indlela esebenza ngayo i-centrifuge xa kuhlukaniswa
Indlela iCentrifuge esebenza ngayo xa kwahlukana
Intshayelelo kwiCentrifugation kunye nokubaluleka kwayo kwiinkqubo zokwahlukana
I-Centrifugation yindlela ebalulekileyo esetyenziswa kwiinkalo ezahlukeneyo zesayensi, kuquka ikhemistri, ibhayoloji, kunye neyeza, ukwahlula amasuntswana aqinileyo okanye imixube yolwelo nolwelo. Le ndlela inamandla nesebenzayo isebenzisa imigaqo yamandla e-centrifugal ukufezekisa ukwahlukana. Kweli nqaku, siza kujonga nzulu kwindlela yokusebenza emva kwe-centrifuge kwaye sihlolisise ukubaluleka kwayo kwiinkqubo zokwahlukana.
Ukuqonda uyilo kunye neenxalenye zeCentrifuge
I-centrifuge idla ngokuba ne-rotor eqhutywa yi-motor, i-sample holder, kunye nesiciko okanye isiciko ukuqinisekisa ukhuseleko lomqhubi. I-rotor yinxalenye ebalulekileyo enoxanduva lokuvelisa amandla e-centrifugal. Ijikeleza ngesantya esiphezulu, ngokuqhelekileyo amawaka ee-revolutions ngomzuzu (RPM), inika amandla afunekayo okusasaza okanye ukwahlulahlula.
Indima yamandla eCentrifugal ekwahlukaneni
Amandla e-Centrifugal ngamandla abonakalayo asebenza kwizinto ezihamba ngendlela ejikelezayo. Xa umxube ufakwa ngaphakathi kwe-rotor ejikelezayo, eyona nxalenye ixineneyo kumxube iya ngasemdeni ongaphandle ngenxa yokutyhala kwangaphandle okubangelwa ngamandla e-centrifugal. Okwangoku, izinto ezikhaphukhaphu ziya ngasembindini, zenze iileya ezahlukeneyo.
Iintlobo zeeCentrifuges kunye nezicelo zazo
Kukho iintlobo ezahlukeneyo zee-centrifuge ezikhoyo, nganye ifanelekile kwiinkqubo ezahlukeneyo zokwahlula kunye neentlobo zeesampuli. Ezinye iintlobo eziqhelekileyo ziquka ii-differential centrifuge, ii-preparative centrifuge, ii-ultracentrifuge, kunye nee-analytical ultracentrifuge. Ii-differential centrifuge zisetyenziswa kakhulu kwiilabhoratri zonyango zokufaka i-sedimentation kwiisampuli zegazi, ngelixa ii-preparative centrifuge zisetyenziselwa ukwahlula izinto ezithile ngobuninzi obukhulu.
Kwelinye icala, ii-Ultracentrifuges zinokufikelela kwisantya esiphezulu kakhulu kwaye zisetyenziswa kwiindawo zophando ezifana ne-biochemistry kunye ne-molecular biology. Ezi matshini zinamandla kakhulu zinokulinganisa umahluko omncinci kwiisisindo zeemolekyuli kwaye zincede abaphandi bafunde ii-macromolecules ngokuchanekileyo okuphezulu. Ii-analytical ultracentrifuges zixhotyiswe ngeenkqubo ze-optical ezivumela uhlalutyo lwexesha langempela lwamazinga okutsalwa kwetyuwa, ziququzelela uphononongo oluneenkcukacha lwentsebenziswano yee-macromolecule kunye notshintsho lwesakhiwo.
Ukuhamba komsebenzi kunye nokuphucula ukusebenza kakuhle
Ukuze kwenziwe ukwahlula kusetyenziswa i-centrifuge, isampuli kuqala yongezwa kwiityhubhu okanye kwiikhonteyina ezifanelekileyo ezenzelwe ukungena ngaphakathi kwe-rotor. Emva kokubopha isiciko, i-centrifuge imiselwa kwisantya kunye nobude obufunekayo. Njengoko i-rotor ijikeleza, amasuntswana anzima okanye izinto ezikumxube ziqala ukuhlala ezantsi, zenze i-pellet. Kwangaxeshanye, izinto ezikhaphukhaphu zenza iileya ngaphezulu kwe-pellet ngokusekelwe kubuninzi bazo.
Ukuphuculwa kokusebenza kakuhle kuye kwasetyenziswa kwii-centrifuges zanamhlanje ukuphucula iziphumo zokwahlulahlula. Oku kuquka uyilo lwe-rotor oluqinileyo nolukhuselekileyo, iinkqubo zolawulo eziphambili zesantya esichanekileyo kunye nexesha, kunye nokukwazi ukwenza iinkqubo ngokuzenzekelayo ukuze ziphumeze kakhulu, ukunciphisa iimpazamo zomntu kunye nokwandisa ukusebenza kakuhle.
Ukusetyenziswa kweCentrifugation kwiinkalo ezahlukeneyo zesayensi
I-Centrifugation isetyenziswa kwiinkalo ezahlukeneyo zesayensi. Kuphando lwezonyango, i-centrifugation isetyenziselwa ukwahlula i-DNA, i-RNA, kunye neeproteni kwiisampuli. Ivumela ukwahlulahlula izinto ezahlukeneyo zeseli, ezifana ne-mitochondria okanye i-nuclei. Kwishishini lezemithi, i-centrifugation idlala indima ebalulekileyo ekukhutshweni nasekucocweni kweekhompawundi ezahlukeneyo ngexesha lophuhliso lwamayeza.
Kwisayensi yokusingqongileyo, i-centrifugation inceda ekukhupheni nasekuhlalutyeni ungcoliseko oluvela kwiisampuli zamanzi nomhlaba. Ikwayindlela ebalulekileyo ekuveliseni i-biofuels, apho inceda ukwahlula ioyile okanye i-ethanol kwi-biomass. Ukongeza, i-centrifugation isetyenziswa kumashishini okutya neziselo ukwahlula izinto eziqinileyo kwizinto ezimanzi okanye ukwahlula ubuninzi bolwelo ngeenjongo zokulawula umgangatho.
Ukuqukumbela, i-centrifugation yindlela enamandla yokwahlula esetyenziswa kakhulu kwiinkalo zesayensi ezifuna ukwahlulwahlulwa kunye nokucocwa kwemixube. Ngeentlobo ezahlukeneyo ze-centrifuges ezisetyenziswayo namhlanje kunye nokuqhubela phambili okuqhubekayo kubuchwepheshe, ukusebenza kakuhle kunye nokuchaneka kokwahlulwa okufezekisiweyo kuyaqhubeka nokuhambela phambili, okuvumela impumelelo kuphando kunye nokuvelisa izinto ezintsha kumashishini amaninzi.
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