i-centrifuge ilahlula njani igazi
I-Centrifuge ilahlula njani igazi?
Intshayelelo:
Ukwahlulwa kwegazi yinkqubo ebalulekileyo kwiinkqubo ezahlukeneyo zonyango nezenzululwazi. Esinye sezixhobo ezisetyenziswa kakhulu kule njongo yi-centrifuge. Kweli nqaku, siza kuhlola indlela i-centrifuge eyahlula ngayo igazi kunye nokubaluleka kwalo kwiindlela ezahlukeneyo ezifana novavanyo lokuxilonga, i-blood banking, kunye nophando lwe-biomedical. Siza kujonga indlela yokwahlula igazi sisebenzisa i-centrifuge kwaye sixoxe ngeentlobo ezahlukeneyo ze-centrifuge ezisetyenziswa kweli candelo. Ngoko ke, makhe sijonge kwihlabathi le-centrifuge kunye nendima yalo ekwahlukaniseni iinxalenye zegazi.
Ukuqonda Izinto Eziyinxalenye Yegazi
Ngaphambi kokuba sixoxe ngendlela ii-centrifuges ezahlula ngayo igazi, kubalulekile ukuba nolwazi olusisiseko ngezinto ezisetyenziswa kwigazi. Igazi liquka ikakhulu iiseli ezibomvu zegazi (ii-erythrocytes), iiseli ezimhlophe zegazi (ii-leukocytes), iiplatelets (ii-thrombocytes), kunye ne-plasma. Icandelo ngalinye lisebenza umsebenzi owahlukileyo emzimbeni. I-plasma ithwala izondlo, iihomoni, imveliso yenkunkuma, kunye nee-antibodies, ngelixa iiseli ezibomvu nezimhlophe zegazi zinegalelo ekuthuthweni kweoksijini kunye neempendulo zomzimba ngokwahlukeneyo.
Indima yeCentrifugation ekwahlulweni kwegazi
I-Centrifugation yindlela esebenzisa amandla e-centrifugal ukwahlula amasuntswana akhoyo kulwelo ngokusekelwe kubuninzi bawo. Kwimeko yokwahlulwa kwegazi, i-centrifuge isetyenziswa ukwahlula izinto ezikhankanyiweyo ngaphambili. Ngokubeka isampuli yegazi kujikelezo olukhawulezayo, i-centrifuge idala amandla e-centrifugal anamandla atyhala amasuntswana axineneyo, njengeeseli ezibomvu zegazi, ukuya ezantsi, zenze i-pellet, ngelixa izinto ezingaxineneyo kangako, njenge-plasma, zihlala ziphezulu.
Iintlobo zeeCentrifuges ezisetyenziswa ekwahlulweni kwegazi
Kukho iintlobo ezahlukeneyo zee-centrifuge ezisetyenziselwa ukwahlula igazi, kuquka ii-benchtop centrifuge kunye nee-centrifuge ezifrijiweyo. Ii-benchtop centrifuge zisetyenziswa kakhulu kwiilebhu zeklinikhi zovavanyo lwegazi oluqhelekileyo, ngelixa ii-centrifuge ezifrijiweyo zisetyenziselwa ukugcina amaqondo obushushu aphantsi ngexesha lokwahlula, ukuqinisekisa uzinzo kwiindawo ezinobushushu. Zombini ezi ntlobo zisebenza ngemigaqo efanayo, zisebenzisa intshukumo yokujikeleza ngesantya esiphezulu ukuvelisa amandla afunekayo e-centrifugal.
Inkqubo yeCentrifugation
Inkqubo yokwahlulwa kwegazi kusetyenziswa i-centrifuge ibandakanya amanyathelo aliqela. Okokuqala, isampuli yegazi iyaqokelelwa ize idluliselwe kwityhubhu efanelekileyo, njengetyhubhu ye-conical centrifuge, enokumelana nesantya esiphezulu sokujikeleza esifunekayo. Kubalulekile ukuphatha isampuli ngononophelo ukuqinisekisa iziphumo ezichanekileyo nezithembekileyo. Nje ukuba isampuli iqiniswe kwityhubhu, ifakwa kwi-centrifuge, kwaye isiciko sivalwe ngokukhuselekileyo.
Kwinyathelo elilandelayo, i-centrifuge icwangciswe ngezicwangciso ezithile ezifana nexesha, isantya, kunye nokukhawulezisa. Ezi zicwangciso zimiselwa ngokusekelwe kwisiphumo esifunekayo kunye nezahlulo. Njengoko i-rotor iqala ukujikeleza, i-centrifuge ivelisa amandla e-centrifugal afunekayo, nto leyo ebangela ukuba izinto ezixineneyo ngaphakathi kwegazi, njengeeseli ezibomvu zegazi, zahlukane nezinto ezingaxineneyo kangako. Emva kwexesha elimiselweyo, i-centrifuge iyema.
Ukuqokelelwa kunye noHlalutyo lweeCandelo ezahlulweyo
Emva kokuba i-centrifuge igqibile ukujikeleza kwayo, izinto ezahlulweyo zinokuqokelelwa kwaye zihlalutywe ngakumbi. Ngokuxhomekeke kwisicelo esifunekayo, izinto ezahlulweyo zinokudluliselwa kwiityhubhu ezahlukeneyo okanye kwiikhonteyina ukuze ziqhubeke nokucutshungulwa. Umzekelo, ukuba injongo ephambili kukufumana i-plasma, inokwahlulwa ngononophelo kwezinye izinto kusetyenziswa i-pipette okanye esinye isixhobo esifanelekileyo. Ngokufanayo, ukuba izinto ezithile ezifana ne-buffy coat (ezenziwe ngeeseli ezimhlophe zegazi kunye neeplatelets) ziyafuneka, zinokukhutshwa kwaye zicutshungulwe ngokufanelekileyo.
Isiphelo:
Ukucoca igazi ngendlela ye-centrifugation yindlela ebalulekileyo yokwahlula igazi, okuvumela izazinzulu kunye neengcali zonyango ukuba zifumane izinto zegazi ezicociweyo kwiindlela ezahlukeneyo zokusetyenziswa. Ngokusebenzisa imigaqo yamandla e-centrifugal, le nkqubo ivumela ukwahlulwa nokuqokelelwa kwezinto ezahlukeneyo, ezifana ne-plasma, iiseli zegazi ezibomvu, kunye neeseli zegazi ezimhlophe. Ukukwazi ukwahlula igazi ngempumelelo nge-centrifuge kuye kwaphucula kakhulu ukuxilongwa, uphando, kunye neenkqubo zonyango, kuphucula ukhathalelo lwezigulane kwaye negalelo kwinkqubela phambili yesayensi kwicandelo lezonyango.
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