifugation zizinto ezibalulekileyo ezimisela ukusebenza kakuhle kokwahlukana. Ezi parameters ziyahluka ngokuxhomekeke kuhlobo lwe-centrifuge kunye nesiphumo esifunekayo sokwahlukana.
Ukufunyanwa kweCandelo leGazi kunye nokuLisebenzisa kwiindlela ezahlukeneyo zokusetyenziswa
Nje ukuba inkqubo ye-centrifugation igqityiwe, izinto ezahlulweyo zilungele uhlalutyo olongezelelweyo, uvavanyo, okanye iinkqubo zonyango. Umzekelo, iqhekeza le-plasma elifunyenwe kwinkqubo ye-centrifugation lityebile kwiiproteni, izinto ezibangela ukugabha igazi, kunye nee-antibodies. Lisetyenziswa rhoqo kwiimvavanyo zokuxilonga, ezinje ngokufumanisa amanqanaba e-cholesterol, ukujonga ukusebenza kwesibindi, okanye ukufumanisa izifo ezosulelayo. Iiseli ezibomvu zegazi, kwelinye icala, zinokusetyenziselwa utofelo-gazi okanye kuphando olunxulumene neengxaki zegazi. Iiseli ezimhlophe zegazi kunye neeplatelets ezifunyenwe kwiindlela zokwahlulwa zinezicelo zazo ezizodwa kwiinkqubo zonyango ezikhethekileyo.
Ukuqukumbela, i-centrifugation yindlela ebalulekileyo esetyenziselwa ukwahlula igazi libe ngamacandelo alo, nto leyo evumela ukusetyenziswa okuninzi kwezonyango. Ngokusebenzisa amandla e-centrifugal force, le nkqubo inceda abaphandi, oogqirha, kunye neengcali zokuxilonga ukuba baphande ngezifo, bajonge impilo yezigulane, kwaye baphuhlise unyango olutsha. Ukuqonda ubunzima be-centrifugation kunye nokusetyenziswa kwayo ekuhlukaniseni igazi kunika ulwazi oluxabisekileyo ngendima ebalulekileyo edlalwa yile ndlela kwicandelo lezempilo.
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