i-centrifuge iyahlula njani igazi
Intshayelelo yoKwahlulwa kweGazi kunye neCentrifugation
Umgaqo Osebenzayo WeeCentrifuge Ekwahlulweni Kwegazi
Iintlobo zeeCentrifuges ezisetyenziselwa ukwahlula igazi
Inkqubo Yokwahlula Iinxalenye Zegazi kusetyenziswa iCentrifugation
Izicelo kunye neenzuzo zeCentrifugation kwiSahlulo seGazi
Intshayelelo yoKwahlulwa kweGazi kunye neCentrifugation
Igazi liyinxalenye ebalulekileyo yomzimba womntu, elenza imisebenzi eyahlukeneyo efana nokuthuthwa kweoksijini, ukususwa kwenkunkuma, kunye nokusabela komzimba. Liqulathe izinto ezahlukeneyo zeseli nezingezizo ezeseli, kuquka iiseli zegazi ezibomvu nezimhlophe, iiplatelets, iplasma, iihomoni, kunye nee-enzymes. Ukuze kufundwe ezi zinto ngokwazo ngeenjongo zokuxilonga okanye zophando, kuyimfuneko ukuzihlukanisa. Kulapho ke kusetyenziswa khona i-centrifugation.
Umgaqo Osebenzayo WeeCentrifuge Ekwahlulweni Kwegazi
I-Centrifugation yinkqubo esebenzisa imigaqo yamandla e-centrifugal ukwahlula izinto zomxube ngokusekelwe kubuninzi bazo kunye nobukhulu bazo. I-centrifuge sisixhobo esikhethekileyo esijikeleza ngesantya esiphezulu, senza intsimi ye-centrifugal. Xa igazi liphantsi kwala mandla, izinto ezixineneyo ziyasuka embindini wokujikeleza, ngelixa izinto ezingaxineneyo ziqokelelana kufutshane nombindi. Olu hluko luvumela ukwahlulwa kwezinto ezithile ezinomdla.
Iintlobo zeeCentrifuges ezisetyenziselwa ukwahlula igazi
Kukho iintlobo ngeentlobo zee-centrifuge ezisetyenziselwa ukwahlula igazi, kuquka ii-tabletop centrifuge, ii-floor-model centrifuge, ii-ultracentrifuge, kunye nee-microcentrifuge. Ii-tabletop centrifuge zisetyenziswa kakhulu kwiilebhu zeklinikhi ngenxa yokuguquguquka kwazo kunye nobukhulu obuncinci. Ii-floor-model centrifuge zikhulu kwaye zinamandla ngakumbi, zihlala zisetyenziswa kwiibhanki zegazi okanye kwiindawo zophando. Ii-Ultracentrifuge ziyakwazi ukufikelela kwisantya esiphezulu kakhulu kwaye zinokuhlukanisa amasuntswana ngokuchanekileyo okukhulu. Ii-Microcentrifuge zincinci kwaye zenzelwe ukusetyenziswa okuncinci, zihlala zisetyenziswa kuphando lwemfuza okanye kwiilebhu ze-microbiology.
Inkqubo Yokwahlula Iinxalenye Zegazi kusetyenziswa iCentrifugation
Ukwahlula iinxalenye zegazi usebenzisa i-centrifuge, inyathelo lokuqala kukuqokelela isampuli yegazi kwisikhongozeli esifanelekileyo, njengetyhubhu yokuvavanya okanye ityhubhu ekhethekileyo yokuqokelelwa kwegazi. Le sampuli emva koko ifakwa kwi-centrifuge rotor, elungelelaniswe ukuze kuthintelwe naziphi na iingxaki ngexesha lokusebenza. Emva kokuvala isiciko se-centrifuge, umatshini umiselwa kwisantya nexesha elifunekayo. Uqala ukujikeleza, uvelise amandla e-centrifugal asebenza kwisampuli yegazi.
Ngexesha lokuxuba igazi, izinto ezixineneyo zegazi, ezifana neeseli ezibomvu zegazi, ziyahamba ziye ezantsi kwetyhubhu ngenxa yobuninzi bazo. Eli candelo laziwa ngokuba yi-sediment okanye i-pellet. Ngaphezulu kwe-sediment, umaleko weeseli ezimhlophe zegazi kunye neeplatelets nawo unokwenziwa. Ulwelo oluseleyo, olwaziwa ngokuba yi-plasma, aluxinene kangako kwaye luqokelelana luye phezulu. Ubude be-centrifugation buyahluka ngokuxhomekeke kwinqanaba elifunekayo lokwahlukana.
Xa i-centrifuge iyeka ukusebenza, izinto ezahluliweyo zingaqokelelwa ngononophelo. Umzekelo, i-pipette ingasetyenziselwa ukukhupha i-plasma okanye umaleko ongaphezulu weeseli. Izinto eziqokelelweyo zingasetyenziselwa uvavanyo olongezelelweyo, uhlalutyo, okanye ukugcinwa. Ngokwahlula izinto zegazi kusetyenziswa i-centrifugation, izazinzulu kunye neengcali zempilo zinokufumana ulwazi oluxabisekileyo ngezifo ezahlukeneyo, zijonge impilo yezigulane, kwaye zenze uphando malunga nonyango olutsha.
Izicelo kunye neenzuzo zeCentrifugation kwiSahlulo seGazi
Ukucoca igazi nge-centrifugation kudlala indima ebalulekileyo kwiindlela ezininzi zonyango. Kwiilabhoratri zeklinikhi, isetyenziselwa uvavanyo lwegazi oluqhelekileyo, okuvumela ukulinganiswa kwezinto ezifana ne-hematocrit, emisela ipesenti yeeseli ezibomvu zegazi kwisampulu. Ngaphezu koko, inceda ekuxilongweni nasekujongeni iingxaki ezahlukeneyo zegazi kunye nosulelo. Ukucoca igazi nge-centrifugation kusetyenziswa nakwiibhanki zegazi ukwahlula igazi lilonke libe ngamacandelo alo, njengeeseli ezibomvu zegazi, i-plasma, kunye neeplatelets, okuvumela utofelo-gazi olukhuselekileyo nolusebenzayo.
Ngaphaya kweemeko zonyango, i-centrifugation isetyenziswa kwiilabhoratri zophando ukuphanda iingxaki ezinxulumene negazi kunye nokuphonononga iindlela zonyango ezinokubakho. Ivumela ukwahlulwa kweeseli ezithile zegazi, iququzelela izifundo ezineenkcukacha malunga nolwakhiwo lwazo, umsebenzi, kunye nokusabela kwizikhuthazo. Ukongeza, i-centrifugation isetyenziswa kwishishini lamayeza ukucoca nokwahlula iimveliso ezivela egazini, kubandakanya iiproteni zeplasma kunye nezinto ezibangela ukugabha kwegazi, ezibalulekileyo kuphuhliso lwamayeza kunye nonyango olwahlukeneyo.
Ukuqukumbela, i-centrifugation yindlela enamandla esetyenziselwa ukwahlula iinxalenye zegazi ngokusekelwe kubuninzi bazo kunye nobukhulu bazo. Ngokusebenzisa imigaqo yamandla e-centrifugal, i-centrifuges inokwahlula ngempumelelo iiseli zegazi ezibomvu nezimhlophe, iiplatelets, kunye ne-plasma, nto leyo evumela imizamo ebalulekileyo yokuxilonga kunye nophando. Nokuba luvavanyo lwezonyango oluqhelekileyo, utofelo-gazi, okanye ukuphuhlisa unyango lwexesha elizayo, i-centrifugation iyaqhubeka nokuguqula intsimi yokwahlulwa kwegazi, iqhubela phambili ukuqonda kwethu kwaye iphucula ukhathalelo lwezigulane.
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